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Home > Effect of Recombinant Bovine Somatotropin Rbst and Oxytocin on Health Indicators, Reproductive Performance and Milk Composition of Nili Ravi Buffaloes Bubalis Bubalis During Spring and Summer Seasons

Effect of Recombinant Bovine Somatotropin Rbst and Oxytocin on Health Indicators, Reproductive Performance and Milk Composition of Nili Ravi Buffaloes Bubalis Bubalis During Spring and Summer Seasons

Thesis Info

Access Option

External Link

Author

Shahid, Rehmat Ullah

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Theriogenology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9902/1/Rehmat_Ullah_Shahid_Theriogenology_UAF_2016.docx

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725991990

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The present study was designed to determine the effects of recombinant bovine somatotropin (rbST) and oxytocin, alone or in combination, on health biomarkers, reproductive performance and milk composition in lactating Nili Ravi buffaloes. A total of 250 buffaloes were randomly divided into three main groups.In group A, buffaloes were divided into three equal subgroups on the basis of their body condition scores (BCS), A1 (Low BCS), A2 (Medium BCS) and A3 (High BCS). In group B, buffaloes pertaining to milk production potential were divided into subgroups B1 (1-2.9 liters/milking), B2 (3-5.9 liters/milking) and B3 (6-12 liters/milking). In group C, buffaloes subjected to different treatments were divided into subgroups, C1 (control), C2 (oxytocin), C3 (rbST) and C4 (both rbST and oxytocin). The animals in all the subgroups received rbST (Boostin -250) at the dose rate of 35 mg on alternate days by s/c route in combination with oxytocin at the dose rate of 1 ml (10 I.U) I/m before each milking except in subgroup C1 (control), which was given no treatment, subgroup C2 which was given only oxytocin and subgroup C3 was given only rbST. Treatments were started on day 70 postpartum and continued till the end of lactation. Milk and blood samples were collected from animals of each subgroup during spring and summer seasons of study period (March 2013 to July 2014). The samples were stored at -20ᵒC till further processing. The results indicated that body condition score (BCS) of buffaloes treated with rbST and oxytocin significantly affected health biomarkers and milk composition. Serum AST activity and TOS were higher in rbST+ oxytocin treated buffaloes with high BCS, while TAC was higher in low BCS animals, compared to other groups. Milk fat contents were higher in high BCS buffaloes, while milk protein and lactose were higher in medium BCS animals. Higher frequency of brisket swelling and pregnancy rates were recorded in buffaloes with high BCS during spring and summer, respectively. In buffaloes treated with rbST+ oxytocin, milk production status had no effect on health biomarkers, reproductive performance and milk fat and protein contents. However, milk lactose and SNF contents were higher in buffaloes of high production group. Serum activities of liver enzymes and TOS were higher during summer, while TAS was high during spring. Similarly, milk protein and lactose contents were higher during spring than summer. Treatment of buffaloes with oxytocin decreased activities of ALT, AST, TOS and milk yield, while TAC was increased compared to controls. Buffaloes given rbST showed lower values of ALT, TOS and higher TAC than control group. Animals treated with rbST+ oxytocin had higher AST, TOS and lower ALT and TAC compared to controls. Milk fat, protein and lactose contents decreased due to rbST, oxytocin or rbST+ oxytocin treatment. Milk yield was increased after rbST treatment and decreased with oxytocin during spring. However, during summer, milk yield was increased after rbST+ oxytocin treatment, but decreased following treatment with either hormone. In conclusion, milk production was increased with recombinant bovine somatotropin and decreased with oxytocin during spring. Recombinant bovine somatotropin and oxytocin treatments showed adverse effects on general health of Nili-Ravi buffaloes.
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شانِ نورِ صاحبِؐ لولاک یوں سمجھی گئی


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خِلقتِ کُن کی بِنا اِس نُور پر رکھی گئی

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ہر کسی پر پیار آیا ؛ دل سے ہر تلخی گئی

