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Enhancing Bioavailable Phosphorous in Soil Through Sulfur Oxidation by Thiobacillus

Thesis Info

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Author

Ullah, Irfan

Program

PhD

Institute

Pir Mehr Ali Shah Arid Agriculture University

City

Rawalpindi

Province

Punjab

Country

Pakistan

Thesis Completing Year

2013

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2454/1/2848S.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726061781

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Phosphorus (P) fertilizer use efficiency is only 15 % in calcareous alkaline soils as in Pakistan. Sulfur oxidizing bacteria (SOB) especially Thiobacillus spp. solubilize the unavailable P in soil by synthesizing sulfuric acid. This study was performed in three steps: (i) screening of SOB from different microbial ecologies, (ii) use of different SOB with S levels for enhancing bio-available P, and (iii) effect of SOB along with P fertilizer and S on plant growth and yield. Sulfur oxidizing bacteria were isolated, screened, identified and the most efficient SOB were found as the genus Thiobacillus in sulfur based ecologies such as industrial wastewater, sewerage water and sulfur mud. These SOB isolates were IW1, SW2, SS1, IW13, IW14, IW16 and SM1 which reduced the pH of different media (thiosulphate, tricalcium phosphate and rock phosphate) in 07 to 40 days after inoculation. Four Thiobacillus isolates viz., IW16, SW2, IW1 and IW14 were inoculated in soil with three S levels 50, 37.5 and 25 mg kg-1. Thiobacillus spp. IW16 and SW2 reduced soil pH with 50 mg S kg-1 from 7.90 to 7.12 and 7.28 respectively. Similarly, Thiobacillus strain IW16 in combination with 50 mg S kg-1 reduced CaCO3 contents from 7.14 to 6.93 % and solubilized Ca8-P and Ca10- P. Increase in the concentration of sparingly soluble Ca2-P (20.33 mg kg-1) and xvii bioavailable P contents (net increase of 22.26 mg kg-1) were also recorded as a result of P solubilization phenomenon. Lastly field experiments were conducted on two permanent lay outs (plot size 3 m × 3 m) at two different places growing for rice-wheat and maize-maize crops. The best Thiobacillus isolates (IW16 and SW2) along with 100 kg S ha-1 were inoculated in combination with two doses of P fertilizer viz., 45 and 90 kg P2O5 ha . Significant increase in the concentration of bio-available P in soil was recorded through bacterial S oxidation in both experiments. Growth and yield parameters of the tested crops (rice, wheat and maize) exhibited positive significant correlation with P solubilization through S oxidation by Thiobacilli with the highest values by strain IW16 along with 100 kg S ha-1. Interaction between Thiobacillus spp. and S was highly significant in enhancing the growth and yield of crops. Treatment of soil with Thiobacilli and S was the best practice for enhancing bioavailability of P already present as fixed P in huge quantity.
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علامہ طنطاوی جوہری

