Search or add a thesis

Advanced Search (Beta)
Home > Extraction of Bioethanol from Agricultural Wastes by Using Termites Gut Flora Through Fermentation

Extraction of Bioethanol from Agricultural Wastes by Using Termites Gut Flora Through Fermentation

Thesis Info

Access Option

External Link

Author

Batool, Iram

Program

PhD

Institute

Pir Mehr Ali Shah Arid Agriculture University

City

Rawalpindi

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9044/1/Iram_Batool_Biochemistry_HSR_2018_PMAS_18.04.2018.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726176778

Similar


Pakistan utilizes huge amount of budget to import petroleum products from other countries because very limited resources of fossil fuels are available in country. However combustion of fossil fuel increases concentration level of greenhouse gases, those are destroying environment. There is a need of some alternate and cheaper resources of energy to overcome the deficiency of fuels. There are number of challenges for the marketable applications of the advanced technologies of fuel production which includes pretreatment methods and enzymes production. For the microbiologist and ecologist, termite gut represents an outstanding representative of highly structured micro environments and therefore also called as mini biorefineries. Because the decomposition of lignocelluloses is proficiently perform by these soil insects. A study was conducted to develop a method for the production of bioethanol from agricultural waste materials which includes corn stover, cotton stalk and rice straw by using cellulolytic bacteria from termite gut. Bacteria were isolated from termite gut and identified by 16S rRNA gene sequencing. The isolates were screened for CMCase and xylanase activity. Three isolates were selected among which two were bacillus sp. and the one was cellulomonas sp. Their avcilase CMCase and xylanase activities were studied on selected temperature and pH. It was found that all of the bacterial isolates have shown higher avicelase activity at 40 oC, CMCase and xylanase activity at 50 oC. Isolate 9x, 10 and 31 have shown higher avicelase activity at 7 pH, whereas CMCase activity was found to be optimum at pH 8. In case of xylanase activity isolate 9x and 10 showed higher activity at pH 6 and isolate 31 at pH 7. Biological and chemical pretreatments were compared for corn stover, cotton stalk and rice straw. Efficiency of enzymes of bacterial isolates for saccharification of agricultural substrates was assessed. It was observed that xylanase (isolate 9x) 1 2 released more sugar amount from corn stover as compared to other substrates. Direct saccharification and fermentation of corn stover and rice straw was carried out using all three bacterial isolates without using pretreatment methods. Ethanol concentration was analyzed by using High performance Liquid chromatography. It was observed that isolate 31 has produced higher amount of ethanol by using corn stover. Therefore it was found that these bacterial enzymes have the potential to hydrolyze not only pure substrates but can also degrade agricultural substrates. Bacterial isolates obtained from termite gut have potential to can convert different agricultural substrates into valuable bioproducts including various alcohols (ethanol) which may have new innovative source for producers.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

جسٹس مولاناسید کرامت حسین

جسٹس مولانا سیّد کرامت حسین
جسٹس سید کرامت حسین کی ناگہانی موت گو عام دنیائے علم کے لئے کچھ کم باعث حسرت نہیں ہے لیکن ہمارے لئے اس سے زیادہ غم افروز ہے۔ مرحوم ہماری مجلس کے نائب صدر تھے اور ہمیشہ اپنے قیمتی مشوروں سے ہماری اعانت کرتے تھے۔ وہ خود بھی علمی مشاغل میں مصرف رہتے تھے، آخر عمر میں ’’المرآۃ‘‘ نام ایک ضخیم کتاب عورتوں کے حقوق و خصائص پر تصنیف فرما رہے تھے۔ ان کی سادگی اخلاص کار، ایثار اور خالص علمی خدمات ہمیشہ یادگار رہیں گی۔وہ لکھنؤ کے خاندان اجتہاد سے تھے، انہوں نے عربی کی تکمیل کے بعد انگریزی کی طرف توجہ کی اور لندن جاکر بیرسٹر ہوئے، انکو فلسفہ سے خاص ذوق تھا، جدید فلسفہ کے دقائق کو اردو میں لکھنے کی ابتداء انہیں سے ہوئی، سالمات کی اصطلاح انہیں کی بنائی ہوئی ہے، اردو میں افراد کاسیہ کے نام سے ان کا بڑا اچھا رسالہ ہے، وہ ہندوستان واپس آکر علی گڑھ کالج میں پہلے قانون کے پروفیسر ہوئے۔ اسی زمانہ میں مولانا شبلی سے ان کی ملاقات اور راہ و رسم ہوئی۔ عربی فلسفہ یعنی عربی فیلالوجی سے ان کو بڑی مناسبت تھی۔ المقدسہ کے نام سے عربی میں ان کا ایک رسالہ نہایت مفید ہے۔ آخر میں الہ آباد ہائیکورٹ میں جج ہوگئے تھے۔ اس سے الگ ہونے کے بعد لکھنؤ میں قیام کیا تھا۔ مسلم گرلز اسکول انہیں کے وقف سے وجود میں آیا۔
اس زمانہ میں دارالمصنفین نیا نیا قائم ہوا تھا ہمارے ارکان خاص میں سے مولوی عبدالماجد صاحب دریابادی اور مولوی عبدالباری صاحب ندوی، جدید فلسفہ کے عشاق میں تھے اور اس وقت ان کے نزدیک سب سے بڑا کام یہ تھا کہ اردو زبان میں جدید فلسفہ کی اہم کتابوں کو منتقل کیا جائے، اس بناء پر جسٹس سید کرامت حسین صاحب سے اس...