بُت پرستی پر لگائی ضرب حق کچھ اس طرح
پھر نہ آئی لوٹ کر ؛ ایسی مٹی ، ایسی گئی

آپؐ کے در پر سخاوت کو عُروج اِتنا ملا
مانگ لی خیرات جس نے بھی اُسے ملتی گئی

خلوتِ غم میں جسے غمخوار کی یاد آ گئی
آپؐ کی موجودگی میں اُس کی تنہائی گئی

مسجدِ اقصیٰ میں عرفاںؔ! ہر نبیؑ کی ذاتِ پاک
تاجدارِ انبیاؐ کی اِقتدا کرتی گئی

اسلامی قیادت کے بنیادی اصول: سیرت نبویﷺ کی روشنی میں

In Islamic perspective leadership is a great feature and most important quality for achievement of national goals. Therefore, all kinds of national and organizational success are based up on quality of good leadership.  Every group, society, organization, state and even the world at large need good leadership who can lead their supporters and followers and organizations towards  successful physical and spiritual growth and development . It is also a fact that leadership is God gifted thing but we can enhance ability for eldership.  Leadership should not be confused with the role of only those who make headlines but in essence almost everyone have sometimes somewhere, somehow played a leading role. It is also a historical fact that the Holy Prophet Muhammad ﷺ was granted all kind of leadership and He established some important principles of Islamic leadership which are necessary to follow them for successful leadership. These principles are foundation of great achievements which are led by the Holy Prophet ﷺ. It is also observed that the Islamic Leadership principles practiced by the Holy Prophet Muhammad ﷺ and subsequently by his Caliphs and pious followers which if practiced will provide success principles equally useful both for Muslims and for non-Muslims minorities both in an Islamic state and society.  In this paper the efforts are made to elaborate the guiding principles of Islamic leadership in the light of the teachings of Prophet Muhammad ﷺ.

Development of Correlation Between Rock Classification System and Modulus of Deformation

Rock Classification methods are important for the evaluation of different rock parameters to be adopted for Civil Engineering works. The classification of rock mass also helps to optimise detailed investigation requirements of a large area. During preliminary design stage of a project, the classification of rock mass in accordance with one or more systems can be used to establish engineering characteristics of the rock mass. This also helps in estimating the strength and deformability of rock mass. A number of correlations have been developed by various researchers to correlate the rock mass rating values derived from different systems. Usually, rock mass classification data are not always available in a format that can immediately be applied to a specific engineering problem. Therefore, correlations may prove very useful to quickly derive different design parameters. Furthermore, the availability of the correlations between classification systems facilitate quick means of verifying resultant rock mass rating values, without re-calculation of the values. In this research, four main and well known rock mass classification systems i.e. Rock Mass Rating (RMR), Tunnel Quality Index (Q System), Rock Structure Rating (RSR) and Geological Strength Index (GSI) have been applied to the data obtained from Diamer Basha Dam and Kohala Hydropower Project sites and the rocks have been categorized according to the numerical values. New correlations among these classification systems have been developed which can be used for the rocks of northern area of Pakistan. Generally for a large civil engineering projects; i.e. a tunnel or a dam, modulus of deformation is required at many locations to understand the behaviour of the rock. However, sometimes it is not possible to perform several in-situ tests due to time and funds constraints. Hence it is essential to establish some relationship between rock mass classifications and modulus of deformation. Another purpose of such studies is to authenticate the existing correlations being used worldwide. Due to the abovementioned constraints, it may be uneconomical to conduct tests in all critical areas of a single project, especially for a large project having highly random rock characteristics. In such kind of situations, a few large-scale in-situ tests are conducted and correlations are made between the modulus of deformation values obtained from these tests and different classification systems. These kinds of correlations can be used for extrapolating the modulus of deformation which may be a representative of a rock mass condition for other areas of the project. However the selection of locations of the tests should be done very carefully. Empirical correlations between rock mass classification systems and deformation modulus are useful if a range of in-situ modulus values is desired to be established. Also the estimated values can be provided for the design. The correlations also indirectly shape the bases to identify the weak areas in the foundation rock that may affect the structural behaviour. In this research, data obtained from Plate Load tests and Flat Jack tests performed at Diamer Basha Dam and Kohala Hydropower Project have been analyzed to develop the correlations of modulus of deformation with four rock mass classification systems i.e. RMR, Q System, RSR and GSI. The Plate Load tests performed at Basha were on large size plate and deep deformation measurements were made with borehole extensometer installed underneath the plate. Based on the rock mass classifications in the four systems, the rock existing at Basha dam site mainly comprises Fair to Good quality igneous rock while at Kohala site it is classified as Poor to Fair quality of sedimentary rock units. The correlations developed among various rock mass classification systems have good regression coefficients ranging from 0.835 to 0.901 indicating good correlations. During the research the correlations have been developed between deformation modulus and four (4) rock mass classification systems. Two different sites of different quality of rocks have yielded different range of moduli. The correlations developed during present study have been compared with existing correlations and it has been found that generally these correlations are in good comparison with the other correlations. The research will benefit in the design of future hydropower projects of Pakistan in the region, as the developed correlations may be used to estimate the modulus of deformation at early design stages.