Sugarcane is a tropical plant (Humbert, 1968) and requires warm cum humid climate for its good growth. However, it is being grown over the sub tropics land surface of earth between latitude 30o N and 35o S (Nazir, 1994). Pakistan lies in sub tropics with semi arid climatic conditions where sugarcane is grown on an area of 1.241 million hectares with a total annual stripped cane production of 63.92 million tonnes giving an average stripped cane yield of 51.51 t ha-1 (Govt. of Pakistan, 2008). Two field experiments were conducted for two consecutive years during 2006 and 2007 at Farm area, Sugarcane Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan, to explore the production potential of spring planted sugarcane at higher levels of N and K, evaluate fertilizer application timing and to improve the nutrient (N & K) use efficiency. The experiments were laid out according to Randomized Complete Block Design (RCBD) having three replications with a net plot size of 6 m x 8 m. Results revealed significant effect of dose and time of nitrogen application on all agronomic and physiological traits except germination, number of shoots, number of internodes, NAR, HI and all quality parameters which were statistically non significant. The maximum stripped cane yield (108 & 101 t ha1) and sugar yield (12.8 & 12.1 t ha1) were recorded at T7 (126kg N ha-1 at sowing + 126 kg N ha-1 at 90 DAS) during 2006 and 2007, respectively. K2O level and time of its application has significant effect on all agronomic, physiological and quality parameters except germination, number of shoots, plant height, number of internodes, NAR, HI, fiber %, cane juice % and weight of bagasse % during both the years. The maximum stripped cane yield (116 & 107 t ha-1) was recorded at T7 (84 kg K2O ha-1 at sowing + 84 kg K2O ha-1 at 90 DAS) during 2006 and 2007, respectively. Sugar yield (14.7 t ha-1) was maximum at T7 (84 kg K2O ha-1 at sowing + 84 kg K2O ha-1 at 90 DAS) in 2006, while it was highest (14.1 t ha-1) in T10 (112 kg K2O ha-1 at sowing + 112 kg K2O ha-1 at 90 DAS) during 2007.
ڈاکٹر نعیم احمد افسوس ہے کہ گذشتہ ماہ پروفیسر ڈاکٹر نعیم احمد ، صدر شعبۂ اردو علی گڑھ مسلم یونیورسٹی انتقال کرگئے، وہ بڑے خلیق ، ملنسار اور مرنجاں مرنج شخص تھے۔ ابھی وسط نومبر میں انھوں نے اپنے شعبہ میں توسیعی خطبہ دینے کے لیے مجھے مدعو کیا تھا مگر میں نے اپنی مشغولیت کی بنا پر اس وقت معذرت کردی تھی۔ اﷲ تعالیٰ ان کی مغفرت فرمائے۔ (ضیاء الدین اصلاحی، فروری ۱۹۹۶ء)
This study explores Western parameters and principles of freedom of expression from an Islamic perspective. Western thought advocates infinite freedom of speech but it also has some regulations, limits and restraints. Every Western human rights instrument and convention guarantee freedom of expression, but in fact, the excess of freedom is bringing the society to a harsh kind of clash and conflict. In simple words, freedom without sufficient legal and moral restrictions cannot be managed. It is also observed that without strict legal and moral boundaries, it cannot become fruitful for the state and society. Even the Western world, which is representative of maximum freedom of speech, also bounds freedom of expression with some parameters and restrictions. However, it is propagated that the Western laws provide outclass freedom of expression and speech. The actual situation is not as good as it is expressed, because on one hand, the international instruments provide freedom of speech and on the other, they limit it through certain laws. It means freedom of expression is not free of boundaries in the West also. In other words, it is only a mythology and controlled propaganda that the Western thought and civilization provides absolute and unlimited freedom of expression and press in their countries. Instead, Islamic teachings categorically agree to provide the right to freedom of expression but on the other hand, it binds them with some social, moral and legal boundaries to save the society from any kind of clash and conflict. It is perceived Western legal instruments limit freedom of expression with some legal boundaries and laws rather than ethics and morals. It is recommended on behalf of this study that the Western world also puts moral and religious boundaries on freedom of expression for its safety. An analytical & critical approach with qualitative research methodology is adopted in the study.
