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Estimates of Genetic Parameters in Rapeseed Using Diallel Analysis

Thesis Info

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Author

Khan, Wajid

Program

PhD

Institute

The University of Agriculture

City

Peshawar

Province

KPK

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Agricultural Technology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12112/1/Wajid%20Khan_PBG_2019_AUP_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726090565

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Pakistan is deficient in edible oils and for its domestic requirement it mainly relies on heavy import which costs more than two billion US dollar each year. This situation can be overcome by developing new high yielding oilseed genotypes. Rapeseed/ mustards is the second largest contributors in domestic edible oil production and if given due attention could help to reduce the burden of heavy imports. The present study aimed to estimate genetic parameters of morphological, yield and the seed biochemical traits in rapeseed. For the study, rapeseed genotypes namely AUP-01, AUP-05, AUP-07, AUP-08, AUP-10, AUP-13, AUP-I8 and AUP-2I were selected and hybridized in eight by eight diallel method to develop the FI crosses during 2014/15. In 2015/16 cropping season, evaluation of 56 FI hybrids along with parents were performed at AUP-Peshawar. Different statistical approaches were used to study genetic varriability, hetreosis, combining ability, gene action and h2 for days to flowering, maturity, primary branchs per plant, plant hieght, pods main receme-1, pod length, pods per plant, seed per pod, thousand-grain weight, seeds yield per plant and quality parameters including percent oil, percent protein, percent oleic acid, percent linolenic acid, glucosinolate and the percent erucic acid. The means square exhibited significant variation among parental lines and FI progenies signifying the presence of considerable difference for all the studied parameters among genotypes. Mean performance revealed parental genotype AUP-2I as best parent as it performed better for days to maturity (167.0 days), pods per plant (335.3), thousand grain weight (7.1 g), seeds yield (30.6 g), protein content (25.9%) and glucosinolate content (53.7%). However, AUP-07, AUP-13 and AUP-I8 were also found better for other traits. Among FI hybrids, AUP-10 × AUP-I8 showed maximum seeds yield (36.5 g) and moderate days to maturity (177). Desirable signaficantnegitive best parent and the comercial heterotic effects was detected in 19 and 12 hybrids for the days to flowers, 17 and 13 hybrids for maturity, 13 and 10 hybrids for linolenic acid, 39 and 6 hybrids for glucosinolates, and 40 and 37 hybrids for the erucic acid contents, respectively. Desired signaficant positive best parent and commercial heterosis were estimated for pods per plantin 16 and 17 FI hybrids, for thousand grain weight in 11 and 05 FI hybrids, and for seeds yield per plant in I8 and 01 FI hybrids, respectively. For oil and protein content, 13 and 19 and 12 and 11 crossese showed best parent and commercial heterosis, respectively. Current study revealed, AUP-05 × AUP-01, AUP-10 × AUP-I8, AUP-01 × AUP-07, AUP-2I × AUP-13 as well as AUP-I8 × AUP-10 as top ranking hybrids for important traits. SCA, GCA and RCA analysis applying Griffing Model-II, Method-I approach revealed highly significant general (GCA), specific (SCA) and reciprocal combining ability (RCA) effects for the studied traits except days to flowering and maturity. The Griffing analysis identified parental genotype AUP-05 best for pods length; thousand grain weight and protein contents. AUP-2I was best for maturity whereas AUP-I8 was best for pods per plants, seeds yield per plant, oil, glucosinolate and oleic acid. As per SCA effects, FI hybrids AUP-01 × AUP-05 was best for maturity, AUP-10 × AUP-I8 for seeds yield per plant, thousand grain weight, and erucic content, AUP -01 × AUP -07 for the oil contents, AUP-05 × AUP-08 for protein, AUP-05 × AUP-13 for glucosinolate content and oleic contents. Desirable Reciprocal effect for maturity and seeds yield per plant were revealed by the cross AUP-10 × AUP-05 and AUP-07 × AUP-01, repectively. However, desired RCA values for biochemical parameters were presented for AUP-07 × AUP-05, AUP-08 × AUP-01 and AUP-2I × AUP-01. The estimated variances due to general (σ2GCA) and specific combining ability (σ2SCA) and the predictability ratio being less than one specified the significant role of the non adittive genes for the studied traits in rapeseeds genotype. The scaling test (t2 test and regression analysis) for genetic analysis in FI generation proved partial adequate of edditive and dominance model for flowerings and maturaties, plant hieght, pods main shoot-1, pods per plant, pod length, seed per pod, thousand-seed weight, protein, erucic acid and oleic acid content whereas model for remaining studied traites was fully adequate. Hayman diallel analysis showed the signaficant role of both adittive and dominance genetic effects in the expression of important traites in rapeseed. Further, higher estimates of H1 and H2 (dominance) over D (adittive variances), showed prime position of non adittive genetic effect for the most traits. Environmental component was also significant specifying its role in the manifestation of the studied traites. The scattering of the genotypes on Wr/Vr graph showed the presence of genetic varriability among genotypes for the studied traits. The broad sence h2 values was medium to high whereas, narrow sence estimates was low, signifying greater role of dominant genes and the effectiveness of selection in later segregating generations for improving these parameters. From means and GCA, current studies identified AUP-05, AUP-I8 and AUP-2I, were found best (high) general combiners. These parental lines in combination with other lines (low) performed better hence and produced best crosses. On the basis of means, hetreosis and SCA/RCA, FI crosses (AUP-05 × AUP-01, AUP-10 × AUP-18, AUP-18 × AUP-10, AUP-05 × AUP-13, AUP-01 × AUP-07, AUP-05 × AUP-08, AUP-07 × AUP-01, AUP-2I × AUP-13 and AUP-13 × AUP-2I) were found best for important traits showing the involvement of high into high, high into low, low into low combinations in production of new hybrids. These parents and crosses could be an asset for rapeseed breeding programs. The information generated from this study is useful for breeders to develop hybrids/varieties with better seeds yield and oil quality traits through hetereosis breeding.
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جنرل محمد ضیاء الحق[صدرِ پاکستان]

