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Home > Ethnoecological Studies of Herbaceous Flora of Sahiwal Division, Punjab Pakistan

Ethnoecological Studies of Herbaceous Flora of Sahiwal Division, Punjab Pakistan

Thesis Info

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Author

Ahmad Ali

Program

PhD

Institute

Government College University

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Botany

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/13972/1/Ahmad_Ali_HSR_2017_Botany_GCU_Lahore_15.02.2018.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726098600

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The present work deals with the ethnoecological studies on the herbaceous flora of Sahiwal Division with special reference to the documentation of ethnobotanical data, phytosociological notes and ethnopharmacological testing of ethnomedicinally important herbs including their antimicrobial, antioxidant and anthelmintic potential. The local people, herbal healer/ hakims were interviewed through questionnaire to collect and document the ethnobotanical data on the local herbaceous plants. In total, 180 herbal plant species, 177 belonging to 43 angiospermic, one species to one gymnospermic and two to pteridophytic families, were found in common use by the local inhabitants as forage / fodder, medicinal, food, feed and various other uses. The crude extracts of five ethnomedicinally important plants were obtained in polar and non-polar solvents (Water, methanol, chloroform and nhexane) by maceration method. The % age yield was found maximum in Cuscuta campestris distilled water extract and minimum in Aerva javanica n-hexane extract. The antimicrobial activity tested by agar well diffusion method indicated strongest antibacterial and antifungal activity by Persicaria glabra against Psedumonas aeruginosa and Aspergillus niger, respectively. The antioxidant activity was found quite convincing when revealed through six different assays. The highest antioxidant activity was shown by Cistanche tubulosa as revealed by ABTS+ Assay, TPC determination, TFC determination and Total antioxidant activity. The highest anthelmintic activity was shown by Cistanche tubulosa while least by Polygonum glabra. Vegetation of the study area was sufficient only for documentation, but insufficient for any economic purpose, yet some plants had shown very important phytosociological attributes. There was no much change in the soil parameters, yet ecological parameters like frequency, distribution and community structure were changing district wise. The TWINSPAN analysis of the vegetation of the study area divided it in to 3 groups and 12 associations, consisting of 159 plant species, in 222 quadrats.
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بھانویں خواب اندر اوہ آگئے

بھانویں خواب اندر اوہ آگئے
ہک وار تاں من پرچا گئے
پا سرمہ نین نشیلیاں وچ
نیناں دے تیر چلا گئے
توں لبھناں ایں پھل بہاراں دے
ساڈے اگلے وی کُرما گئے
اساں رو رو حال سناندے رہے
اوہ چپ دے جندرے لا گئے
ساڈے کولوں آ کے ٹر گئے
تارے چٹے دینہہ وکھا گئے
سُن ناں کرونا وائرس دا
لوکی حجریاں وچ سما گئے

5 The Gambia's NYSS Partnership with the International Organization for Migration (IOM)

The paper examines the dynamics of The Gambian National Youth Service Scheme (NYSS) founded in 1996 through the efforts of Nigeria’s National Youth Service Corps (NYSC) Scheme. Though modeled after the NYSC, it has ventured into things NYSC has not. One of these was its most recent partnership with the IOM to curb recurrent illicit migration of The Gambian youths. The questions are, has this aspect of Nigeria-The Gambia history been documented? Has Nigerian Scheme gone into partnership with the IOM in the enterprise like The Gambian counterpart? Since its establishment, how many times has NYSC adjusted to changing realities? In what theoretical contexts could one frame the argument of the NYSS/ IOM partnership and what lessons does that holds for NYSC and similar youths’ services in Africa in the century? In addressing the problem, the paper adopts the qualitative method of historical research and gleans on evidence from diverse sources. It foregrounds its argument on the ideas of the National Youth Service as “Moral Equivalent of War”, “Service- Learning” and “A Way of Strengthening Ties among the People of the World” espoused by William James, John Dewey, and Rosenstock-Huessy. It concludes by charging NYSC and similar services to adjust to meet the current challenges of African youth and society

Molecular Genetics of Autosomal Recessive Retinitis Pigmentosa in Consanguineous Pakistani Families

