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Home > Efficacy of Mycorrhizal Fungi and Neemex Against Meloidogyne Incognita Kofoid and White Chitwood on Eggplant in Relation to Nutrients Uptake

Efficacy of Mycorrhizal Fungi and Neemex Against Meloidogyne Incognita Kofoid and White Chitwood on Eggplant in Relation to Nutrients Uptake

Thesis Info

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Author

Rehman Khan, Atta Ur

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2015

Thesis Completion Status

Completed

Subject

Botany

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/7743/1/Atta_ur_Rehman_Khan_Pathology_Plant_UAF_2015.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726035224

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Currently chemical nematicides are the major means to manage plant parasitic nematodes but these are costly and injurious to human health and environment. Research was carried out on an important plant parasitic nematode (Meloidogyne incognita) and its management through the integration of mycorrhizal fungi (MF) and neemex during 2011 to 2013 in green house and field conditions. Various experiments for effect of different dose levels of mycorrhizal fungi (100 g, 150 g and 200 g), neemex (5 g, 10 g and 15 g) and their combination on M. incognita infectivity parameters (Number of galls, egg masses, females, J2/root system and J2/100 cm3 soil), plant growth (root weight, root length, shoot weight and shoot length), nutrients (Nitrogen, phosphorus and potassium) uptake were conducted. Effect of mycorrhizal fungi and neemex on invasion and development by M. incognita was evaluated under green house conditions. Protective and curative effect of mycorrhizal fungi and neemex alone and in combination was tested against M. incognita, plant growth and nutrients uptake by eggplant. Systemic and local effect of mycorrhizal fungi and neemex was evaluated by using split root technique under green house. Management of M. incognita by mycorrhizal fungi and neemex was also evaluated in micro plot under field conditions. Number of galls, egg masses, females, J2/root system and J2/100 cm3 soil were recorded the maximum in untreated control when performed in green house as compared to other treatments. While plant growth parameters like root weight was higher in control but less in other treatments. Root length, shoot length and shoot weight was higher in treatments of all experiments as compared to control. Major nutrients (NPK) uptake was the maximum at higher dose of MF as compared to un-inoculated healthy plant and inoculated with neemex. Invasion and development by M. incognita was observed lower in mycorrhizal and neemex treated plant roots while maximum nematode developmental stages were observed in control. Mycorrhizal fungi and neemex either local or systemic successfully reduced the infection of M. incognita. Nematode infectivity parameters were reduced; plant growth and nutrient contents were enhanced by the mycorrhizal fungi and neemex either alone or in combination under field conditions and can be used successfully for the biocontrol of Meloidogyne incognita.
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پہچان

 

رات پہلے بھییہاں آتی رہی

چیختی چنگھاڑتی تاریکیاں

بیڑیوں کا شور، کوڑوں کی صدا

شب کا اندھا حکم، سہمی سی ہوا

رات کو پہچان لیتے تھے سبھی

 

رات پھر آئی ہے

لیکن روشنی کے بھیس میں

دن کے پردے میں اندھیرے فیصلے

خامشی میں چھپ کے آئے

ہونکتے، پھنکارتے

ظلمتوں کے ضابطے

 

پھر بھی دنیا جانتی ہے رات کو

خلق اب پہچانتی ہے رات کو

Progressive and Retrogressive Phenomena of Economic Activity in Africa

This study examines the concept of globalisation in its characteristics ramifications. The research used empiricism or an empirical method of data collection and analysis. The research used feasibility and affordability in gathering of data for the work using empirical approach in which statistics and figures from credible sources are relied on. The data obtained were discussed using empirical methodological analysis where some themes are identified and discussed from the topic of the study using scientific tools such as tables, charts, bars and figures where necessary. The research discovered that globalisation is not a good phenomenon in African continent owing to slow economic growth, trade disequilibrium and parallel development comparatively because of disadvantages in terms of export, volume of trade exchange and low level of technological development. The research recommends that there is an urgent need for the African countries to restrategise and reshape their economic and political interest at the global level through the creation of a competitive means of relevance at the global centre-stage of world economy and politics.

