Pengaruh Cita Rasa dan Nama Merek terhadap Keputusan Pembelian Produk Rotte Bakery pada Masyarakat Kecamatan Tuah Madani Kota Pekanbaru

Penelitian ini bertujuan  untuk  mengetahui pengaruh cita rasa dan nama merek terhadap keputusan pembelian Rotte Bakery. Penelitian ini dilakukan di Kecamatan Tuah Madani Kota Pekanbaru pada bulan Februari sampai dengan Juni 2022. Sampel dalam penelitian ini adalah masyarakat yang melakukan pembelian Rotte Bakery yang berjumlah 100 orang responden, teknik penentuan sampel menggunakan Accidental Sampling. Penelitian ini menggunakan metode kuantitatif dengan analisis data menggunakan regresi linier berganda. Hasil penelitian menunjukkan bahwa variabel cita rasa dan nama merek memiliki pengaruh yang signifikan terhadap variabel keputusan pembelian produk Rotte Bakery. Secara simultan variabel cita rasa dan merek memiliki pengaruh yang signifikan terhadap variabel keputusan pembelian produk Rotte Bakery pada Mayarakat Kecamatan Tuah Madani Kota Pekanbaru. Sementara nilai R Square sebesar 0,628 menjelaskan cita rasa dan nama merek memiliki pengaruh yang signifikan terhadap variabel keputusan pembelian produk Rotte Bakery pada Mayarakat Kecamatan Tuah Madani sebesar 62,8% sementara 37,2% sisanya dipengaruhi oleh variabel lain yang tidak diteliti dalam penelitian ini

Molecular Characterization of Blb Resistant Rice Germplasm

Bacterial Leaf Blight (BLB) of rice is one of the major bottlenecks that impede rice production across the globe. Development of resistant rice varieties and pathogen starvation, by blocking its nutrient source, are the promising strategies to effectively combat with this disease. In one of the current studies, BLB resistant Super Basmati introgressed lines (SBILs), were both genotypically and phenotypically characterized. Genotypic characterization indicated that, in terms of different gene combinations, Xa4 and Xa21 were found in 7 SBILs, xa5 and Xa21 in 5 SBILs and all three resistance genes were introgressed in 2 SBILs only. Recovery of recurrent parent genome, by 119 polymorphic SSR markers, ranged from 90.04% to 99.7 % while it ranged from 64.76 % to 94.83 % when analyzed using 1725 polymorphic SNP markers. Pathotyping results revealed that three Xoo strains i.e. PXO99, PXO341, PXO349, and PXO349 were the most virulent against all 25 SBILs. Both genotypic and phenotypic evaluation have shown that majority of SBILs have recovered three Basmati grain quality traits i.e. aroma, grain shape and amylose in them. In susceptible rice host, Xoo transcription activator like effector (TALe) binds with effector binding element (EBE) in the promoter of OsSWEET genes. Any alteration in the target promoter sequence greatly reduces binding affinity of TALe. Therefore, in one of the present studies, 3K SNP seek database was mined, in silico, to identify rice lines that contain SNP/InDels at EBE in the promoter of OsSWEET13 and OsSWEET14. We identified two aus rice lines (Ejali and Khama1183) with 2 base pairs deletions and a single nucleotide substitution at EBE in the promoter of OsSWEET13 and OsSWEET14 respectively. Detected natural variations in Ejali and Khama1183 prevented the induction of both OsSWEET13 and OsSWEET14. An RNA sequencing based study was also conducted to unveil the alternate nutrient sources, primary/secondary metabolic pathways targeted by highly adaptable, mutated Xoo strain in the presence a major resistance gene. We identified a total of 301 differentially expressed genes (DEGs) in IRBB4 infected with WTPXO61 and ΔPXO61. In depth analysis of identified DEGs, using MapMan, revealed that genes specifically associated with the synthesis of Trehalose were strongly induced at early and late stages of infection. Hence, to cope up with the ever evolving pathogen, we need to further diversify and combine different resistance strategies in susceptible rice germplasm to gain broad scale, region specific, durable resistance against BLB.