آہ!علامہ طنطاوی جوہرؔی
گذشتہ ماہ فروری میں مصر اور ہندوستان کے دوبڑے عالموں نے وفات پائی۔ مصر کے مشہور عالم علامہ طنطاوی جوہری عہد حاضر میں اُن مسلمانوں کے خواب کی سچی تعبیر تھے جو علوم جدیدہ کی خیرہ کن جگمگاہٹ سے مرعوب ہوکرایسے جید عالم کی تلاش میں رہتے تھے جو ان علوم کے مقابلہ میں اسلامی و قرآنی حقائق کی فوقیت اس تحقیقی انداز میں ثابت کرسکے کہ بڑے سے بڑے ماہر علوم کوبھی مجال انکار باقی نہ رہے۔وہ جس طرح دینیات اورعلوم قرآن وحدیث میں یگانۂ روزگار تھے اُسی طرح اُن کو جدید علوم، علم نباتات، علم حیوانات، فلسفہ، سائنس، تاریخ اورعلم ہیٔت و طبقات ِارض میں بھی بڑی دستگاہ تھی۔ پھران سب فضیلتوں پر مستزاد یہ کہ اُن کا ذوق نہایت مستقیم اور طبیعت انتہا درجہ سلیم تھی۔ان کی قوت فیصلہ درست اورملکۂ تنقید صائب تھا۔بے شبہ اُنھوں نے اس دور میں وہی کام کیا جوامام غزالی اورابن رشد نے فلسفۂ یونان کے مقابلہ میں اسلام کی حفاظت و صیانت کے لیے انجام دیا۔ان دونوں بزرگوں نے فلسفۂ یونان کا عمیق نظر سے مطالعہ کیااوراُس میں وہ کمال پیداکیا کہ فلسفہ کے ایک ایک جزیہ اورمسئلہ پرحاوی ہوگئے۔پھر فلسفہ کے جو اصول حق بجانب تھے اُن کو اسلام کی تعلیمات پرمنطبق کیا اور جو لغو و لاطائل باتیں تھیں اُن کا تارو پود ’’تہافت الفلاسفہ‘‘لکھ کر اس تحقیق وکمال دیدہ وری کے ساتھ کھولا کہ فلسفہ کا خلعتِ زریں ایک گداگر عقل و خرد کی گڈڑی میں تبدیل ہوکررہ گیا۔
علامہ طنطاوی ۱۲۸۷ھ میں پیداہوئے۔علوم مروجہ کی تحصیل وتکمیل کے بعد قاہرہ کے مدرسہ دارالعلوم میں استاد مقرر ہوگئے۔درس وتدریس کے شغل کے ساتھ اُنھوں نے تصنیف وتالیف کامشغلہ بھی جاری رکھا اورمتعدد کتابیں تصنیف کیں جن میں چند اہم کتابیں یہ ہیں:(۱)الارواح،(۲)اصل العالم،(۳)این الانسان،(۴)التاج المرصع بجواھرالقرآن والعلوم،(۵)جمال العالم،(۶)الزھرۃ،(۷)السرالعجیب فی حکمۃ تعدد ازواج...

حکم الانتفاع بالمرھون عند الفقهاء: دراسة مقارنة

Islam is a complete code of life and dominant upon all the faculties of life. Along with creed faith and worship, Islam has emphasized on the affairs of daily life. In the connotation of affairs all the components of sale and purchase mentioned in detail. Accordingly, there is a provision if someone sell a thing to another and the buyer is not eligible to pay the price then there should be written a statement in the presence of two witnesses to pay the price in fix time. If there is neither witness nor the arrangement of writing, so there should be mortgaged something from the buyer. When the buyer pay a loan then the seller should return the mortgaged thing. The originality of mortgage is for the protection of loan not for profit. In present era the purpose of mortgage has changed and is done it for getting profit. Although some imams and Ahl-e-Zawaher provide the capacity for getting profit, so the Jamhoor and Ahnaf don’t allow anyone to get it. To change the original shape of mortgage is illegitimate and is considered as usury. If there is no custom in a certain territory and the man who offer loan does not intend to get profit then with the permission of the person who has received loan __provides the facility to get profit, However to avoid this practice is far better in shariah. In the article understudy the originality of mortgage and dissension among different Imams to get profit from it has been discussed. In the end a crucial statement has been mentioned.

Application of Environmental Friendly Practices for Tannery Sludge Reuse by Anaerobic Co-Digestion

