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Home > Fasciolosis in Pakistan: Adult and Egg Phenotyping, Risk Mapping and Serological Studies in Sub-Tropical Punjab

Fasciolosis in Pakistan: Adult and Egg Phenotyping, Risk Mapping and Serological Studies in Sub-Tropical Punjab

Thesis Info

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Author

Kiran Afshan

Program

PhD

Institute

Pir Mehr Ali Shah Arid Agriculture University

City

Rawalpindi

Province

Punjab

Country

Pakistan

Thesis Completing Year

2014

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1574

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726198179

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Fasciolosis also known as fascioliasis is a highly pathogenic liver infection caused by F. hepatica and F. gigantica. The study was aimed to use morphological markers for identification of fasciolids, generation of risk maps and use of rapid and cost effective diagnostic tool to reduce the impact of fasciolosis on animal health. The phenotypic features of adults and eggs of fasciolid species infecting buffaloes from central Punjab area, Pakistan, have been studied to characterize the fasciolid populations involved. Morphometric analyses were made by applying standardized measurements with a computer image analysis system (CIAS). The current investigation is first time conducted in Pakistan to confirm the taxonomic status of fasciolids by comparing with other pure standard populations viz., F. hepatica of European Mediterranean origins and F. gigantica representing Burkina Faso (Africa). In these geographical areas there is no overlapping of both fasciolids species. Only parasites obtained from bovines were employed. The climatic factors influencing fascioliasis presence and potential spread were analyzed from five meteorological stations during 1990-2010. The fascioliasis forecast risk Mt and Wb-bs (Water-Budget-Based System) indices and NDVI (Normalized Difference Vegetation Index), known to be useful for fascioliasis assessment, were obtained and correlated with geographical distribution, seasonality patterns and two-decade evolution of fascioliasis in livestock throughout the province. These two climatic forecast indices and a remote sensing marker are used to characterize the climatic factors and the earth surface in order to ascertain the epidemiological complexity and time-lag dynamics of fascioliasis. The seroprevalence of fascioliasis was also determined in sub-tropical Punjab with the application of a very sensitive and xxxispecific ELISA test by using monoclonal antibodies which are able to detect even very low intensity infection. The MM3 Sero-ELISA was applied to check the status of fascioliasis. The increase of disease transmission risk in the lowlands should be highlighted, given that the largest part of the Punjab province includes low altitude, highly irrigated plains. The importance of livestock in this province makes this phenomenon to be given forecast priority assessment henceforth in order to establish the adequate control measures. The use of cost effective diagnostic tools would be helpful to reduce the impact of fascioliasis on animal health by selecting the appropriate anthelmintic treatment. An annual treatment scheme to effectively control the disease is finally recommended to be applied throughout the whole Punjab province.
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مولانا مفتی عتیق الرحمن

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۱۲؍ مئی ۸۴؁ء کو ہندوستان کے نامور عالم مولانا مفتی عتیق الرحمن فالج کے موذی مرض میں ایک طویل مدت تک مبتلا رہ کر ۸۴ سال کی عمر میں اس دارفانی سے رحلت گراے عالمِ جاودانی ہوئے۔
ان کے اس مرض کی المناکی دارالمصنفین سے بھی بڑی حد تک وابستہ ہے، اس لئے اس ادارہ کے خدام ان کی وفات حسرت آیات سے بہت سوگوار ہیں، فروری ۱۹۸۲؁ء میں یہاں ’’اسلام اور مستشرقین‘‘ پر جو سیمینار ہوا تھا، اس میں وہ شرکت کے لئے تشریف لائے تھے، تین روز یہاں بہت ہنسی خوشی سے گذارے، اس کے ایک اجلاس کی صدارت بھی کی، خوش خوش یہاں سے اور شرکاء کے ساتھ روانہ ہوئے تو ریل ہی میں بارہ بنکی کے پاس ان پر فالج کا سخت حملہ ہوا، ان کے ہم سفر مولانا سعید احمد اکبر آبادی اور مولانا سجاد حسین نے ان کو کسی طرح لکھنؤ کے ہسپتال میں داخل کیا، ان کی طبیعت کچھ سنبھلی تو پھر دہلی لے جائے گئے، اس وقت سے اپنی وفات تک تقریباً سوا دو سال تک بستر ہی پر رہے، خیال ہوتا ہے کہ وہ دارالمصنفین کا سفر نہ کرتے تو اس موذی مرض میں مبتلا نہ ہوتے، مگر مشیتِ ایزدی یہی تھی، راقم ان کی عیادت کے لئے کئی بار ان کی خدمت میں حاضر ہوا ان کا جسم تو بیکار ہوچکا تھا، مگر دماغ بیدار رہا، گفتگو میں وہی روانی اور شیر ینی ہوتی جو ان کی طبیعت کا مخصوص رنگ تھا، ہر قسم کے مسائل پر گفتگو کرتے، مگر زیادہ تر دارالعلوم دیوبند کے قضیۂ نامرضیہ پر اظہار خیال کرتے، ایسا معلوم ہوتا کہ وہاں کا المیہ پیش نہ آتا تو اس مرض میں مبتلا نہ ہوتے اور ہوتے بھی تو اتنے دنوں تک بستر علالت پر پڑے نہ رہتے، دارالعلوم دیوبند...

