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Home > Epidemiology of Principal Viral Respirtaory Pathogens in Pakistani Children and Molecular Characterization of Influenza A/H3n2 and Respiratory Syncytial Viruses

Epidemiology of Principal Viral Respirtaory Pathogens in Pakistani Children and Molecular Characterization of Influenza A/H3n2 and Respiratory Syncytial Viruses

Thesis Info

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Author

Bashir, Uzma

Program

PhD

Institute

National University of Sciences & Technology

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Virology & Immunology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9980/1/Uzma_Bashir_Virology_%26_Immunology_HSR_2017_NUST_25.05.2017.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726080344

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21 ABSTRACTBSTRACTBSTRACTBSTRACTBSTRACTBSTRACTBSTRACT Acute respiratory infections (ARI) are associated with high morbidity and mortality rates at the global level. Approximately 20% of the deaths attributable to pneumonia globally occur in Pakistan with nearly one half the infections among children younger than 5 years of age. The objectives of this study were to define the molecular epidemiology of respiratory viruses in Pakistani children and to identify viral genotypes causing respiratory infections. In this study, we analyzed 1941 respiratory samples collected over three winter seasons and tested for common respiratory pathogens using real time PCR. We found 76% prevalence rate for viral pathogens with Influenza and RSV predominant among 0.01-6 and 12-24 months age groups respectively. Fever and cough were identified as significant clinical parameters along with malnutrition and maternal breastfeeding. Furthermore, phylogenetic analysis of representative Influenza A/H3N2 viruses showed that recent viruses belonged to subgroup 3C.3 and carried important amino acid substitutions in the H3 hemagglutinin, while no substitutions attributable to neuraminidase resistance were detected. We genotyped the Respiratory Syncytial viruses subtypes and analyzed the two surface glycoproteins G and F of over100 isolates. All RSV-A strains carried 297 amino acids long G protein and clustered in the NA1/GA2 genotype. We also reported a novel RSV B genotype BA-13 and two other genotype variants (BA-9 and BA-10). We analysed the fusion protein F in RSV A isolates at the binding sites for the monoclonal antibodies Palivizumab and Motavizumab specifically and found that the N276S substitution in over 90% of RSVA and all RSVB viruses. This work highlights the need for respiratory disease surveillance as it will support the development of appropriate public health surveillance programs to inform health planning.
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مولانا قاضی محمد معین اﷲ ندوی

مولانا قاضی محمد معین اﷲ ندوی
افسوس ہے کہ گزشتہ مہینے دارالعلوم ندوۃ العلماء کے نائب ناظم مولانا قاضی معین اﷲ ندوی اپنے وطن اندور میں انتقال فرماگئے، اناﷲ وانا الیہ راجعون۔
مولانا سید ابوالحسن علی ندوی کی سربراہی اور سرپرستی میں ان کے معاصرین کے ندوے جانے کے بعد اس کے علمی، تعلیمی اور انتظامی کاموں کو سنبھالنے کے لیے جو جماعت آگے بڑھی ان میں مولانا قاضی معین اﷲ ندوی کا نام زیادہ ممتاز ہے، ندوہ کے تعلیمی مراحل طے کرنے کے بعد وہ یہیں استاذ مقرر کئے گئے۔ اسی زمانے میں مولانا سید ابوالحسن علی ندوی بلاد اسلامیہ کی سیاحت کے لیے نکلے تو ان کے رفقائے سفر میں مولانا معین اﷲ صاحب بھی تھے۔ اس سفر کا انہیں فائدہ ہوا۔ چنانچہ جب ندوہ کے ذمہ داروں کو قدیم نظام تعلیم میں داخل عربی صرف و نحو اور ادب کی کتابوں کے نقائص کا شدت سے احساس ہوا تو انہوں نے نحو و صرف کی فارسی کتب کے بجائے اردو میں سہل کتابوں کی اشاعت کا پروگرام بنایا، اس کے لیے ان کی نظر دارالعلوم کے ان معلمین پر پڑی جو ابتدائی درجوں میں زبان و صرف و نحو کی تعلیم میں مشغول تھے، اس کے علاوہ مصر میں عربی زبان و ادب کی ترقی و اشاعت کے لیے اس کے اصول و قواعد کی از سر نو تدوین اور طرزِ تعلیم میں اصلاح و تجدید کی جو کوششیں ہورہی تھیں اس سے بھی یہ لوگ واقف تھے اس بنا پر ان سے مبتدیوں کی مشق و تمرین کے لیے نحو و صرف اور ادب و انشا کی کتابیں لکھنے کی فرمایش کی گئی۔ مولانا معین اﷲ صاحب نے اس سلسلے کی تکمیل میں تمرین الصرف لکھ کر ہاتھ بٹایا جو جدید طرز پر صرف کی ایک مشقی کتاب ہے۔
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