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Home > Enviro Onmenta Al Impa Act and Risk As Sessmen Nt of Genet Tically Modifie Ed Pean Nut Arac Chis Hypog Gea L.

Enviro Onmenta Al Impa Act and Risk As Sessmen Nt of Genet Tically Modifie Ed Pean Nut Arac Chis Hypog Gea L.

Thesis Info

Access Option

External Link

Author

Robab, Um -E-

Program

PhD

Institute

Pir Mehr Ali Shah Arid Agriculture University

City

Rawalpindi

Province

Punjab

Country

Pakistan

Thesis Completing Year

2014

Thesis Completion Status

Completed

Subject

Botany

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1759

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726068790

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The development of transgenic crops is extensively changing the landscape in agricultural production and at the same time raising world-wide debate regarding the safety as well as regulatory process of genetically modified food and feed crops. Genetically engineered peanut crop having rcg3 and bar gene (resistant to tikka disease and tolerant to herbicide) were subjected to risk assessment according to guidelines given by OECD. In the first year experiment was conducted in the green house at National Agricultural Research Centre, Islamabad. In second year transgenic plants were grown along with their non transgenic counterparts at NARC , Islamabad, Groundnut Research Station, Attock (GRS) and Barani agricultural research Institute (BARI), Chakwal for evaluation of performance and biosafety concerns in different environmental conditions. No horizental or vertical gene flow was observed from the transgenic plants to non transgenic plants and soil microbial communities. Transgenic seeds were also found to be substantially equivalent with their non transgenic counterparts in terms of protein, fat , fatty acid and micronutrient contents in the seeds etc. Aflatoxin levels were also evaluated using HPLC in transgenic and non transgenic seeds and it was found that insertion of rcg3 gene significantly controlled the aflatoxins in transgenic seeds. As Peanut grains are directly consumed by end user, therefore crop was also evaluated for its allergy and toxicity potential by using rabbit as test animal. Genotoxicity and cytotoxicity among the test animals was studied using Comet and micronuclei assay respectively. The resulting images obtained from the Comet and micronuclei assay revealed no DNA damage and all lymphocytes appeared round with single nuclei. Genetically engineered strains of bacteria 12 Salmonella typhimurium also indicated no mutagenic potential of transgenic seeds. M crops showed significant increase in the yield and yield parameters and this increase was maximum in the plants having rcg 3 gene mainly through the control of fungus disease. Therefore, it can be concluded that although in the current study GM peanut had not exerted any toxic effect in the specific conditions of the experiment. More detailed and through investigation is required in this regard by designing some multigenerational studies keeping in view the use of peanut as a food and feed crop.
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امینِ حزیں

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تیسرا مجموعۂ کلام ’’سرودِ سرمدی‘‘ بھی الفیصل ناشران و تاجران ادارے نے شائع کیا۔ امینِ حزیں کی شاعری کے آٹھ مسودے ابھی تک شائع نہیں ہو سکے۔

یہ آٹھوں مسودے ان کے عزیز و اقارب کے پاس موجود ہیں۔ امینِ حزیں کے ہزاروں کی تعداد میں مشاہیر کے نام خطوط بھی محفوظ ہیں۔ اردو ادب کے محققین کے لیے یہ شعری و نثری فن پارے قیمتی سرمایہ ہیں۔

امینِ حزیں ایک مشاق اور قادر الکلام سخن ور تھے۔ انہوں نے تقریباً ہر صنفِ سخن میں طبع آزمائی کی ہے۔ انہیں اردو‘ عربی‘ ہندی ‘ سنسکرت‘ انگریزی‘ پشتو اور...

شخصيت انسانى پر شہادتين كے قرآنى اثرات

Allah Almighty sent mankind in this world for His worship only. This is high right on humans those who keep the faith and belief in the one Supreme God and obey His orders. When they followed satanic powers, they forgot the eternal lesson of Tawheed (Oneness of Allah) and engaged Shirk (polytheism). It is the dogma of Oneness of Allah with which mankind gets tranquility and peacefulness in society and becomesconstructive and valuable for humanity. And when human left his belief on Allah, they germinate their negative role and made the society despicable. In this scenario Allah Almighty sends His prophets to those nations which create mess and fill the society with clutter. At that time prophets play their role as a bounty of Allah on human race. They purify people by their teachings granted by Allah and focus their attention to the eternal massage of God. So Eman (faith) on prophet hood considered as essential as belief on Oneness of Allah. Both these beliefs; Tawheed and Risallah effect human life prominently in such a way that man redevelops his whole personality and becomes a required sound character of Eman. Then worldly contamination cannot harm human being's decency and he becomes a felicitous character of vigorous society.