Social Capital’s Impact on Civic Engagement: An Empirical Study on Pakistani Youth

The aim of this research is to assess the impact of social capital on civic engagement. The quantitative method was applied to measure impact of independent variables on dependent variable. The survey instrument was applied to collected data from undergraduate student of four general Universities of Pakistan. The partial least squares structural equation modeling (PLS-SEM) approach was applied to measure impact of bridging, bonding social capital and trust on civic engagement. Results indicate that bonding social capital and trust have strong association with civic engagement, however, association between bridging social capital and civic engagement was found insignificant. The analyses indicate that it is a basic requirement to bring immediately policy reforms in context of youth development and create more opportunities for youth to engage in the social and structural development of society.

Cotton Gossypium Hirsutum L. Yield and Quality under Different Inputs and Crop Establishment Techniques in a Wheat-Cotton System

Wheat (Triticum aestivum L.) – cotton (Gossypium hirsutum L.) system is a major production system in Pakistan, however, cotton yield and quality is declining in the system most likely due to weeds infestation, nutrient stress, population densities (either too low or too high) and intensive tillage practices. Conservation tillage system has been introduced to avoid late sowing of wheat and to reduce the land preparation cost in wheat-cotton system. Conservation tillage such as reduced and zero tillage in combination with appropriate herbicide, optimum N and intra-row spacing may have the potential to enhance cotton yield and quality on sustainable basis. The objective of this study was to determine the effect of post-emergence herbicides, nitrogen rates and intra-row spacing under different tillage systems on cotton yield and quality in wheat-cotton system. Three field experiments were conducted at Cotton Research Station, Ratta Kulachi, Dera Ismail Khan, Pakistan, during 2010 and 2011. In experiment 1, post-emergence herbicides, i.e. haloxyfop-R-methyl 10.8 EC (108 g a.i. ha-1), lactofen 24 EC (168 g a.i. ha-1), haloxyfop 10.8 EC + lactofen 24 EC, hand weeding, weedy check and three tillage systems [zero tillage (ZT), reduced tillage (RT) and conventional tillage (CT)] were studied. In experiment II, five nitrogen levels (0, 50, 100, 150, and 200 kg N ha-1) and three tillage systems (as in experiment I) while in experiment III, four intra-row spacing (15, 22.5, 30, 37.5cm) and three tillage systems (as in experiment I) were evaluated in randomized compete block design (RCBD), with split plot arrangements, replicated 4 times. Tillage was allotted to main plots in all the experiments while herbicides, N rates and intra-row spacing were applied to subplots in experiment I, II and III, respectively. Mean of the two years data revealed that broad spectrum herbicides (Haloxyfop + Lactofen) and hand weeding reduced weed density by 78 and 93 %, respectively, compared to all other weed control treatments. Interaction effects revealed that RT in combination with broad spectrum herbicides had maximum weeds reduction percentage (95%). RT with broadspectrum herbicides had higher plant height, more bolls plant-1, boll weight, ginning out turn, seed cotton yield, fiber length, fiber strength and fiber fineness compared to ZT and CT. It is concluded that broad-spectrum herbicides under RT produced higher cotton yield and fiber quality in wheat based cropping system on silty clay soil of D.I.Khan. In experiment II, nitrogen (N) and tillage interaction is of great economic importance to improve crop yield and N use efficiency. Nitrogen rates had significant differences for yield components and seed cotton yield. Nitrogen rate of 150–200 kg N ha-1 produced highest seed cotton yield due to more bolls plant-1 and greater boll weight. The lower N rate (50-100 kg ha-1) produced the optimum nitrogen agronomic efficiency (NAE, 3.5-4.0 kg kg-1), nitrogen physiological efficiency (NPE, 11-12 kg kg-1), and nitrogen recovery efficiency (NRE, 32-33 %) than higher N rate (150-200 kg ha-1). Tillage × nitrogen interactions revealed that RT had greater bolls plant-1, boll weight, GOT, fiber length, and strength at 150–200 kg N ha-1 compared to other tillage system. The micronaire revealed no difference among tillage systems in fiber fineness. Using 150–200 kg N ha–1 in conservation tillage may be a sustainable approach to enhance cotton yield (3549 kg ha-1) and quality. In experiment III, intra-row spacings significantly affected yield, yield components and quality in both years. The highest seed cotton yield was obtained from 22.5 cm intra-row spacing. Interaction effects revealed that RT × 22.5 cm intra-row spacing produced optimum seed cotton yield. The ginning out turn and fiber strength was higher in RT × 22.5 cm intra-row, except fiber length. In conclusion, RT at 22.5 cm plant to plant spacing produced maximum seed cotton yield (4060 kg ha-1). The overall results suggest that using broad-spectrum herbicides along with reduced tillage, 150–200 kg N ha–1 at a row swpacing of 22.5 cm constitutes a near-optimal system of cotton management in the wheat-cotton system of Dera Ismail Khan.