Postharvest Fruit Softening and Quality Management of Mango Cv. Samar Bahisht Chaunsa

Mango is one of the highly perishable fruit that ripe quickly after harvest. Rapid fruit softening, reduced shelf and storage life limits its distribution to distant markets. The aims of current study were to a) optimize storage temperature in relation to fruit softening; b) physiological management of postharvest fruit softening and c) quality of mango cv. Samar Bahisht Chaunsa with exogenous application of various anti-ripening agents. In various independent experiments, postharvest applications of putrescine (PUT; 0.0, 0.1, 1.0, 1.5 or 2.0 mM), salicylic acid (SA; 0.0, 1.0, 2.0, 3.0 or 4.0 mM), oxalic acid (OA; 0.0, 1.0, 3.0 or 2.0 mM) or calcium chloride (CaCl2; 0.0, 1.0, 3.0 or 5.0%) were applied on mango after harvest to assess their impact on delayed fruit ripening, storage life extension, fruit quality, activities of fruit softening and antioxidative enzymes in the pulp tissues throughout the ripening (7-9 days) and cold storage (28 days) periods. In first experiment, during ripening at ambient conditions (25 ± 1°C; 60-65% RH), a gradual rise was noticed in fruit biochemical attributes such as SSC, SSA:TA ratio, total sugars, reducing and non-reducing sugars; however, ascorbic acid contents and TA showed a declining trend. Furthermore, a progressive increase was observed in the activities of fruit softening enzymes including exo- PG, endo-PG and EGase; whilst, PE activity decreased throughout the ripening period. The activities of CAT and POX enzymes along with total antioxidants were noticed at their peaks two days prior to full fruit ripening. In second experiment, among different cold storage temperatures, 12°C was found to be optimum to maintain best fruit quality characters. The activities of fruit softening enzymes were significantly suppressed at 10°C, as compared to 12°C and 14°C. Total phenolic and antioxidant contents and the activities of CAT, POX and SOD enzymes were reported higher at 14°C than 10°C and 12°C during cold storage. In third experiment, exogenous application of PUT (2 mM) treatments inhibited the ethylene production, respiration rate and maintained higher fruit firmness during ripening as well as cold storage. Fruit treated with 2 mM PUT exhibited a significant reduction in the activities of fruit softening enzymes i.e. exo-PG, endo-PG and EGase; however, maintained higher PE activity in pulp tissues during ripening and cold storage period. Total phenolic and antioxidants contents were significantly higher in 2 mM PUT-treated fruit during the entire ripening and cold storage period than control. The activities of antioxidative enzymes (CAT, POX and SOD) were significantly higher in 2 mM PUT-treated fruit during ripening and cold Abstract xxv storage periods. The SSC contents and SSC:TA ratio were lower in PUT-treated fruit; while, TA and ascorbic acid contents showed a reverse trend. In fourth experiment, the higher concentration of SA (4 mM) inhibited the ethylene production, respiration rate and suppressed the activities of fruit softening enzymes (exo-, endo-PG) with higher activities of PE and antioxidative (SOD, POX and CAT) enzymes, TA, ascorbic acid, total phenolic and antioxidant contents during ripening and cold storage period. During fifth experiment, exogenous application of OA (5 mM) reduced the ethylene production, respiration rate and delayed softening of mango fruit. The reduction and delay in fruit softening during ripening and cold storage was associated with higher PE activity or suppressed activity of exo-PG enzyme in pulp tissues of 5 mM OA-treated fruit. The OA-treated fruit (5 mM) showed increased activities of CAT, POX and SOD enzymes with higher levels of total phenolic and antioxidant contents during ripening and cold storage. The OA-treated fruit exhibited lower SSC contents and SSC:TA ratio; while, TA and ascorbic acid contents were higher throughout the ripening as well as cold storage periods. In the sixth experiment, delay in ripening of ‘Samar Bahisht Chaunsa’ mango fruit was associated with CaCl2 treatment mainly due to inhibition of respiration rate and ethylene production. The exo-PG, endo-PG, PE and EGase activities were significantly suppressed in 5% CaCl2-treated fruit, as compared to control throughout the ripening and cold storage periods. The 5% CaCl2 application showed a reduction in oxidative injury caused by reactive oxygen species through increased activities of antioxidative enzymes i.e. CAT, POX and SOD. During the seventh confirmatory trail, postharvest application of various anti-ripening compounds (2 mM PUT, 4 mM SA, 5 mM OA and 5% CaCl2) delayed the ethylene production and maintained an acceptable fruit quality during the course of study. The activities of fruit softening enzymes such as exo-PG, endo-PG, PE and EGase were significantly suppressed by the application of different anti-ripening chemicals throughout the trials. The use of various chemicals demonstrated elevated activities of antioxidative enzymes (CAT, POX and SOD) than control. In nutshell, the exogenous treatment of PUT (2 mM), SA (4 mM), OA (5 mM) or CaCl2 (5%) may extends the postharvest shelf and storage life of ‘Samar Bahisht Chaunsa’ mango fruit. Moreover, fruit treated with 2 mM PUT and 5% CaCl2 suppressed the activities of fruit softening enzymes; while, 2 mM OA and 4 mM SA exhibited higher activities of antioxidative enzymes with better quality of mango fruit.