Supervising Control Mechanism for Uavs to Enhance Robustness

Small UAVs, due to their easy handling and lesser logistic support requirements are attracting lot of interest for applications ranging from military to commercial, and even for scientific data collection. In present day scenario, they are mostly used to conduct surveillance, reconnaissance, search and rescue missions for military and civilian outfits. In order to complete the assigned tasks they are usually equipped with surveillance cameras. Unlike their larger counterparts, smaller UAVs by virtue of their size cannot carry sophisticated gyro stabilized cameras. It therefore becomes important to address this shortcoming, in terms of non availability of gyro stabilization by ensuring a smoother flight even in turbulent weather to receive a stable and good quality video from UAV. Growing interest in small UAVs has motivated many equipment manufacturers to develop Off The Shelf autopilots. Being cost effective, simple, flexible and reliable, PID based autopilots are generally preferred for the purpose. Besides number of advantages, PID controllers have a limitation that they sometimes require retuning of the gains when subjected to external disturbance. UAVs perform satisfactorily if the atmospheric conditions do not change much. However, if the UAV moves to a location where the atmospheric conditions change substantially, the PID gains may require readjustment / retuning, which is a tedious job and requires numerous UAV flights; otherwise performance degradations in terms of pitching and rolling oscillations are observed. The above premise formed the basis and source of motivation for this research. It was considered that instead of going for retuning, gain scheduling or replacing the existing, conveniently available PID controller with some intricate or complex controller, a simpler, realizable and effective scheme should be adopted. The basic idea of the proposed scheme was derived from a situation in which a boy sometimes requires a bit of extra hand from his father sitting beside him, while driving through a bumpy and wavy patch of road. Father closely monitors the driving performance of his son on bumpy road and when he thinks that slight help in terms of applying an extra force by holding the steering can improve the capability of his son to negotiate the bad patch, he does so. After passing through bumpy part of road when father senses that the extra effort is no more required, he gradually removes it and his son takes over the car back again as a solo driver. Following the same analogy, the idea was conceived to propose a supervising mechanism which can act like a human pilot and can be augmented with an existing control system of a UAV for improving its performance in presence of atmospheric disturbances without going into exercise of retuning the controller gains. Our supervisory mechanism is composed of two modules, “observer module” and “correction generator module”. We developed a human thinking like logic for observer module so that it keeps monitoring the status of flight through specified inputs and outputs of the system and instructs the correction generator module to augment main controller by adding compensation commands when required i.e. in case undesired attitude error is observed. The second module for correction generation is based on Fuzzy rules, where rule set was developed using input from a UAV pilot. Effort is made to maintain a logical layout of this thesis so that the reader can be familiarized with the theoretical concepts and analysis tools related to UAVs. A small UAV with already published aerodynamic data is taken as a reference bird. In chapter two and three of this thesis detailed explanation about the formulation of its nonlinear mathematical modeling, linearization process to achieve decoupling and simplicity, conducting static and dynamic stability analysis, criteria for flying qualities assessment is given. Designing of PID controllers for attitude, altitude and heading control using linearized UAV models is covered in chapter four. Performance of designed controller is also validated by using nonlinear UAV model. Atmospheric disturbances such as wind shear and turbulence significantly influence the attitude of UAVs. Rotary moments, rolling moments, vertical and lateral wind gusts generated due to moderate to severe turbulence are simulated as atmospheric disturbance. Pitch and roll response in presence of these disturbances are presented. In chapter five the functionality of proposed scheme is discussed in detail and simulations are run to demonstrate improved pitch and roll response under atmospheric disturbance by augmenting the supervisory mechanism. Simulation results showing significant reduction in attitude errors with slight increase in control effort after augmenting the supervisory mechanism are presented. Simulations are run using linear as well as nonlinear model of UAV. Comparison of results obtained from standalone PID controller and augmented system is presented to validate the efficacy of proposed scheme Keywords; UAV Modeling, Stability analysis of UAVs, UAV autopilots, Disturbance rejection in UAVs