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Home > Epidemiology of Foot and Mouth Disease in Sheep and Goats in Punjab

Epidemiology of Foot and Mouth Disease in Sheep and Goats in Punjab

Thesis Info

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Author

Rehman, Saif-Ur-

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2013

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1176

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726079553

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Foot and mouth disease (FMD) is a highly contagious disease of ruminants that causes huge economic losses around the globe. However, the prevalence of FMDV in small ruminants has been overlooked in Pakistan. The present study was designed to determine the sero-prevalence of FMD in sheep and goats in Punjab and to identify the prevalent serotypes of virus in sheep and goats in the study area. The efficacy of different commercial vaccines of FMD in sheep and goats under field conditions was also compared. The current study was completed in three phases. In 1 st phase, a seroepidemiological study was conducted in Chakwal, Faisalabad and Khanewal districts of Punjab, Pakistan to determine the prevalence of FMD in sheep and goats. A total 1200 serum samples were collected from sheep (n= 180) and goats (n= 920) and were subjected to 3ABC Non Structural Protein Enzyme Linked Immunosorbent Assay for detection of antibodies against non-structural proteins of FMD virus. In 2 nd phase of study, samples collected from clinical cases were confirmed for FMD virus using RT- PCR and serotyping of virus was done using indirect sandwich ELISA. In 3 rd phase of study, post-vaccine antibody titers were determined in sheep and goats using Indirect Haemagglutination Test (IHAT). One bivalent imported vaccine (Aftebin) and two trivalent vaccines (one imported vaccine, Aftovaxpur and one local vaccine, VRI-FMD) were tested. Results of 1 st phase of study showed that the overall seroprevalence of FMD in sheep and goats was 21 % (n=252) while 19.44 % (n=35) and 21.27 % (n=217) prevalence was recorded in sheep and goats respectively. Highest seroprevalence (32.5%) was observed in southern Punjab (Khanewal), followed by (25.75 %) central Punjab (Faisalabad) and the lowest seroprevalence (4.75 %) was detected in northern Punjab (Chakwal). There was no statistically significant difference in seroprevalence between sheep and goats. Among different risk factors tested, age and sex were found to be significantly associated with the prevalence of disease while pregnancy and herd type had no association with the prevalence of the disease. In 2 nd phase of study, a total of 4 outbreaks were reported during the study period and a total of 13 epithelial tissue samples were collected (10 from goats and 3 from sheep) from these outbreaks. Results with RT- PCR showed that 4 out of 13 field samples were FMD virus. All these 4 positive samples were taken from goats of district Khanewal. Indirect sandwich ELISA was applied to the samples for serotyping of FMD virus and all 4 positive samples were confirmed as IXserotype “O” of FMD virus. Results of 3 rd phase of study showed that highest Geometric Mean Titer against all 3 serotypes of virus was recorded in animals that were injected aftovaxpur followed by aftebin and VRI-FMD vaccine. The current study illustrate that FMD is highly prevalent in sheep and goats in Punjab. Therefore, a broader study is needed to ascertain the countrywide prevalence of FMD in small ruminants.
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مولانا محمد اسحاق سندیلوی ندوی

مولانا محمد اسحاق سندیلوی ندوی مرحوم
پاکستان سے یہ افسوسناک خبر بہت تاخیر سے ملی کہ مولانا محمد اسحق سندیلوی ندوی کا نوے ۹۰ سال کی عمر میں انتقال ہوگیا۔ اناﷲ وانا الیہ راجعون۔
مولانا کی تعلیم مدرسہ فرقانیہ اور دارالعلوم ندوۃالعلماء میں ہوئی، عرصہ تک وہ دارالعلوم میں درس و تدریس کے فرائض انجام دیتے رہے، جب مولانا محمد اویس نگرامی ندوی، ندوہ کے شیخ التفسیر تھے اس وقت مولانا سندیلوی شیخ الحدیث تھے اور ان دونوں جید اساتذۂ فن کی موجودگی ندوہ میں قران السعدین کا منظر پیش کرتی تھی، وہ ندوہ کے مہتمم بھی رہے اور وہاں کی مجلس اشاعت اور تحقیقات شرعیہ کے ناظم بھی۔
درس و تدریس کے ساتھ ان کا تصنیفی ذوق اور تحریری مذاق اعلیٰ درجہ کا تھا، تاریخ وفقہ اسلامی پر ان کی نظر وسیع و عمیق تھی، ۱۹۴۷؁ء سے قبل مسلم لیگ کے ذمہ داروں کو خیال ہوا کہ متوقع اسلامی حکومت کا ایک قانون اساسی، اسلامی تعلیمات کی روشنی میں مرتب کیا جائے تو اس کے لیے یو پی مسلم لیگ نے نظام اسلامی کے نام سے ایک مجلس کی تشکیل کی جس کے ارکان میں مولانا سید سلیمان ندوی، مولانا عبدالماجد دریا بادی، مولانا سید ابوالاعلیٰ مودودی اور مولانا آزاد سبحانی جیسے جید علماء شامل تھے، مجلس کے روح رواں حضرت سید صاحب کی جو ہر شناس نظر اس اسلامی قانون کے خاکہ و دستور کی ترتیب و تیاری کے لیے مولانا اسحق سندیلوی ہی پررکی، جنھوں نے بڑی خوش اسلوبی سے ایک ضخیم کتاب تیار کی جو بعد میں دارالمصنفین سے اسلام کا سیاسی نظام، کے نام سے شایع ہوکر مقبول ہوئی اس میں انہوں نے نظریہ خلافت، قانون، حکومت، خلیفہ، مجلس تشریعی، رعایا، بیت المال، افتا، احتساب، حرب و دفاع، صوبائی حکومتیں، خارجی معاملات پر دور جدید کے سیاق و سباق میں فاضلانہ بحث...