موجودہ معاشرتی اضطراب اور اس کا حل: سیرت طیبہﷺ کی روشنی میں

Social anxiety is the fear of interaction with other people that brings on self-consciousness, feelings of being negatively judged and evaluated, and, as a result, leads to avoidance. Social anxiety is the fear of being judged and evaluated negatively by other people, leading to feelings of inadequacy, inferiority, embarrassment, humiliation, and depression. The major causes of Social anxiety are Rights abuses, Provocation, corruption, murder, Law-lessens, nepotism, Prejudices, grouping, Propaganda and carelessness. All of these things are causes of the destruction of a prosperous society. Because of these each individual of society remains restive and society become victims of violence. In this paper the social anxiety conditions and its solution will be described in detail in the light of seerah.

Mor Rpho-Gen Netic an Nalysis and Ex S Xploitat Tion of He Eterosis Among Hip Be S G Earing H Hybrid R Roses

Roses have contributed to our social, economic and religious life hence they are called “Index of Civilization”. Presently in Pakistan most of the rose cultivars have been introduced from other countries hence they fail to fit in new environmental conditions of the country. Keeping in view the importance of this issue present study was aimed to get insight into the breeding procedure, complexities and tactics leading to achieve hybrid progenies and to study the magnitude of hybrid vigor inherited in the next generation of parent roses. For this purpose, comparatively well acclimatized and hip producing cultivars including “Autumn Sunset”, “Iceberg”, “Paradise”, “Angel Face”, “Casino”, “Louise Odier”, “Grand Margina”, “Handel” and “Gruss an Teplitz” were selected for crossing. They were planted in the Rose field of Institute of Horticultural Sciences, university of Agriculture, Faisalabad, Pakistan. Morphological description of all cultivars revealed various blends of color of flowers, foliage and leaf hairiness, petiole pubescence and inflorescence types. Data regarding flowering and yields traits also varied in all varieties, however over all better performance was exhibited by the cultivars Iceberg and Gruss an Teplitz scoring 2.5 points each. Poor performance was exhibited by the cultivar Louise Odier. The genotypic and phenotypic and environmental coefficients of variations varied for all quantitative traits. Strong positive correlation was exhibited between number of flowers per plant and overall performance of bushes, flower diameter and petal length, bush shape and height of the plant however fragrance was negatively correlated to flower persistence life. Cluster analysis of the parent on the basis of quantitative traits revealed four major clusters which predicted the different performance for some traits but same for other. Within the individual cluster all the cultivars almost behaved similar. Results about pollen vigor parameters indicated that great variations in pollen quality, quantity, and viability and germination % ages. Maximum number of pollens per anther were exhibited by the cultivar Handel (1612.3) followed by cultivar Angel face (1588.7). Minimum numbers of pollens per anther were counted in the cultivar Iceberg. On average basis the performance of Angel face and Handel was better with respect to both number of anther per flower and number of pollens per anther. Pollen size (L: D) was almost same in all cultivars hence no significant differences were observed. Comparison of means of viability %age revealed that maximum number of viable pollens in term of viability %age were possessed by cultivar Handel (70%) followed by ‘Gruss an Teplitz’ (64%). The minimum viability of pollens was exhibited by the Cultivar ‘Autumn Sunset’ having pollen viability of 35%. Germination medium having 15% sucrose proved very effective for optimum germination results as compared to 10% and 20% sucrose. On average basis the cultivar ‘Grand Margina’ yielded maximum pollen tube length of 46.556 μm followed by ‘Gruss an Teplitz’ having pollen germination of 40.333 μm. The minimum pollen tube length of 9.222 μm was observed in cultivar ‘Iceberg’. Cluster analysis of cultivars on the basis of pollen quality and vigor parameters revealed three main groups. A strong correlation among