جنرل محمد ضیاء الحق
اسلامی جمہوریہ پاکستان کے صدر جنرل محمد ضیاء الحق اوران کے ہمراہ کئی اعلیٰ فوجی آفیسر ۱۷/اگست کوبہاول پور کے قریب ایک فضائی حادثے کاشکار ہوکر جاں بحق ہوگئے۔ اناﷲ وانا الیہ راجعون۔
پرواز کے چند لمحے کے بعد ہی طیارہ میں زبردست دھماکہ ہوا اورمرحوم کا صدارتی طیارہ آناًفاناً ٹوٹ کربکھرگیا۔طیارہ کے تمام مسافر،امریکی سفیر مسٹر آرنلڈ رافل،پاکستان کے فوج کے سربراہ جناب جنرل محمد ضیاء،پاکستان کے دولیفٹنٹ جنرل،تین میجر جنرل،پانچ بریگیڈ،ایک کرنل،ایک اسکواڈرن سمیت ۱۳۷ افراد لقمۂ اجل بن گئے۔
حادثہ کی وجوہات کیاہیں؟اعلیٰ پیمانے پرتحقیقات جاری ہیں اوراس سلسلے میں امریکہ نے پاکستان کے ساتھ ہرممکن تعاون اور سی۔آئی۔اے کے ذریعہ تحقیقات میں مدد دینے کی پیش کش کی ہے۔
اس بھیانک حادثہ اوربین الاقوامی سازش کے جو بھی ذمہ دار ہوں حقیقت یہ ہے کہ صدر ضیاء الحق اب اس دنیا میں نہیں رہے۔
صدر ضیاء الحق مرحوم۱۲/اگست۱۹۲۳ء میں پنجاب کے مشہور شہر جالندھر میں ایک متوسط گھرانے میں پیداہوئے۔دہرہ دون کے دون اسکول میں ابتدائی تعلیم کے بعد سینٹ اسٹیفن کالج دہلی میں داخلہ لیا جہاں قبلہ جناب پروفیسر مولانا سعید احمد اکبر آبادی مرحوم سابق مدیر ’’برہان‘‘سے بھی مرحوم صدر کواستفادہ کا موقع ملا اوراس میں کوئی شک نہیں کہ جنرل ضیاء، حضرت اکبرآبادی سے تمام زندگی بے حد متاثر رہے۔۱۹۴۶ء میں دہرہ دون کی رائل ملٹری اکیڈمی سے فراغت کے بعد فوج میں شامل ہوگئے۔۱۹۷۶ء تک وہ ایک جونئیر افسر تھے جنرل ٹکاخاں کے ریٹائر ہونے کے بعد مسٹر بھٹو نے ان کوجنرل بنادیا۔۱۹۷۷ء میں جنرل ضیاء بھٹو کاتختہ الٹ کر خوداقتدار پر قابض ہوگئے۔۱۹۷۷ء میں اقتدار سنبھالنے کے بعد چیف ایڈمنسٹریٹر کی حیثیت سے کام کرتے رہے۔ستمبر۱۹۷۸ء میں مرحوم باضابطہ پاکستان کے صدربن گئے۔۱۹۸۴ء میں مرحوم نے ریفرنڈم کرایا جس کا نتیجہ ان کے حق میں نکلا، اس کے بعد انھوں نے پھر ملک کے...

