A study was planned to assess the salt tolerance potential of 10 wheat cultivars, five newly developed (S-24, Saher-2006, Inqlab-91, Faisalabad-2008 and Lasani) and five candidate (P.B-18, M.P-65, SH-20, AARI-10, G.A-20). Two salinity levels were (0 mM and 150 mM) applied at three growth stages seedling, tillering and boot stages. This study comprised independent two experiments. The first experiment was performed to screen the 10 wheat cultivars on the basis of biomass production and yield under saline stress. Four wheat cultivars (S-24, Saher-2006, Lasani and AARI-10) were selected on the basis of the results of the first experiment and again grown in the following year for detailed study of physio-biochemical responses under saline stress imposed at different growth stages. Saline stress application at different growth phases caused a marked reduction in biomass production, yield, different gas exchange attributes, photosynthetic pigments and water relation parameters. However, comparatively cvs. S-24 and Saher-2006 showed less reduction in these attributes due to saline application at three growth stages. Less cholophyll degradation, comparatively higher photosynthetic and transpiration rate and leaf turgor were observed due to saline stress imposition at the boot stage. However, it was greater due to salinity imposed at the seedling stage. Furthermore, NaCl applied at three different growth stages markedly increased the endogenous Na+ level and caused reduction in Ca2+, K+, K+/ Na+ and Ca2+/ Na+ ratios. Overall, cvs. Saher-2006 and S-24 maintained higher K+/ Na+ and Ca2+/ Na+ ratios as compared to the other wheat cultivars under salt stress. There was a less decrease in K+/ Na+ and Ca2+/ Na+ ratios under the saline environment developed at the boot stage while maximum at the seedling stage. A significant alteration in different biochemical attributes was observed in all wheat cultivars under saline stress imposed at three growth stages. For example, a substantial increase was observed in H2O2, MDA, soluble proteins, proline, glycinebetaine and activities of different aintioxidants (SOD, POD, CAT, phenolics). Different wheat cultivars showed variation in these biochemicals in response to salt stress applied at different growth stages. Maximum antioxidative enzymes activities were observed in cvs. S-24 and Saher-2006 due to saline stress application at three growth stages. However, cvs, S-24 and Sher-2006 had higher accumulation of GB and proline as compared to the other cultivars (Lasani and AARI-10) under saline stress imposed at early growth stages (seedling and tillering) than at the boot stage. Anatomical studies of four wheat cultivars under saline stress applied at different growth stages showed that cvs. S-24 and Saher- 2006 had thick epidermis of stem and root, greater schlerification in stem and leaf, higher vascular bundle and metaxylem area and greater cortical cell area due to saline stress applied at three different growth stages than the other two cultivars (Lasani and AARI- 10). These anatomical characteristics showed their better adaptability under saline environment in terms of checking water loss, good water storage capacity, and better toxic ion compartmentalization due to large size vacuoles of cortical cells and better water and nutrient transport through vessels. So, it can be inferred that wheat is more prone to adverse effects of saline stress when applied at the early growth stage (seedling) than at later growth stages. Overall, of all wheat cultivars cvs. S-24 and Saher-2006 were more tolerant to saline stress.
