Search or add a thesis

Advanced Search (Beta)
Home > Evaluation of Synthetic Epsps Gene in Genetically Transformed Plants

Evaluation of Synthetic Epsps Gene in Genetically Transformed Plants

Thesis Info

Access Option

External Link

Author

Imran, Muhammad

Program

PhD

Institute

Pakistan Institute of Engineering and Applied Sciences

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Biotechnology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12812/1/M.%20Imran%20Thesis%20%28PIEAS%2c%2010-7-1-059-2010.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726142648

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


Codon optimized synthetic epsps gene was used to develop transgenic tobacco and sugarcane lines using Agrobacterium and biolistic approach, respectively. Putative transgenic tobacco lines analyzed by qRT-PCR showed variable mRNA transcript levels. Southern hybridization performed for determining gene integration patterns showed multiple copy inserts (1-4) of transgene in various tested lines. Immunoblot strips confirmed the translation of cp4-epsps mRNA transcripts in T1 line. Malachite green assay showed variable EPSPS activity levels in various lines in response to glyphosate application. Transgenic tobacco lines exposed to 1% (v/v) glyphosate at 4 to 5 leaf stage showed variable resistance levels ranging from moderate level tolerance to high tolerance. The cp4-epsps mRNA transcript levels varied from genotype to genotype in tested transgenic sugarcane lines regardless of the integration of multiple copy number (3-6) of transgene. Genetic transformation with pyramided constructs results in the integration of multiple genes at a single locus, which is desirable in terms of expression efficiency of transgenes. The cp4-epsps gene was pyramided with insect resistant cry1Ac and cry2Ab genes and cloned in pSb187 vector was used to genetically transform tobacco using Agrobacterium mediated approach. Transgenic lines were confirmed through PCR using gene specific primers of respective genes. The mRNA transcript level of cp4-epsps gene detected through qRT-PCR using GAPDH gene as an internal control was found to be variable among different transgenic lines. Transgenic lines were tested for checking the translational efficiency of all three genes using immunoblot strips. Transgenic tobacco lines found expressing all three proteins were subjected to detached leaf insect bioassays to evaluate the effectiveness of Bt genes in putative transgenic tobacco against insect attack using first instar larvae from Spodoptera littura and Helicoverpa armigera. Transgenic tobacco found to be resistant against these insects showed that cry1Ac and cry2Ab genes were efficient enough in pyramided form. When these transgenic tobacco lines were exposed to glyphosate at 10 to 12 leaf stage, chlorotic symptoms were observed which hinted that herbicide tolerance potential of transgene declined with increasing age of the plants. In order to overcome the spatio temporal gene expression issues, a dual copy epsps expression cassette synthesized under different regulatory elements, cloned in pGreen0029 binary vector was used to generate transgenic tobacco. These tobacco lines exhibited excellent resistance against 19% (v/v) glyphosate at 10-12 leaf stage. This construct has shown tremendous potential regarding transgene expression levels which is required in the field crops. This dual cassette cp4-epsps gene has been recommended for generation of glyphosate resistant cotton and other commercially important cultivars.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

زبان کی مختلف سطحیں(معنویات)

موضوع6: زبان کی مختلف سطحیں(معنویات)
معنویات:
وہ علم ہے جو معنی اور اس کے متعلقات سے بحث کرتا ہے اور معنی وہ ذہنی شبیہ ہے جو ہر لفظ کی صوتی شبہ کے پیچھے چھپی ہوتی ہے۔چنانچہ لفظ اور معنی کا رشتہ اٹوٹ ہوتا ہے۔ لفظ سے معنی اور معنی سے لفظ جدا نہیں ہو سکتا۔ جس طرح الفاظ کی آوازوں کا مطالعہ صوتیات کہلاتا ہے اسی طرح معنی کا مطالعہ معنویات کہلاتا ہے اور یہ دونوں لسانیات کے اہم حصے ہیں۔ یعنی لفظ وہ اشارہ ہے جس کی طرف معنی اشارہ کرتا ہے اور زبان دونوں کے ربط کا دوسرا نام ہے۔ پروفیسر جوز شور کہتے ہیں :
‘‘انسانی معاشرے میں لفظ کی جو قدروقیمت ہے وہ صرف اس کے معنی کی بدولت ہے جو اس میں چھپا ہوتا ہے ناکہ ان مفرد آوازوں کی جن سے لفظ مرکب ہوتا ہے"
آوازوں کے بے مقصد مرکب کے لحاظ سے لفظ کو بھی لسانیات میں کوئی منزلت حاصل نہیں ہو سکتی۔جس سے یہ نتیجہ نکالا جاتا ہے کہ فطرت انسانی کے نقطہ نظر سے معنی کو لفظ پر ترجیح حاصل ہے۔ بعض اوقات لوگ زبان کی طرح معنی میں بھی تعریفیت کا سراغ لگاتے ہیں۔ڈاکٹر سہیل بخاری کہتے ہیں:
"اس غلط سوچ نے علم بیان کے محققوں کو بہت بھٹکایا ہے معنویات مطالعہ معنی ہے اور مطالعہ معنی گرامر کا مطالعہ ہے۔"
یہ خیال بھی پچھلے خیال کی طرح بے بنیاد ہیں معنویات مطالعہ معنی ضرور ہے لیکن گرامر کا معنی یا مطالعہ معنی سے کوئی تعلق نہیں رکھتا گرامر کلام کے ظاہر یا ہیت کا مطالعہ کرتی ہے اور اس کے اجزا اور ارکان کے درمیان باہمی روابط کو توجہ کا مرکز بناتی ہے اس طرح گرامر اور معنویات کا دائرہ ایک دوسرے سے الگ ہیں۔
لفظ اور معنی کا تعلق:
زندگی ایک با مقصد حقیقت ہے...

