Secondary metabolites, substantially available in the medicinal plants, have divulged their pharmacological properties and can be used in its isolated form or as integral component of the plant‟s part, to cure a variety of disorders. High blood pressure is considered as silent killer as it sneaks up in the body and may leads to serious cardiovascular disorders. Many allopathic antihypertensive medicines are presently available but these are taking their toll in the form of serious side effects. Therefore, the present study was designed for extraction and evaluation of plant‟s secondary metabolites as green Angiotensin Converting Enzyme (ACE) inhibitors for management of hypertension. In the first phase of this research, aqueous and ethanol extracts of 22 plants were initially screened as green ACE inhibitors. Among them four plants Coriandrum sativum, Amomum subulatum, Rauvolfia serpentina and Curcuma longa showed highest ACE inhibition potential. Secondary metabolites were extracted from these four medicinal plants and evaluated for ACE inhibition potential. The highest ACE inhibition potential was observed with flavonoid (81.4±0.48%) of Coriandrum sativum, tannin (77.9±0.24%) of Amomum subulatum, flavonoid (79.9±0.42%) and tannin (88.3±0.26%) of Rauvolfia serpentina and alkaloid (44.4±1.32%) of Curcuma longa. Plants are traditionally recognized for their synergic therapeutic effects, therefore, combinations of plants and their secondary metabolites were evaluated for synergistic ACE inhibition potential. The results revealed that the combination No.1 comprising of Rauvolfia serpentina and Curcuma longa (RS+CL) exhibited significant ACE inhibitory activity (65.08±0.33%) with IC50 value of 73.67μg/mL. The combination No. 13 of secondary metabolite comprising of tannins and flavonoids of Rauvolfia serpentina and alkaloids of Curcuma longa (TRS+FRS+ACL) showed highest ACE inhibition potential (69.64±0.80%) with IC50 value of 39.67μg/mL. Secondary metabolites present in combination No. 13 were further fractionated through column chromatography. Different fractions of flavonoids, tannins and alkaloids were collected, but among them only F3 fraction of flavonoids, T3 fraction of tannins of Rauvolfia serpentina and A6 fraction of alkaloids of Curcuma longa showed ACE inhibition potential. In 2nd phase of the study, characterization of five secondary metabolites fractions extracted from four selected medicinal plants and secondary metabolites present in combination No. 13 were performed by LC-ESI-MS/MS technique to find out the actual bioactive compounds responsible for ACE inhibition potential. The ACE inhibitors identified from flavonoids fraction were included pinocembrin, apigenin, pseudobaptigenin, quercetin, myricetin-3-O-glucoside, quercetin- dimethyl ether-O-glucuronide, quercetagetin, Luteolin-7-O-glycoronyl and quercetin-3-O-hexose-pentoside. Tannins fraction contained ellagic acid, megiferin gallate, prodelphinidin B4, tri galloyl glucose and geraniin as ACE inhibitors. Pyrazolo[1,5-a]pyridine,3,3a,4,7-tetrahydro-3,3-dimethyl;(3aS) and 2-(2‟-methyl-1‟-propyl)-4, 6-dimethyl-7-hydroxyquinoline were identified as alkaloidal ACE inhibitors. In the 3rd phase of this study, the combination No. 1 of plant (RS+CL) and combination No. 13 of secondary metabolites (TRS+FRS+ACL) were investigated for in vivo antihypertensive potential through spontaneous induction of hypertension by two kidney one clip (2K1C) renal artery ligation method. In vivo trial revealed that the combination No. 13 of secondary metabolites showed comparatively better antihypertensive potential as compared to the combination No.1 of whole plants. It is pertinent to mention that the antihypertensive potential of the isolated secondary metabolites was better even than the standard drug (Captopril) which was used as reference.
