Zinc (Zn) deficiency is a universal nutrient constraint of agricultural crops produced on alkaline calcareous soils. Crop species and varieties differ genetically in response to Zn applied to root medium. A series of solution and soil culture experiments were conducted to assess the differential Zn utilization efficiency of wheat genotypes having varying genetic attributes. In the first study, twelve wheat genotypes were grown in solution culture at adequate and deficient Zn levels. The plants were harvested twice after transplanting and Zn efficiency traits, such as biomass accumulation, Zn uptake and Zn utilization efficiency were identified. Based on the results, thus generated, the wheat genotypes Sehar-06 and Vatan were categorized as Zn efficient and inefficient respectively and selected for next studies. In second study, the wheat genotypes were grown in plastic pots containing a Zn deficient soil. Plants were harvested at maturity. The harvested plants were analyzed for uptake and distribution of Zn in wheat straw and grain. Similar response of wheat genotypes to Zn stress was observed in soil culture. Sehar-06 was selected as efficient and Vatan was selected and inefficient genotype. In the third study selected wheat genotypes were grown in solution culture to study Zn uptake, transport, and utilization efficiency. For this, plants were harvested twice and shoot samples were separated in older and younger leaves to estimate Zn translocations within plants. Wheat genotype Sehar-06 efficiently translocated Zn from roots and older leaves to younger leaves when subjected to Zn deficiency stress. The forth study involved growing of these genotypes in solution culture with adequate and deficient Zn levels for 40 days to measure root exudates released in solution. Efficient wheat genotype Sehar-06 released significantly higher amount of maleic acid under Zn deficient conditions but no trend was observed in release of fumaric acid. It is hoped that these findings will set forth useful information to categorize the wheat genotypes under study into efficient and inefficient Zn utilizers. This in turn will be helpful for researchers to plan their breeding experiments and to set genotype specific recommendations for Zn deficient soils.