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Home > Epidynamics and Molecular Characterization of Peste Des Petits Ruminants Virus

Epidynamics and Molecular Characterization of Peste Des Petits Ruminants Virus

Thesis Info

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Author

Muhammad Abu Bakar

Program

PhD

Institute

The University of Agriculture

City

Peshawar

Province

KPK

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Animal Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/13253/1/Final%20Thesis-%202017_for%20Turnitin.doc

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726086639

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Peste des Petits Ruminants (PPR) is a highly fatal and economically devastating disease of sheep and goats. Present study was designed to have an insight into the molecular epidemiology of PPR in the country and to investigate the persistence and transmission of PPR virus using molecular tools under field conditions. A total of eighty-four PPR outbreaks were investigated during the course of this study (2010 to 2013). The highest number of outbreaks was reported from Punjab province followed by Sindh and KPK (Khyber Pakhtunkha). Overall, disease affected all three age groups of sheep and goats but the younger animals were more severely affected with a morbidity rate of 37.19%. The mortality and case fatality rates were also higher in young which were 46.86% and 17.39%, respectively. The results of the phylogenetic trees indicated that all Pakistani PPRV strains, regardless of the gene used either F or N, were clustered in lineage IV which is the most prominent and prevalent lineage of Asia. A total of 19575 serum samples from sheep and goats from all the provinces/ regions of the country) were included in this study to determine the sero-epidemiology of PPRV in Pakistan. Province-wise laboratory results are summarized in table 4.10. In total, it gave a 27.53 percent of the samples were positive for PPR antibodies. To understand the persistence and transmission dynamics of PPRV, one field outbreak was extensively investigated. PPR viral antigen was detected in fecal matter for one month following vaccination. In contrast the unvaccinated animals shed virus antigen in fecal material for up to two months following outbreak resolution. In conclusion, study is indicative of PPR endemic state in the country and the role of disease persistence, production systems of small ruminants as well as animal movements are key factors in the disease transmission and its endemicity.
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پروفیسر اسنوک ہرخر دنئے

پرنس کائتانی ؍ پروفیسر گویدی ؍ پروفیسر اسنوک ہرخردنئے
امسال مرحوم مارما ڈیوک پکتھال کے علاوہ ن کو ہم سب جانتے تھے، کئی نامور مستشرقین نے وفات پائی، اٹلی کے پرنس کائتانی اور پروفیسر گویدی اور لائڈن کے پروفیسر اسنوک ہر خردنئے نے امسال ہماری دنیا کو الوداع کیا، پرنس کائتانی تاریخ اسلام کے عالم اور گویدی عربوں کے ریاضیات اور جغرافیہ کے ماہر اور اسنوک ہر خردنئے ’محمڈنزم‘ نامی کتاب کے مصنف ہیں جس کو انھوں نے خطبہ کی صورت میں امریکہ کی ’’مجلسِ تاریخِ مذاہب‘‘ میں پیش کیا تھا اور بھی دوسری کتابیں اور مضامین ان کے قلم سے نکلے تھے۔ (سید سلیمان ندوی، اکتوبر ۱۹۳۶ء)

فقہ اسلامی میں مقاصد شریعت کے مدارج

Al-Maqasid (the purposes) is a guide to Islam written by Imam Shatibi in his book "Al-Mowafaq'at". It covers purposes of Islamic faith, Zakat, pilgrimage etc. Maqasid al-Shari’ah is a system of values that could contribute to a desired and sound application of the Shari’ah. ” This concept has been employed as a legal hermeneutical tool in pre-modern Islamic law at least since 3 H. D. It is based on the idea that Islamic law is purposive in nature, that is, to mean that the law serves particular purposes (e. G, promoting people’s benefit and welfare and protecting them from harm) that are either explicitly present in or can be derived from the fountainheads of the sources of Islamic law, namely, the Quran & the Sunnah. Maqasid al-Sharia is also an umbrella term that includes many other concepts that have been closely linked to it in the premodern Islamic tradition, most notably the idea of public interests and unrestricted interests (al-Masalih al-Mursala), as well as other principles such as istihsan (juridical preference), istis’hab (presumption of continuity), and avoidance of mischief (all of which are considered to be directives in accordance with Allah’s will). Spiritual Principles include: the free right and duty to be aware of and to worship Allah and to search for ultimate truth and justice; the duty to respect the human person, known as the natural principle of personalism; the duty to respect the coherent order of all creation, i. E. Ecology and environment; and the duty to respect human community based on the sacredness of each of its members.