شعیب قریشی افسوس ہے کہ گزشتہ مہینہ متحدہ ہندوستان کی ایک اہم شخصیت شعیب قریشی صاحب نے کراچی میں انتقال کیا وہ ہندوستان کے ممتاز رہنماؤں میں تھے، ان کی عمر کا بڑا حصہ ملک و ملت کی خدمت میں گزرا ان کی قومی زندگی کا آغاز مشہور انگریزی اخبار ’’نیوایرا‘‘ کی مضمون نگاری سے ہوا تھا، جس کے وہ بعد میں ایڈیٹر بھی ہوگئے تھے، پھر ۱۹۱۴ء میں جنگ بلقان کے زمانہ میں مشہور انصاری مشن کے ساتھ ٹرکی گئے، ۱۹۲۰ء میں جب وفد خلافت لندن گیا تھا تو وہ وہاں زیر تعلیم تھے، اس لیے وفد کی کاروایوں میں عملی حصہ لیا، اسی زمانہ میں خلافت اورکانگریس کی تحریکیں شروع ہوئیں۔ لندن کی واپسی کے بعد ان میں سرگرمی سے حصہ لیا، ایک عرصہ تک خلافت کمیٹی کے سکریٹری بھی رہے، اور کچھ دونوں گاندھی جی کی غیر حاضری میں ان کے اخبار ’’ینگ انڈیا‘‘ کے بھی ایڈیٹر رہے، حجاز پر سلطان عبدالعزیز آل سعود کے قبضہ کے بعد وہاں کے حالات کی تحقیقات اور اس کے آئندہ نظام حکومت کے بارے میں مشورہ دینے کے لیئے خلافت کمیٹی کی جانب سے جو وفد گیا تھا اس کے ایک رکن شعیب قریشی بھی تھے، پھر نہرو کمیٹی میں مسلمانوں کی نمائندگی کی، مگر اس کی رپورٹ سے انھوں نے اختلافات کیا اور سیاسی زندگی سے کنارہ کش ہوکر ریاست بھوپال میں وزیر تعلیم ہوگئے، اپنے دورِ وزارت میں انھوں نے بہت سے مفید تعلیمی اور مذہبی کام انجام دیئے، ریاست بھوپال کے خاتمہ کے بعد پاکستان چلے گئے، اور مختلف اوقات میں مرکزی حکومت کی وزارت اور سفارت کے عہدوں پر ممتاز رہے، علمی قابلیت اور سیاسی بصیرت کے ساتھ اخلاقی حیثیت سے بھی بہت بلند اور پختہ کیرکٹر کے انسان تھے، ادھر چند سال سے انکا ذکر سننے میں نہیں آتا تھا، ایک دن...
If you have a relationship with the past, a sustainable plan can be done for future. Islamic personalities, especially the introduction of the scholars, intensify the path of progress in the field of scientific and research as well as the continuous hard work in the practical world. Similarly, when introduction of the people with historical roles have reached to the next generation, it is easy to define pathways and take lessons from the opponents to work hard for them. Introducing individuals with a specific region is worth a king's ransom investment for all its geographically entrusted and external roles and a leading rule for a new generation of races. In the underlying article, efforts are being made to present brief introduction of the scholars of the District Haripur who have written in various sciences and fictions, so that people of this region can know that this area also remained the base of Islamic scholars. Due to this article, depicting the introduction of the scholars of different school of thoughts will undermine an enormous and sectarian ideology, and the strength of tolerance and brotherhood will be strengthened.
The study engaged six secondary chemistry teachers in analysis of text and visuals of some selected topics of chemical bonding and acids and bases in Sindh Textbook Board’s grade nine chemistry textbook. The purpose of analysis was to explore gaps in the selected text and visuals. Along with analysis teachers’ views about improvement of the selected text and visuals were also collected. Furthermore, the study explored students’ difficulties with the selected text and visuals. The study used qualitative exploratory study design. The data collection tools used were questionnaires and focus group interviews. Separate questionnaires for teachers and students were developed by the researcher. The study engaged six chemistry teachers from four different schools of Karachi (three government and one private) and twelve grade nine students from another government girls’ high school of Karachi. The major findings from teachers revealed that some text of the selected concepts in the textbook lacked adequate explanation. These concepts are: (a) introduction to chemical bond formation (b) electrostatic nature of covalent bond (c) development of charge on ions (d) limitations of Arrhenius and Lowery-Bronsted models of acids & bases (e) some scientific terms like delocalization, concept of litmus etc. The study also found that the explanation of the selected concepts given in text lacked inclusion of students’ previous knowledge and analogies. It was also discovered during the study that absence of sub-microscopic level and macroscopic level diagrams about given chemical reactions of acids and bases made the concept difficult for the students. Students were also found struggling with dot and cross diagrams and high use of scientific language in text. The study recommends textbooks writers to include the important information and explanations of the concepts in the text that has been found missing during this study. Moreover, it is also recommended to textbook writers that incorporation of students’ previous knowledge, analogies, macroscopic and sub-microscopic level visuals about chemical equations is made in the text. Similarly, textbook writers are recommended to use simple language in text for explaining chemical concepts rather than using a lot of scientific terms. Furthermore, teachers are recommended to provide that important content to students during teaching which is missing in textbook.