Search or add a thesis

Advanced Search (Beta)
Home > Exploration of Anthelmintic, Antidiabetic and Anticancer Potential of in Vitro Cultures of Cichorium Intybus L. , Peganum Harmala L. and Withania Somnifera L. Dun.

Exploration of Anthelmintic, Antidiabetic and Anticancer Potential of in Vitro Cultures of Cichorium Intybus L. , Peganum Harmala L. and Withania Somnifera L. Dun.

Thesis Info

Access Option

External Link

Author

Begum, Sajida

Program

PhD

Institute

Government College University

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Botany

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/10223/1/Sajida%20Begum_Botany_2019_GCU%28L%29_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726159858

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


The present study deals with the phytochemical analyses and determination of antimicrobial, anthelmintic, antidiabetic and anticancer potential of in vitro plant tissue and callus culture extracts of Cichorium intybus L., Peganum harmala L. and Withania. somnifera (L.) Dun. Maximum seed germination was observed in P. harmala seeds. Explants such as hypocotyl and cotyledon from in vitro seedlings were cultured on MS medium supplemented with different combinations and concentrations of plant growth regulators for callus induction. The most successful culture media were 0.05 TDZ + 0.5 NAA mg/L, 0.05 TDZ + 0.5 IBA mg/L and 0.05 TDZ + 0.5 BAP mg/L for callus induction of C. intybus, P. harmala and W. somnifera two for each respectively. Four different solvents depending upon polarity were used for extraction of explant tissues and callus cultures. Maximum percentage yield was recorded in chloroform extract of cotyledon callus cultures of C. intybus followed by chloroform extract of hypocotyl callus of P. harmala and cotyledon callus of W. somnifera respectively. Phytochemical analyses of extracts indicated the presence of valuable phytochemicals such as alkaloids, flavonoids, phenols, saponins, tannins, reducing sugars and terpenoids. Antimicrobial potential was tested against pathogenic bacteria (Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa) and fungi (Aspergillus niger and Fusarium solani). Maximum inhibition zones were recorded in methanol extracts of P. harmala and C. intybus hypocotyl callus culture against B. subtilis and E. coli respectively followed by chloroform and methanol extracts. Anthelmintic test showed that methanol extract of cotyledon callus culture of P. harmala exhibited considerable anthelmintic potential. Antidiabetic potential of methanol extracts of callus cultures was evaluated by using rabbits as experimental organisms. The extract of C. intybus hypocotyl callus culture showed maximum decrease in blood sugar level followed by the W. somnifera cotyledon callus culture in streptozotocin induced diabetic rabbits. Anticancer activity was conducted through HTC116 human colon cancer cell lines cultured in Dulbecco’s Modified Eagle Medium. Methanol extract of P. harmala hypocotyl callus cultures showed highest anticancer potential followed by chloroform extract of hypocotyl callus culture of C. intybus. It can be concluded that callus cultures of the plants selected for study hold auspicious ethno-pharmacological effectiveness and in vitro biomass production may be helpful for biodiversity conservation
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