معاشرتی امن و امان میں پختون روایتی مصالحت اور تحکیم کا کردار: ایک تحقیقی مطالعہ

To maintain and enhance social peace and mutual interaction among people it is mandatory to resolve their mutual conflicts. The eradication of mutual conflicts and working for reconciliation is obligatory on the Muslims. Al-mighty Allah has declared reconciliation and resolution of conflicts among all the Muslims as legitimate action. There are numerous verses of the Holy Quran and Hadith of the Prophet where Muslims have been ordered for reconciliation aiming to promote brotherhood and peace in society. The main rationale behind this is to bring harmony and peace in the social order of life. What are the pre-requisites of reconciliation from Sharia’s perspective, in which conflict reconciliation is permissible and in which cases it is not allowed. This study emphasizes to answer the above mentioned question. Furthermore, efforts have been made to provide a sharia’s foundation for those who are involved in the process of reconciliation in the form of Taḥkīm. This will not only encourage them, but will help in the maintenance of peace in the society. Similarly, a comparison will also be made between the merits and demerits of Pakhtūn’s traditional reconciliation process, and important suggestions will be made to make the Pakhtūn’s traditional reconciliation process more productive and valuable.

Evaluation of Pilot Scale Trickling Filter System for the Treatment of Wastewater

Attached growth wastewater treatment systems are considered to be effective in developing countries due to their low energy, operational and maintenance cost. A trickling filter system is a type of sewage treatment facility which is used to minimize the levels of carbonaceous compounds in terms of BOD5 and COD from wastewater in addition to pathogens and NH4-N level. The present research study was mainly focused on to treat domestic wastewater by pilot scale trickling filter systems. For this tenacity, two different types of pilot scale trickling filter (TF) systems i.e. one was installed in work station (under shade) located at the vicinity of the Department of Microbiology, QAU, Islamabad, Pakistan (setup-I) and the other one was installed in open environment at residential area of QAU, Islamabad, Pakistan (setupII), were evaluated with varied temperature conditions, hydraulic load rates (HLR), organic load rates (OLR) and continuous recirculation of effluent over the top of filter bed. In the 1st phase, efficiency of setup-I system was assessed for potential water reuse, and development of a zero order kinetic model defining the efficiency of carbonaceous and microbial pollutant removal under various prevailing temperature conditions. Operational data (both influent and effluent) of 330 days were collected from pilot scale TF system for various parameters. In rainy monsoon, autumn, spring and summer seasons, average percentage reduction were observed in the range of 52-72, 51-73, 18-69 and 74-89 % for BOD5, COD, TDS and TSS respectively. However in winter season, a high percentage reduction was observed only for TSS (74-81%) while for other parameters like BOD5, COD and TDS, it was found in the range of 13-50, 12-49 and 23-61% respectively. It was also observed that pilot scale treatment facility showed high efficiency in the removal of fecal coliforms i.e. 43-55 and 57-86% average percentage reduction were observed after 1st and 6th day of treatment respectively in all seasons of the year, indicating potential for reuse in irrigation. Moreover, 13 different bacterial species (E. coli, Salmonella, Pseudomonas, Enterobacter, Klebsiella, Shigella, Proteus, Alcaligenes, Staphylococcus, Streptococcus, Micrococcus, Corynebacterium and Bacillus spp.) were isolated from the microbial slime