pollen germination %age and pollen tube length (r = 0.77) and pollen germination %age and pollen viability %age (r = 0.731) was exhibited. Pollen germination is also strongly correlated with pollen size (r =0.738) The comparison of various fertility parameters in vitro and in vivo revealed that cultivar “Handel” showing its maximum pollen viability in vitro performed well with respect to giving more crossing success in field as compared to its average pollen germination %age invitro conditions followed by the cultivar Autumn Sunset. Cultivar Ice Iceberg did not yielded any successful cross. Maximum hip setting %age (83) was observed in for the cross combinations Iceberg x Gruss an Teplitz and Iceberg x Casino and minimum (30%) in Louise Odier x Paradise. Number of seed per hip also varied from 33 to 15 in all cross combinations. Response of seeds with respect to germination also varied significantly after treating the seed with various storage temperatures and acid treatments. Results indicated that germination period (F= 89.95, F<0.05), germination %age (F=67.16, P<0.05), imbibition period (F=26.13, P<0.05) and seed vigor index (F= 112.2, P<0.05) was affected significantly by all treatments accordingly. All the treatments also affected morphological traits of seedlings as well. Evaluation of hybrida in the field revealed that all the F1 hybrids produced showed significant variations in qualitative and quantitative traits over their parents. Data regarding heterosis and heterobeltiosis %ages showed also negative and positive results for various traits over their parents. Variations were observed for flower color and diameter, prickles, bush shape and overall performance of all F1 hybrid progenies. Performance of F1 progenies was evaluated in the field conditions while having comparison with their respective parents and data was analyzed by ANOVA to check the significance of variations in the parents and progenies and comparison of means. Further, more hybrid vigor estimation (Heterosis and Heterobeltiosis) proved a useful tool to check the superiority and inferiority of progenies over their parents. Cluster analysis of the parent cultivars and progenies was useful to develop realation on the basis of similarity index among parent and hybrid progenies. Hybrid identification and genetic diversity studies among parents and progenies evaluated by SSR markers also confirmed the contribution of both parents for the development of F1 hybrids. For genetic diversity study SSR loci proved very effective to amplify across parent cultivars, hybrids and to assess their polymorphic contents. Ten of the SSR primers exhibited similar sequence with known gene. Estimates of genetic diversity varied remarkably among loci. Observed heterozygosity (Ho) based on Nei’s estimation revealed that the locus H10, CL9 and RW54 contributed equally for generating more variation and scored maximum number of alleles for respective locus. Allele fixation trends were also observed for different loci and individual groups. Total gene diversity (Ht) was calculated and mean for all loci was 0.852 while the average gene diversity (Hs) for all loci was calculated as 0.768. It was clear from the dendrogram that parent cultivars compiled themselves into four main groups. Little differences were observed within the groups of parent cultivars. Confirmation of hybrids was done by comparing the common alleles between parent and hybrid against all primers. More over phenatic relationship was developed among hybrid progenies and parents to confirm the extent of contribution of each parent for genetic makeup of hybrid. All other hybrids were identified in the same method. It is concluded from the present study that hybrid cultivars have varying level of fertility and it is necessary to evaluated them in search of vigorous pollen donor parent. It is concluded from the result that diallel was not possible as most of the crosses failed. However ANOVA, comparison of means and Cluster analysis aided by SSR fulfilled the objective. Hybrid progenies exhibited vigor over parents for some traits but for other traits they remained uneffective in the climate of Faisalabad city. SSR markers proved helpful tool for confirmation of F1 hybrids and discriminating the parent cultivars and progenies.