اردو تراجم و تفاسیر میں تفسیر مرادیہ کا مقام و مرتبہ

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Image Clustering Using Novel Local and Global Exponential Discriminant Models

Image clustering deals with the optimal partitioning of images into different groups. Using linear discriminant analysis (LDA) criterion, optimal partitioning of images is obtained by maximizing the ratio of between-class scatter matrix (Sb) to within-class scatter matrix (Sw). In global learning based clustering models, scatter matrices (Sb and Sw) were evaluated on whole image datasets. Owing to which, nonlinear manifold in image datasets may not be effectively handled. For manifold learning, local neighborhood information in data objects were utilized in local learning based clustering models. Further, for high-dimensional data, Sw is singular which corresponds to under-sampled or small-sample-size (SSS) problem of LDA. Owing to which, almost all global learning and local learning based clustering models are based on regularized discriminant analysis (RDA), a variant of LDA. In RDA, the singularity problem of Sw was solved by perturbing it with regularization parameter λ > 0. However, tuning for optimal value of parameter λ is required. Further, for optimal clustering performance in existing state-of-the-art local learning based clustering models, one has to tune a number of clustering parameters from a large candidate set. In this thesis, we propose a novel local learning based image clustering model. Our proposed clustering model is inspired from exponential discriminant analysis (EDA). EDA is another variant of LDA in which SSS problem of LDA was handled using matrix exponential properties. Owing to which, EDA is less parameterized as compared with RDA. Number of nearest neighbor images k is the only clustering parameter in our proposed clustering model as compared with existing state-of-the-art local learning based clustering models. Image clustering performances on 12 benchmark image datasets are comparable over near competitor RDA based image clustering model. Performances are comparable because no discriminant information of LDA is lost in EDA. However, well separated images may not be achieved at local level for image datasets that contain images with pose, illumination, or xiv occlusion variations. Owing to which, local learning based image clustering models may face limitations in such variations. For this problem, various clustering models were proposed in which both global learning and local learning approaches were utilized. However, almost all existing local and global learning based clustering models are based on RDA. Owing to which, tuning of clustering parameters is extensive in almost all state-of-the-art local and global learning based image clustering models. We propose novel local and global learning based image clustering models that are inspired from EDA. Our proposed image clustering models are less-parameterized and computationally efficient where image clustering performances are comparable with existing local and global learning based clustering models. However, performances of all state-of-the-art clustering models are not optimal for challenging image datasets that contain images with illumination and occlusion changes. We explore the challenges in image clustering problem. We show that variation from one image to another image in a class (within-class variation) of an image dataset may vary from nominal to significant due to images with different facial expressions, pose, illumination, or occlusion changes. Using pixel intensity values as image features, we obtain histogram of within-class variation for each image datasets. On the basis of histogram, we categorize image datasets as Gaussian-like or multimodal. We show that image clustering performances of state-of-the-art clustering models are optimal for Gaussian-like image datasets and it degrade significantly for multimodal image datasets. We achieved significant overall performance improvement on 13 benchmark image datasets by employing optimal image descriptors with our proposed clustering model. Our study shows that there is no direct correlation between image clustering performance and local neighborhood structure. However, image clustering performance has correlation with the distribution of within-class variation in image datasets.