لسانیات اورمعاشرتی روابط

لسانیات اورمعاشرتی روابط Language is fundamental to any society. It is through language that we can communicate our thoughts to others. The more a language is used, the more it will develop. There are many types of languages including mother tongue, regional language, religious language, official language, business language, national language, international language and so on. When speaking, language and ascent are not taken into consideration, but there is a need to be careful while writing. People who cannot speak a language use specific gestures or symbols to convey their point of view to others. Therefore, we can say that the use of language began as soon as man came into this world.

Hybrid Reasoning Approach in Clinical Decision Support System

Through the advent of technological progression, different computer aided applications were introduced during last four decades to supplement the diagnosis and treatment phases of patient care. Now at different levels initiatives have been taken to encourage the medical practitioners for implementing these high-tech computer applications in their everyday clinical practices to enhance the graph of human well-being. Clinical decision support systems (CDSSs) were introduced as an ideal computer based application to influence the medical diagnosis process with its capability to store large extent of data and provide prerequisite data at the time of patient evaluation phases without wasting time. However the efficient progression of CDSS impeded by a number of obstacles which if addressed could potentially unlock the significance of these systems. This research work reveals the comprehensive detail of the different CDSSs that were proposed during the last four decades after the innovation of these computer systems and then draw attention towards the desirable features of CDSSs that were found as the research gaps during literature review. This study is conducted with an aim to provide a CDSSwhichisproficientenoughtoovercomethegrandchallengesthatwerearoseduring the effective deployment of these systems. ThisresearchworkpresentsanonlineKnowledgeBasedClinicalDecisionSupportSystem (KBCDSS) that is deployed as an effective prototype application in medical domain to significantly aid medical experts in their routine clinical practices. KBCDSS is a multiple disease diagnosis system with the proficiency to gather medical experts over a single platform through web. This system follows the pattern of Knowledge Data Discovery (KDD) process to extract the knowledge that is prerequisite in the patient evaluation stages. In order to accomplish an effective functioning of this system certain course of action is followed for data analysis on the Wisconsin breast cancer data set from the UCI Machine Learning Repository and implements that medical data set on the proposed system. The proposed KBCDSS initially pursues the pre-processing steps of KDD process to perform the knowledge acquisition task and proposed knowledge acquisition algorithm which efficiently update prerequisite medical data into data warehouse. The data warehouse server retains different medical records that are stored in relational tables. Using thetechniqueofDBMSwehaveproposedanalgorithmfortheconstructionofKnowledge Base (KB) and its representation. Toperformminingtask,wehaveproposedhybridCaseBaseReasoning(CBR)cyclewhere CBRandSupportVectorMachine(SVM)areusedasaninferencemechanismtocarryout more accurate conclusion and results. CBR is implemented as a core methodology in our model and we have proposed case retrieval algorithm for case retrieval phase of the CBR technique that are retrieving similar cases from KB. After that reinstantiation strategy is implemented in case reuse phase of the CBR technique for case adaption, that is simply copy the diagnosis of most similar case being the suggested solution of new input case. In case, alike cases are not present in KB, then we employ SVM for predicting the solution for new case. SVM is used for classification of data as well as predict the solution of new input case. After that, the concept of Group Clinical Decision Making (GCDM) is implemented in case revise phase where number of experts of same medical domain gives their opinion for the solution of new input case. For positive opinion from medical experts,newcaseisnowkeptintoKBwhichisthepartofrelationalDBforfutureguidance. The proposed KBCDSS is competent enough to provide comprehensive structural knowledge to its users within the very short span of time which is extremely supportive during the process of diagnosis and treatment of diseases. The efforts to develop this application were aimed to fulfill the research gaps and strengthen the weakness of previously existing CDSSssothatthedeploymentofthesecomputerbasedsystemsbecomegeneralandevery medical personals can also easily use these systems by their own without the supervision of computer experts during the patient-care phases.