3۔ قتل خطاء قتل خطاء کی مندرجہ ذیل دو صورتیں ہیں : 1۔ خطاء فی القصد فاعل (قاتل )کے قصد اور ارادہ میں خطاء اور غلطی واقع ہو جائے ۔ اس کی صورت یہ ہے کہ کسی شخص پر تیر یا فائر چلایا یہ خیال کرتے ہوئے کہ وہ شکار ہے مگر اتفاق سے وہ آدمی نکلا یا یہ کہ کسی شخص کو حربی کافر سمجھتے ہوئے نشانہ بنایا ، لیکن بعد میں معلوم ہو ا کہ وہ مسلمان ہے ۔ 2۔ خطاء فی الفعل فاعل ( قاتل) کے فعل میں خطاء ہو جائے ۔ اس کی صور ت یہ ہے کہ کسی نے نشانہ پر تیر مارا لیکن وہ کسی اور آدمی کے لگ گیا (نشانہ چوک جانے کی وجہ سے)196۔ قتل خطاء کے احکام ﴿ وَمَا كَانَ لِمُؤْمِنٍ أَنْ يَقْتُلَ مُؤْمِنًا إِلَّا خَطَأً وَمَنْ قَتَلَ مُؤْمِنًا خَطَأً فَتَحْرِيرُ رَقَبَةٍ مُؤْمِنَةٍ وَدِيَةٌ مُسَلَّمَةٌ إِلَى أَهْلِهِ إِلَّا أَنْ يَصَّدَّقُوا فَإِنْ كَانَ مِنْ قَوْمٍ عَدُوٍّ لَكُمْ وَهُوَ مُؤْمِنٌ فَتَحْرِيرُ رَقَبَةٍ مُؤْمِنَةٍ وَإِنْ كَانَ مِنْ قَوْمٍ بَيْنَكُمْ وَبَيْنَهُمْ مِيثَاقٌ فَدِيَةٌ مُسَلَّمَةٌ إِلَى أَهْلِهِ وَتَحْرِيرُ رَقَبَةٍ مُؤْمِنَةٍ فَمَنْ لَمْ يَجِدْ فَصِيَامُ شَهْرَيْنِ مُتَتَابِعَيْنِ تَوْبَةً مِنَ اللَّهِ وَكَانَ اللَّهُ عَلِيمًا حَكِيمًا ﴾197 "کسی مومن کی شان نہیں کہ وہ کسی مومن کو قتل کرے لیکن غلطی سے ہو جائے تو اور بات ہے اور جو شخص کسی مومن کو غلطی سے قتل کر دے تو اس پر ایک غلام یا لونڈی کا آزاد کرنا ہے اور خون بہا بھی جو اس کے خاندان والوں کو دیا جائے مگر یہ کہ وہ لوگ معاف کر دیں اور اگر وہ ( مقتول خطا) تمہارے مخالف قوم سے ہے اور وہ خود مومن ہے تو ایک غلام یا لونڈی آزاد کرنا پڑے گا اور اگر وہ ایسی قوم سے ہے کہ تم میں اور ان میں معاہدہ ہے تو خون بہا بھی...
During the former era of the State of Bahawalpur the Nawabs were in perpetual conflict with their relatives. The Kehlwar family of Sindh and during the modern era remained under influence of the British. So we can say that the State of Bahawalpur remained under constant foreign influence and the Nawabs did not have chance to rule with liberty and ease. In spite of these facts, the government of the State had many Islamic qualities and there are clear effects of Fatwa on judicial system in both eras.
Pioglitazone is an oral anti-diabetic agent which belongs to a class “thiazolidinediones”. It is used in the treatment of type 2 diabetes mellitus. It acts primarily by increasing peripheral sensitivity to insulin through binding to peroxisome proliferators activated receptor gamma. In recent years, due to variation in pharmacokinetic parameters under different environmental conditions, drug pharmacokinetics has received increasing attention. Therefore, the present study was designed to evaluate the biokinetics, renal clearance and effect on glycation level of pioglitazone in human beings under indigenous conditions. After through clinical examination healthy male volunteers (n = 24), female volunteers (n = 12) and diabetic patients (n = 8) were selected for the study. Each volunteer was given a therapeutic dose of 30 mg pioglitazone tablet orally. Patients were given a dose of 30 mg pioglitazone daily up to 12 weeks. In healthy volunteers scheduled plasma and urine samples were collected at different time intervals. Concentration of pioglitazone in plasma and urine samples was determined by validated high performance liquid chromatographic method. For the estimation of renal clearance of pioglitazone, the endogenous creatinine level was measured in plasma and urine samples spectrophotometrically using kit method based on Jaffe reaction. In plasma samples obtained from healthy volunteers and patients, amount of glucose was estimated by kit method, proteins by Biuret method and glycation level was measured by Thiobarbituric acid (TBA) method, spectrophotometerically. Both differences and similarities are present in the values of different calculated parameters and the values reported in the literature. After comparison minor sex differences were also found to be present in the values of different calculated parameters but statistically these differences were found to be non-significant. A single dose of pioglitazone (30 mg) has no effect on glucose concentration and glycation level in healthy male volunteers. Long term treatment of pioglitazone in diabetic patients, significantly decreased the glucose concentration and glycation level. This indicates that pioglitazone acts as an inhibitor of glycation.