Tanning industry in Pakistan has shown rapid growth in last few decades. However, environmental regulations are not strictly followed regarding the processing as well as discharge of effluents. Tannery wastewater and sludge have become a major source of water and soil pollution. This research study was designed to appraise the harmful impacts of untreated tannery wastewater along with proper management of sludge by utilizing green technologies. In this study, management of tannery sludge was done by utilizing environment friendly practices in four experimental phases. In first phase, the impacts of tannery wastewater on soil and selected plants were studied in comparison with a control group. Characterization of tannery wastewater showed that values of parameters like pH, biological oxygen demand, chemical oxygen demand, sulphate and chloride were higher as compared to National environmental quality standard. The concentration of Cr, Zn, Mn, Fe, Cu, Na, and K were higher in soil and plants (D. sanguinalis, S. cumini and E. camaldulensis) samples irrigated with tannery wastewater as compared to fresh water samples. It is concluded that the untreated tannery wastewater is not suitable for irrigation purpose. Hence, proper treatment of tannery wastewater is required before its usage for irrigation purpose. Biogas production through anaerobic co-digestion of waste activated tannery sludge (WATS) with biomasses (rice husk, cow dung and sewerage sludge) as co-substrates was investigated in second phase. The initial results of waste activated tannery sludge were best as compared to primary lagoon sludge, secondary lagoon sludge and tannery solid waste collected from Kasur tannery treatment plant. The waste activated tannery sludge was used in biogas experiments as co-substrate. Different proportions of waste activated tannery sludge and cow dung were used for biogas production. The cumulative biogas production in WATS (waste activated tannery sludge 100 %) was found 80 mL (0.10 mL/g TVS), WATSCD-1 (WATS 70 % and cow dung 30 %) that is 158 mL (0.20 mL/g TVS) and WATSCD-2 (WATS 50 % and cow dung 50 %) sample 195 mL (0.24 mL/g TVS). The overall results of WATSCD-2 combination were best. To optimize biogas production ultrasonic and chemical pre-treatment were applied through series of experiments on different combination of WATSCD-2. Seven combinations of WATSCD-2 with sewerage sludge and rice husk were ii prepared and designated as CDCT-1 to CDCT-7, treated chemically. Similarly, seven proportions CDUT-1 to CDUT-7 were treated ultrasonically. Biogas production results of ultrasonically treated sludge combinations (CDUT-1 to CDUT-7) were better as compared to chemically treated sludge combinations (CDCT-1 to CDCT-7). The optimum results were obtained in ultrasonically treated combination CDUT-7 (202 mL, 9.25 mL/g TVS) as it contains maximum biomass concentration as compared to CDUT-1 to CDUT-6. In third phase, series of experiments were conducted to optimize the parameters like sulfur, ash and gross heating value (GHV) by preparing different sludge combinations with biomasses. These combinations were prepared by using primary sludge, secondary sludge and WATSCD-2 with coal and rice husk on weight percent basis. The higher GHV results were found in WATSCD-2 combinations as compared to both primary and secondary sludge combinations. The WATSC-2 combination (WATSCD-2 50 % and coal 50 %) have lower sulfur and ash contents along with high GHV as compared to primary and secondary sludge combinations. So, this combination has better potential to use as refuse derived fuel for energy recovery. The statistical analysis (one-way ANOVA) was found significant for ash, sulfur and GHV for different sludge combinations with p < 0.05. It is concluded that sludge is a very valuable fuel like traditional biomass. However, efforts should be exercised to overcome the problem of drying and emissions from sludge. In last phase, compost was prepared from different combinations of WATSCD-2 with different co-substrates. These combinations were designated as WATSCD-SR (mixture of WATSCD-2, sewerage sludge and rice husk), WATSCD-RL (mixture of WATSCD-2, rice husk and leaves), WATSCD-SL (mixture of WATSCD-2, sewerage sludge and leaves) at laboratory scale by using 1:1:1 ratio of each component. The WATS-RL combination showed better results of nitrogen (2.9 ± 0.38 %), phosphorous (0.038 ± 0.01 %), potassium (0.07 ± 0.01 %), organic matter (57.55 ± 6.35 %) and C/N ratio (18.05 ± 1.93) as compared to WATS-SR and WATS-SL. Environmental regulations are getting more restricted related to landfilling of biodegradable waste. The solution of these problems is to use of biodegradable portion for biogas production and residual tannery sludge as bio-solid (composting) or thermal utilization for energy recovery.