layer developed on the surface of stone media. The mathematical model developed based on data can be used to design and optimize low-cost TF systems aimed at water quality improvement for potential wastewater reuse in developing countries. In the 2nd phase, a cost-effective and simple stone media pilot-scale trickling biofilter (TBF) was designed, constructed and operated in a continuous recirculation mode for wastewater treatment with a hydraulic flow rate of 1.2 L/min (Q = 0.072 m3/hr) and hydraulic loading (Q/A) of 0.147 m3/day for 15 weeks at a temperature range of 14.5-36°C. A substantial reduction in the average concentrations of different pollution indicators, such as COD (85.6%), BOD5 (85.6%), TDS (62.8%), TSS (99.9%), EC (15.1%), PO4 (63.22%), SO4 (28.5%) and TN (34.4%), was observed during 15 weeks of operational period. Whereas a considerable average increase in the levels of DO (63.2%) was found after treatment of wastewater by the TBF system. No significant reduction in most probable number (MPN) index of fecal coliforms was observed in the effluent in first 9 weeks of operation. However, a significant reduction in the MPN of fecal coliforms was observed, i.e. 80-90% in the last few weeks of treatment. In the 3rd phase, stone media setup-II TBF system was operated under different hydraulic retention time (HRT) i.e. 24, 48 and 72 hrs at a constant flow rate of 0.04 m3/day and it was perceived that stone media pilot scale TBF system showed high efficacy regarding removal of physicochemical and microbiological parameters under different HRTs. It was observed that by increasing HRT up to 48 and 72 hrs, the proficiency of setup-II TBF system increased significantly to reduce different parameters i.e. COD (70.9%), TDS (34%), EC (23.5%), SO4 (37%), PO4 (81.8%) and TN (66.6%). Furthermore, it was suggested that greater retention time and sand filtration play a key role in the pathogens removal and improvement of water quality. In the 4th phase, a new strategy was evaluated by using integrated media containing pebbles and gravels instead of stone to provide support for the growth of microbial slime layer. Furthermore, the integrated media TBF system was operated at three different flow rates (Q) i.e. 0.004 m3/day, 0.0072 m3/day and 0.01 m3/day and on each particular flow rate, effluent was operated under three different HRTs i.e. 48, 72 and 96 hrs in order to determine the efficiency of integrated media pilot scale TBF system treating domestic wastewater. It was observed that integrated bed material with air space had optimistic effect over TBF operation and the lowest and intermediate flow rates (0.004 m3/day and 0.0072 m3/day) showed promising results with respect to percent reduction of different physicochemical parameters i.e. COD (74.2-80.5%), TDS (60.3-69.5%), EC (62.8-68.6%) and PO4 (45.3-60.3%). A significant reduction in TN (59-63.3%) was observed at flow rates of 0.004 m3/day and 0.0072 m3/day. Moreover, it was observed that the efficiency of integrated media pilot scale TBF system in terms of pathogen removal (CFU/mL) increased significantly with continuous recirculation of wastewater for an extended period of time under different flow rates. From comparative assessment of stone media and integrated media pilot scale setup-II TBF system, it was evaluated that integrated media TBF system showed significant activity in the percent reduction of different physicochemical parameters as compared to stone media TBF system. Thus, overall results suggest that pilot-scale TBF has a great potential to be transferred to decentralized treatment system for handling sewage of small communities in developing countries, in order to produce effluent of good quality, which can be safely used for irrigation as well as ornamental purposes.