Distribution and Management of Thrips Tabaci Lindeman Thysanoptera: Thripidae on Onion Allium Cepa in Selected Districts of Khyber Pakhtunkhwa Province, Pakistan

Onion (Allium cepa L.) is among the cash vegetable crop grown in Khyber Pakhtunkhwa Province, Pakistan. Onion thrips (Thrips tabaci) is the main problem on onions crop which results great losses. However infestation of this pest could be kept below economic injury level with the help of integrated pest management practices. In this connection, firstly field surveys were conducted in the selected onion growing districts of Khyber Pakhtunkhwa Province, Pakistan and recorded infestation/ population of T. tabaci. Highest numbers (57.59) nymphs plant-1 and (15.39) adults thrips plant-1 were recorded in district Swat while lowest (22.37) nymphs plant-1 and (9.69) adults thrips plant-1 were in district Peshawar (East. Part). Regarding the average number of both (nymphs and adults) plant-1, highest (36.49) thrips plant-1 were recorded in district Swat and lowest (16.03) thrips palnt-1 were in district Peshawar (East. Part). Eight onion cultivars (Ambika, Swat-1, Trichmir, Barkel, Macarena, Red ball, Granada Red and Sunset were assessed to determine the most tolerant cultivar against T. tabaci infestation. The results showed that T. tabaci infest all the cultivars more or less, however cultivar Swat-1 showed to be the most tolerant against T. tabaci infestation as well as proved to be a high yielding cultivar among the others. Highest plant height (64.35cm) was observed in cultivar Trichmir and lowest (38.40cm) was observed in Macarena. Highest (13.07) number of leaves plant-1 was recorded in Trichmir and lowest (7.66) was recorded in Granada red. Trichmir was (94.04%) the most succulent cultivar followed by Sunset (91.38%) and Swat-1 (86.49%), Ambika (87.21%) was least succulent cultivars. The maximum yield (18.56 tons ha-1) was obtained from Swat-1 and minimum was from Ambika (9.34 tons ha-1). The studies on Population dynamic revealed that T. tabaci remained a regular pest on onion during the onion growing seasons. The average peak populations were recorded (54.75 thrips plant-1) during the month of April. Population model using the meteorological data showed that average air temperature have a positive correlation with thrips population. The efficacy of Polytrin C® 440 EC (Profenofos + Cypermethrin) and three botanicals extracts (Azadirachta indica, Parthenium hysterophorus and Datura alba) and a combination of selected dose of synthetic insecticide with each botanical were assessed against T. tabaci on onion crop. All the botanicals extracts significantly control T. tabaci at various degrees over the control (untreated plots). Regarding the yield of onion bulbs, synthetic insecticide along with the combination of (half dose of insecticides along with half dose of each botanical) gave the highest yield followed by botanicals as compared xv to control (untreated plots). Results obtained from the laboratory experiments showed that, female western flower thrips Frankliniella occidentalis feed more and caused more feeding damage than that of males. Feeding damage caused by female F. occidentalis increased significantly as the numbers of female leaf-1 disc was increased. Azadirachta indica oil is significantly less effective than Conserve® (spinosad) in killing F. occidentalis. All the concentrations of neem oil significantly deterred F. occidentalis feeding when compared to controls treated with Tween or water. The botanicals Azadirachta indica, Parthenium hysterophorus and Datura alba extracts tested were as effective as Conserve® (spinosad) in killing F. occidentalis. All the botanicals extracts significantly reduced F. occidentalis feeding when compared to the water control. As expected given the very high mortality caused, the least feeding damage was found on spinosad treated leaf discs. Response of Orius laevigatus to chemical insecticide Conserve® (Spinosad) and botanicals extracts on the predation of T. tabaci showed that Conserve® caused negative response to both O. laevigatus and T. tabaci while there were no negative or mortality effects of botanicals extracts on O. laevigatus. The O. laevigatus consumed more T. tabaci in leaf discs treated with water followed by D. alba, A. indica and P. hysterophorus. Botanicals pesticides offer a safer alternative to using synthetic chemicals and have less or no impact on the environment and biological control agent of T. tabaci as well as botanicals can prevent pest resurgence. These findings encourage the use of botanicals extract which is pollution free approaches for onion thrips control.