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Engineering of Microbial Fungal Glucoamylases by Chemical Modification Techniques for Industrial Application

Thesis Info

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Author

Riaz, Muhammad

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2010

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1302

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726057156

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Glucoamylase (exo-1,4-α-D-glucan glucanohydrolase: EC 3.2.1.3) yields ß-D- glucose from the non-reducing chain ends of a wide range of polysaccharide substrates and play a tremendous role in the improvement of starch based food products. Non-traditional use of glucoamylases in detergent industry has a great scope of expansion the world over in the coming days. Industrial employment of glucoamylase demands its hardiness to harsh operational environment. Thermo-stable enzymes can withstand extreme environmental conditions and can resist against detergents, organic solvents, temperature & pH. The operational stability of enzymes is of paramount importance for any bioprocess, which can be improved through various protein engineering techniques. With the development of novel procedures that exploit selective and efficient protein chemistry, chemical modification, either alone or combination with other mutagenesis techniques, could make a significant contribution to the development of enzymes that cope with the industrial demands. Various fungal strains (A. niger, A. oryzae, A. tamarii, A. fumigatus, A. flavus and Humicola sp) were grown in submerged cultivation at different temperatures, pH and substrate concentrations in order to optimize GA production. A. niger and Humicola sp were selected for their higher GA production ability as compared to other fungi. The apparent molecular mass of Humicola sp and A. niger GAs determined through SDS-PAGE were 72.8 and 93 kDa. A. niger GA was proved to be much better as compared to Humicola sp GA on the basis of, wider pH range, higher optimum temperature, more turn over (kcat) and higher specificity constant. It also showed 10 fold higher resistance against thermal unfolding and an activation trend against α- chymotrypsin A. niger was decided to be improved through γ-rays treatment with the main objective to enhance the production, stability and function of the enzyme. The 1 kGy γ-rays exposed mutant (M-7) of A. niger was selected on the basis of deoxy-D- glucose resistance. The mutant M-7 was about two fold efficient for extracellular GA production in submerged cultivation on Vogel’s medium containing 4% (w/v) wheat bran, pH 5.0 at 30 oC. The mutant GA had same molecular mass on SDS-PAGE, however on MALDI-TOF a difference of about 814 Da was observed. The mutant GA proved to
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ہجر فراق سوہنے یار دے وچ: ۵

سی حرفی ۔۵
(رسال پور۱۹۹۵)
الف
الٰہی میل ماہی نوں رو رو جندڑی ہاری میں
خاک گھتی سر جامے پھاڑے، پھر دی نت آواری میں
میت نہ ملیا وچ اڈیکاں، رہندی کرماں ماری میں
آکھ حنیف نہ ملیا ماہی، روواں کر کر زاری میں
ب
برے دن مول کسے تے آون نہیں جدائیاں دے
کھلیاں زلفاں گلیاں دے وچ پھردی وانگ سودائیاں دے
جھلی کملی دنیا آکھے مہنے اکھیاں لائیاں دے
آکھ حنیف نہ ملیا ماہی، ڈاہڈے پھٹ جدائیاں دے

ت
ترازو لگا ہوسی عملاں والے ترسن گے
نیک کمائیاں کرسن جیہڑے، بھج جنت نوں وڑسن گے
جنہاں ہوسی خلق ستائی ، آن فرشتے پھڑسن گے
آکھ حنیف شفاعت ساڈی، آپ محمدؐ کرسن گے

ث
ثالث وچ پیار اساڈے ، صدق یقین صفائی اے
جان حوالے ، واگ وفا دی، سجناں ہتھ پھڑائی اے
ہو ہو عاجز نیوں لگایا، کیتی نہیں وڈیائی اے
یار حنیف نمانا روندا، سنجاں بے پروائی اے
ج
جہانوں اینویں جاسی جس نہ عشق کمایا اے
حسن بازار لگا اے دنیا، عشق سوداگر آیا اے
عشق مجاز حقیقی بھانویں، ہوندا نہیں پرایا اے
ویکھ حنیف عشق دے اندر، سب کجھ داء تے لایا اے

ح
حاصل کجھ نہ ہویا ہرگز پھر وی بازی جیتی اے
جیہڑی ہار محبت اندر، اوہ بنیاد پریتی اے
مزہ وصل دا دگنا ہویا، پیار پیالی پیتی اے
یار حنیف نہیں پچھے پلٹے، جند قربان چا کیتی اے

خ
خبر نہ دلبر مولے، ساہ لباں تے آیا اے
وقت نزع دے موت آسانی، چا دیدار کرایا اے
روز میثاق دے بول ’’بلا‘‘ دا وعدہ یار نبھایا اے
یار حنیف دی جان پرائی ، اینویں ایہہ وڈیایا اے

د
دکھاں دی دھرتی ایتھے، دکھ سکھ ساجھا جرنا ایں
رل کے کھانا، رل کے رہنا، رل...

اسلام میں سركاری اہلكاروں كا ضابطہ اخلاق

The purpose of this research paper was to reflect the code of conduct of Hazrat Omar Farooq for civil servants. The material for this descriptive paper was taken from Sahah e Sittah. writings of the uncontroversial Muslim scholars, Historians, documents and original sources, like letters of Hazrat Omar Farooq to civil servants about the duties and responsibilities. After in depth analysis, in the light of intensive review of literature, suggestions and solutions are given to issues and problems which we are facing these days at national level. The paper concludes that most of the issues/ problem will be resolved as a result of adoption/ implementation of these golden principles already practices during period of Hazrat Omar (RTA).

A Case Study of Alternative Assessment Practices in a Ccac Graduate Primary Science Classroom, in a Community-Based School in Pakistan

It is believed by many educationists that the traditional assessment approaches are less effective in students' learning. The contemporary trend in education favours the practice of alternative forms of assessment for improved students' learning outcomes. However, in the context of Pakistan traditional forms of assessment are being practiced in the classroom, which is considered a hindrance towards the holistic development of the learner and for the overall quality education in the country. The main aim of this study was to explore a science teacher's purposes, practices, challenges and opportunities of implementation of alternative assessment, who has participated in AKU-IED's Certificate in Classroom Assessment Course to contribute towards the understanding of implementation of alternative assessments in developing country context and subsequently to improve students' learning. A qualitative case study approach was used to explore the research topic. A primary level science teacher of a community based high school in Karachi, Pakistan was the primary participant, while the vice principal, section head of primary, a science teacher and a group of students of the school, formed the secondary participants. The data was collected through classroom observation, interviews, document analysis, field notes and reflective journal. The study revealed that, the research participant used alternative assessment for variety of purposes such as to enhance students' learning, providing feedback and grading. Questioning was practiced for getting students' attention and promoting thinking skills. Self assessment was used for giving students responsibility of their own learning and performance assessment was practiced to provide students opportunities to demonstrate variety of skills. The opportunities for implementing alternative assessment included teacher's professional development, which developed her basic understanding and skills for planning and implementation of alternative assessment. The skills included developing open ended questions, designing objectives based assessment tasks, setting clear learning targets and criteria which are aligned with her instructions and the provision of clear criteria and rubric based instructions to the students. Moreover, the features such as supportive school culture and autonomy for teacher to develop own syllabus, were seen to facilitate the process of alternative assessment in the classroom. The alternative assessment practices were also seen to facilitate student learning through their involvement in the activities, motivations towards their work and responsibility of their own learning. However, the study also revealed limited use of alternative assessment, such as lack of provision of opportunity for students to improve their work based on timely feedback. The challenges were limited