It is an established fact that genetic disorders are one of the most important threats to human health. Several genetic disorders have been described clinically but their etiology is still unidentified and mysterious. The molecular basis for most of them is also unknown. With the advancement in the field of molecular biology different powerful techniques have been developed to understand the molecular basis of hereditary disorders. This would help in the subsequent identification of causative genes and mutations. Blindness and visual impairment due to genetic disorders are more common in developing countries like Pakistan than in developed countries. Retinitis pigmentosa (RP) is a major form of incurable blindness affecting one out of 4000 people worldwide. This highly heterogeneous disease has numerous inheritance patterns with the end result of partial to complete irreversible blindness. Another ocular disorder called fundus albipunctatus (FAP) also has some symptoms similar to RP like night blindness. In FAP this night blindness occurs in childhood but it remains stationary and day vision is not affected as in the case of RP where constriction of day vision occurs gradually. The present study was aimed to analyze families with ocular disorder. Families with autosomal recessive hereditary retinitis pigmentosa were used for mapping the disease genes and mutations. Seven consanguineous unrelated families (RP8, RP9, RP11, RP12, RP13, RP14 and RP16) with inherited RP were ascertained from different regions of Pakistan. The mode of inheritance in all families was inferred as autosomal recessive. The strategy used for this study was candidate gene approach. Linkage analysis was performed by PCR using STR (short tandem repeats) microsatellite markers for the known loci/genes. Direct sequencing (next generation sequencing) of the PCR products was carried out for identification of pathogenic mutations. In the present study linkage to crumbs homolog 1 (CRB1) gene on chromosome 1q31.3 was confirmed in family RP12. A novel missense mutation in human CRB1 gene has been found after sequence analysis of exon 6 of the CRB1 gene at nucleotide position xx 1459 (c.1459T>C). At protein level this mutation resulted in a substitution of proline for serine at amino acid 487 (p.Ser487Pro). It was inferred that mutation in this gene is strong enough to cause autosomal recessive retinitis pigmentosa. After the initial screening of autosomal recessive retinitis pigmentosa loci for family RP13, it was evident that there was no involvement of retinitis pigmentosal loci in the disease phenotype and it was a rare case of fundus albipunctatus, with RDH5 gene defect as the underlying cause. The family RP13 showed linkage to retinol dehydrogenase 5 (11-cis/9-cis) RDH5 gene after homozygosity mapping. A novel missense mutation at nucleotide position 602 (c.602 C>T) was identified after next generation sequencing of exon 4 of the RDH5 gene .This mutation resulted in substitution of phenylealanine for serine at amino acid 201 (p.Ser201Phe) of the RDH5 gene. The mutations in RDH5 gene are related to fundus albipunctatus (FAP). This is an exceptional form of stationary night blindness, it was deduced that mutation in this gene was responsible for autosomal recessive FAP in this family. The family RP14 showed exclusion to all the known genes and loci of RP. It was inferred that a novel locus/gene is responsible for causing RP in this family. The strongest candidate gene was RY2R which was earlier involved in cardiac disorder. Fine mapping in future would confirm the involvement of this gene in RP. Four families (RP8, RP9, RP11 and RP16) with some of the common selected loci/gene showed heterozygosity for the different combinations of the parental alleles in both affected and normal individuals after the linitial linkage. This heterozygosity confirmed exclusion to five selected known loci or genes on different chromosomes associated with autosomal recessive RP. Since many genes and loci are involved in this disease and genotyping using vertical polyacrylamide gel electrophoresis (PAGE) is a time taking and laborious method so commonly found genes in RP were initially selected which showed exclusion.On the basis of these exclusions it was inferred that a novel locus/gene or mutation is involved in these families which could be identified by SNP affymetrix array technique and sequencing. Many loci/genes/mutations are yet to be identified for this phenotype. It would be helpful in future to understand the disease prognosis. This research will also provide a smooth way for carrier screening, genetic counseling and prenatal diagnosis. This study may help gaining insight into the genetic causes underlying these disorders, to improve the clinical management and prevention.