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Home > Extraction & Exploration of Zein Protein As Potential Encapsulating Material

Extraction & Exploration of Zein Protein As Potential Encapsulating Material

Thesis Info

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Author

Siddique, Tahira

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Food Science & Technology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12759/1/Tahira_Siddique_Food_Technology_HSR_2018_UAF_14.09.2018.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726175914

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Maize is third important cereal crop in Pakistan and is used for both feed and fodder purpose. It is a good source of protein which is not only important in the human body but also widely used in the industry. Zein is a valuable maize protein that is hydrophobic in nature. It has potential use in different industries. The objective of research was to extract and explore potential of zein as an antimicrobial agent, moisture barrier and as an encapsulating material. In present study, zein was extracted from five different indigenous maize varieties by using three different concentration of ethanol (60%, 75% and 90%). The extracted zein was characterized to check its functional properties, antioxidant activity and antimicrobial activity. Zein is used for encapsulation of bioactive compounds and essential oils. Ginger oil was encapsulated in zein and zein/gum Arabic combination. Ginger oil has been used for many times as medicine. Its compounds are important for curing different diseases especially gastrointestinal diseases. The encapsulated material was then characterized by different analysis for structural properties and morphological properties.Controlled release of oil from nanocapsules was also determined. The encapsulated material was used in product development to evaluate its availability. The product was analyzed for different parameters and its dough rheology was also checked. It was stored for two months. All the data obtained was statistically analyzed. Agaiti-2002 was found best among these five varieties as it has higher protein and fibre content. In mineral profile zinc was found higher in this variety and zein extraction was also found maximum in this variety at all three levels of ethanol percentage and it may be due to high protein percent in this variety. The zein extracted from this variety was also higher in antioxidant potential and functional properties which included foaming properties and emulsifying properties. So Agaiti-2002 was selected for zein extraction and further analyses of zein. Zein/ ginger oil nanoparticles were produced in size 200-500nm and no structural change occur in encapsulating material during production of nanoparticles. After reconstituting lyophilized nanoparticles powder dispersions prepared at different pH which presented the steady release of ginger oil at a lower pH in minimum time. Zein and zein/gum Arabic combination used in production of bread by supplementing flour and significant improvement in physical dough characteristics and internal characteristics of bread was seen.
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مولانا ابوالکلام آزاد

آہ! ترکش مارا خدنگ آخریں
واحسرتا!ابھی حضرت شیخ ؒکی جدائی کاغم تازہ ہی تھا اور امتدادِ روزگار کا مرہم اس غم کی جراحت سامانیوں کوکم نہیں کرسکا تھا کہ اچانک مولانا ابوالکلام آزاد کی وفات حسرت آیات کاسانحۂ جاں گداز پیش آگیا۔اناﷲ واناالیہ راجعون۔
خامہ انگشت بدنداں کہ اسے کیا لکھیے
ناطقہ سر بگریباں کہ اسے کیاکہیے
عام پیرایۂ بیان میں لوگ کہتے ہیں کہ مولانا علوم قدیمہ وجدیدہ کے مبصر عالم تھے۔سحر طراز انشاء پرداز،بلند پایہ ادیب، جادو فشاں خطیب تھے۔فہم و تدبر، ذہانت وفطانت اُن کے اوصاف وکمالات ِطبعی کاتکمۂ زریں تھے۔لیکن حقیقت یہ ہے کہ یہ سب عنوانات مولانا کی اصل شخصیت کی ترجمانی اورعکاسی سے قاصر ہیں وہ بذاتِ خودایک تاریخ تھے اورتاریخ ساز بھی، وہ مستقل ایک عہد تھے اور عہد آفریں بھی۔انھوں نے اپنے دماغ اورزبان وقلم سے ایک عہد پیدا کیاجس کی ہمہ گیری اوروسعت کایہ عالم تھا کہ اُ س سے مذہب بھی متاثر ہوااورادب بھی، سیاست بھی اُس سے اثرپذیرہوئی اورتہذیب وثقافت بھی۔اُن کاقلم ابرِ بہاراں بھی تھا اوربرقِ شررفشاں بھی۔علم وحکمت اورشعروادب کے میدان کی طرف نکل گیاتوفروغِ نظراورایمان ویقین کے لالہ وگل کوپیغام ِنشوونما دیتا گیا اورچٹیل میدانوں کوگل وگل زاربناگیا۔اوراگراُس نے مذہب اورسیاست کی طرف رخ کیاتوفکر ونظر اوراحساس وشعور کی دنیا میں طوفان برپا کرگیا،جوگوشہ نشین تھے وہ اس آواز کوسن گھروں سے نکل پڑے ،جن پر غلامی کی غفلت ومدہوشی کاتسلّط تھا وہ جوش وولولۂ عمل سے سرشار ہوکرزندگی کاایک نیا خون اپنی رگوں میں دوڑتاہوا محسوس کرنے لگے۔وہ ہر بزم اورہر محفل میں پہنچا اورہرجگہ صدر انجمن اورمیر محفل ہوکررہا۔مذہب،سیاست اور ادب،تہذیب اورمعاشرت ان میں کوئی منزل ایسی نہیں ہے جس کی طرف اُ س نے رخ نہ کیاہو اوراُس میں اپنے اجتہاد وتحقیق ،سنجیدہ فکراور نظر بلند کے لازوال نقوش نہ چھوڑ گیاہو۔
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