راجیو گاندھی

راجیو گاندھی
وسط مدتی انتخابات کے دوران میں مدراس کے ایک انتخابی جلسہ میں تقریر کے پروگرام سے کچھ دیر پہلے ہی ہندوستان کے سابق وزیراعظم اور انڈین نیشنل کانگریس کے صدر جناب راجیوگاندھی ایک بم دھماکے میں لُقمۂ اجل ہوگئے۔
اس وسط مدتی انتخاب کے بارے میں جہاندیدہ دانشورانِ ملک اور خودمقتول ...راجیوگاندھی یہ خدشہ ورائے ظاہر کررہے تھے کہ اس وسط مدتی انتخاب میں زبردست تشدّد کاامکان نظرآرہا ہے ۔یہ کس کومعلوم تھاکہ جس تشدّدکے امکان کااظہار کیاجارہاہے وہ ملک کی اس عظیم ہستی ہی کو اپنی منحوس لپیٹ میں لے لے گا۔مگرانہونی ہوکررہی اورملک اایک ایسے رہنما سے محروم ہوگیا جو دورِ حاضر کاہیرو تھا اور مستقبل کی روشن قندیل، اور جس سے ملک و کوبڑی بڑی امیدیں وابستہ تھیں۔
جناب راجیو گاندھی کے حادثۂ قتل میں کس پارٹی، کس گروہ ،کس ملک یا کس فرد کاہاتھ ہے اس کے بارے میں کچھ کہنا قبل از وقت ہے۔ابھی تک کسی نے اس کی ذمہ داری قبول نہیں کی ہے۔عام طور پرایل ۔ٹی۔ٹی دہشت پسندوں پرشک ظاہر کیا جارہا ہے۔اوراس کے لیے اخبارات کی اطلاع کے مطابق کچھ ٹھوس ثبوت بھی جائے واردات سے مِلے ہیں۔ جو عورت اپنے جسم پر بم باندھ و لپیٹ کر خودبھی ہلاک ہوئی ہے اور جناب راجیوگاندھی کے ساتھ اوردوسرے تیرہ افراد کوہلاک کرنے کاباعث بنی ہے اس کے متعلق بھی عام قیاس یہ ہی ہے کہ وہ ایل ٹی ٹی گروہ سے تعلق رکھتی ہے۔مگرابھی یہ سب قیاس آرائیاں ہی ہیں،یقین و قطعیت سے کچھ نہیں کہا جاسکتا ہے۔حکومتِ ہند نے تمام حادثۂ قتل کی تحقیقات کے لیے بروقت ایک کمیشن بنادیا ہے جوعرصہ تین ماہ میں اس سلسلے میں اپنی رپورٹ پیش کرے گا۔ اس سے پہلے جوبھی اس سلسلے میں اظہار ِ خیال کرے گا وہ صرف قیاسات ہی کے زمرے میں ہوگا۔اورحقیقت ویقین کاگمان کرنا...

When Comes such Another? Asma Jahangir 1952 –2018

On the 11th of February this year death vanquished Pakistan’s Asma Jahangir: Nothing else could. Her name will endure; yet one cannot go on to say “death thou art dead” for the vacuum in the field where this indomitable and intellectually gifted lawyer fought and won her battles for the forgotten and ignored, the resource-less, and – above all – for the politically and socially persecuted is felt more gravely with each passing day: Asma Jahangir was a convinced human rights activist. There are many such, but she was a uniquely effective and successful one.

Next Generation Energy Efficient Process Protocols in Si-Cmos Electronics

As Silicon CMOS manufacturing industry is possibly reaching to its climax with complex requirements posed by the International Technology Roadmap of Semiconductors; a “More Moore” approach is fully utilized to further exploit the Silicon Fabrication protocols. This goes hand in hand with other two process and design strategies, namely, “More than Moore” and “Beyond CMOS”. A 10 node technology, converging the chip fabrication processes between 10nm and 20nm, has been rigorously tested and revisited to look for complimentary solutions which are exploitable in variety of designs and processes in next generation technology nodes. The operational output characteristics of MOS/CMOS and its variant devices are being reassessed with a perspective of energy efficiency gained during the device design (inclusive of scaling requirements) and process development. This, in turn, influences the power consumption, proficiency of performance and reliability of the devices. Such energy efficient electronics has gained a huge interest due the evolution of Internet of Things (IoT) and its ultra-low power considerations. This study focuses on the energy efficient design and fabrication of Silicon CMOS/MOSCAP devices with a detailed electrical and electro-optical metrology. A FAB-specific MOSFET design (technology file), simulated output characteristics, half-of-FAB line processing of MOSCAP structure with integration of novel Atomic Layer Deposited TiN/HfSiOx metal gate/High-k stack and atomistic-layered PVD-driven Aluminium back-contact metallization is realized in this work. Energy efficiency is also studied through the concept of thermal budget by virtue of the dynamics of annealing which greatly influence the electrical properties of the gate stack and consequently the reliability of the process. Subsequent process characterization is performed using Current-Voltage, Capacitance-Voltage, Current Density, Leakage Resistance, Built-in Voltage, Doping Profiling, Charge-storage/ Permittivity, Hall Effect and Vertical Field analysis. Sophisticated metrology techniques such as Charge-Deep Level Transient Spectroscopy and Spectroscopic Ellipsometry are utilized to extract and evaluate the hypersensitive electrical and electro-optical parameters such as charge-transient behavior, activation energy, trap density, capture cross-section, refractive index, dielectric and extinction coefficients etc. Design verification through FAB-line processing, post-FAB thermal processing and variety of measurements revealed a number of interesting results which altogether provide a novel process window to engineer energy efficient electronics with a better trade-off. These results may have ramifications for device and process engineer.