Acer pentapomicum (Family Aceraceae) is commonly known as maple plant. The aim of the study was to evaluate A. pentapomicum for biogenic synthesis of nanoparticles, its characterization, pharmacological importance and micro propagation. A. pentapomicum leaves mediated gold and silver nano-particles were successfully synthesized by reacting 1ml of aqueous leaves extract with different ratios of 1mM AuCl3 and AgNo3 solutions. The synthesis of gold and silver nanoparticles were confirmed and characterized by visual observation, UV-Visible-spectroscopy, scanning electron microscopy (SEM), Energy Dispersive X-ray (EDX), X-Ray Diffraction (XRD) and Fourier Transform Infrared FTIR analysis. Color change from yellowish to dark purple and to reddish brown confirmed the gold and silver nanoparticles synthesis. Gold nanoparticles (AuNps) were stable at 25-35°C, at pH 7-8 and in the presence of millimolar salt (NaCl) solution. Biosynthesized nanoparticles display a characteristic surface plasmon resonance at 568nm (AuNps) and 450nm (AgNps) in UV-Visible spectrum. SEM and EDX analysis also confirmed the presence and synthesis of gold and silver nanoparticles with spherical morphology. XRD patterns reveals the cubic, face centered, crystalline nature of gold and silver nanoparticles. The leaves mediated silver nanoparticles, however, were efficiently produced at a temperature range of 35-55°C and an optimum pH of 6-7. The average size of the synthesized gold and silver nanoparticles found to be in the range of 18-25nm. The FTIR analysis of gold and silver nanoparticles affirmed the presence of certain biomolecules (carboxylic acid, flavonoids, phenols) that act as reducing and capping agent for leaves mediated nanoparticles synthesis. Agar well diffusion method was carried out for potent biocidal activities of gold and silver nanoparticles against different bacterial, fungal and candida strains. Both synthesized nanoparticles showed potent bactericidal, fungicidal and candidacidal activities. However, Klebseilla pneumonia with growth inhibition of 91% was the most susceptible microbe to gold nanoparticles. Antimicrobial activities of A. pentapomicum (leaves and stem) extracted with different organic solvents were also evaluated using Agar disc diffusion and well diffusion bioassay. Leaves extracted samples showed better biocidal activities than stem extracted samples. The most susceptible bacterium was Pseudomonas aeruginosa (79-94%) and Escherichia coli (87-91%), while, the least susceptible was Staphylococcus aureus (26-50%). Citrobacter freundi was the most resistant microbe to different plant extracts. Aspergillus niger was the most inhibited fungal species by both stems and leaves extracts followed by Rizopus oryzae. Regarding the antioxidant activity, different plant extracts and its synthesized nanoparticles possessed proficient antioxidant activity. However, methanolic extract of leaves and ethyl acetate extract of stem recorded maximum antioxidant activity. Gold nanoparticles showed better antioxidant activity than silver nanoparticles. When evaluated for phytotoxic activity, all plant extracts proved to be toxic against Lemna minor, revealing Butanol extract with maximum toxicity (87%). The qualitative analysis of different solvent extracted fractions confirmed the presence of various phytoconstituents such as alkaloids, terpenoids, flavonoids, tannins, saponins, fixed oil, carbohydrates, and proteins etc. A successful micro propagation protocol for the shooting was developed and it was found that full strength woody plant medium (WPM) supplemented with BAP + IAA (2+0.5 mg l-1), recorded maximum shoot induction. Based on these results the plant can be efficiently used for safe, ecofriendly nanoparticles production, and as a biocidal, an antioxidant and a herbicidal agent.