A number of analytical methods exist for the evaluation of oxidative stress. Some of these directly measure the accumulation of oxidizing species or of oxidized products, while other methods measure the defence capacity of the organism against oxidation, which is referred to as the specific or the total antioxidant capacity (TAC). Amongst these TAC methods, TEAC (Trolox Equivalent Antioxidant Capacity) assays which involve the use of radicals of compounds such as N,N-dimethyl-p-phenylenediamine (DMPD) or 2,2’-azinobis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS), have attracted the attention of biochemists and analysts due to their operational simplicity. These methods are based upon the spectrophotometric measurement of the antioxidant activity of body fluids, herbal extracts, fruits, vegetables and other food samples and have certain limitations with regard to their application range, stability and environmental fate. In the present study we have improved upon the validity/applicability of two already existing methods and explored two new and affordable decolorization assays with greater reproducibility and application range. At the cellular level, the effect of xenobiotics on E.coli, used as a model organism, has also been studied. An improved N,N-dimethyl-p-phenylenediamine (DMPD) radical cation decolorization method was developed for the measurement of the antioxidant activity in food and biological samples. The radical cation (DMPD .+ ) is generated through reaction between DMPD and potassium persulfate and is subsequently reduced in the presence of hydrogen-donating antioxidants. This assay has a clear advantage over the currently used version of the DMPD assay (the DMPD/FeCl 3 assay) on a number of counts. The chemistry involves the generation of a more stable DMPD radical monocation by a reaction with potassium persulfate. It does not involve Fe(II) ions for the generation of the radical cations, which through Fenton’s Reaction can cause negative deviations in the measurement of the antioxidant activity of biological samples. The new experimental procedure is fast, inexpensive and ensures sensitivity along with reproducibility in the measurement of antioxidant activity of hydrophilic and lipophilic compounds and thus promises use in the screening of large iABSTRACT numbers of fruit samples. The effectiveness of the new DMPD decolorization assay was verified by evaluation of antioxidant capacity of different citrus fruits. It was applied to the measurement of the antioxidant activity of a variety of extracts of fresh and stored seeds, peel and pulp of Grewia asiatica. The results were compared with those obtained by the ABTS radical cation decolorization assay. It was observed that the antioxidant activity of G. asiatica extracts is significantly affected when the samples are stored at 0 o C for the duration of one month. This may possibly be due to degradation and/or transformation of phenolic constituents of the extracts into other products. High TEAC values of fresh samples indicate potent in vitro antioxidant activity of G. asiatica extracts, which may also be related to its use in the traditional medicinal system against diseases such as diabetes mellitus, hepatitis etc. Statistically a positive correlation was found between quantity of phenolic contents and the antioxidant activity. The well known ABTS (2.2’-azinobis(3-ethyl benzothiozoline-6-sulfonic acid) radical cation decolorization assay, reported in hundreds of research papers, has been applied under different pH conditions for the determination of the TEAC of biological and food samples. Due to the innate chemical nature of ABTS ·+ , it is unstable at physiological pH. Further, its sensitivity is lost in an acidic medium. No single method was claimed to cover both the drawbacks of the ABTS procedure. An improved ABTS decolorization method was developed for the measurement of antioxidant activity of human plasma and herbal extracts with greater stability and sufficient sensitivity. A relatively stable ABTS radical cation was generated through reaction between ABTS and potassium persulfate in sodium acetate buffered solution at pH 6.5 (20 mM). The developed method was successfully applied towards the measurement of the antioxidant activity of plasma samples and extracts of Terminalia arjuna and Cuscuta reflexa. The modified assay proved to be highly reproducible. TEAC values for plasma samples ranged between 1.410-2.025 mM with a percent relative standard deviation (% RSD) of 1.64 and 1.72-1.98 and a mean % RSD of 1.27 for the already reported and the modified versions of the ABTS assay respectively. A novel, rapid and cost-effective Trifluoperazine dihydrochloride (TFPH) decolorization assay was developed for the measurement of the antioxidant activity of fruits that naturally exhibit a low pH. A chromogenic reaction between TFPH and potassium persulfate at low iiABSTRACT pH produced an orange-red radical cation with a maximum absorption at 502 nm in its first order derivative spectrum. A linear inhibition of color production was observed with linearly increasing amounts of antioxidants, with correlation coefficients (R 2 ) ranging from 0.999 to 0.983. The antioxidant capacities of synthetic solutions of standard antioxidants were evaluated by comparison with the standard inhibition curve using Trolox as the standard. Comparison of the antioxidant capacities determined using this newly developed TFPH assay and the ABTS-Persulfate decolorization assay not only proved the efficacy and sensitivity of the developed procedure but also yielded promising results for the determination of the antioxidant activity of a range of antioxidants found at a low pH. The newly developed assay is many times more cost effective and requires less time for the preparation of working solution than the ABTS assay. This suggests that the antioxidant activity of samples with a naturally low pH should preferably be evaluated through an assay in which the radical cation itself has been stabilized at low pH. Another new, quick and economical decolorization assay based upon the generation of a radical cation made from promathazine hydrochloride (PMZH) is described for screening of antioxidant activity of plants and herbal extracts. PMZH radical cations, which were produced through a reaction between PMZH and potassium persulfate in phosphoric acid medium, have a maximum absorption at 515 nm in their first order derivative spectrum. The concentrations of chromagen and potassium persulfate were optimized (final concentration of PMZH and K 2 S 2 O 8 were 0.166 mM and 0.11 mM respectively) for better stability and sensitivity of the radical cation produced. A good linear correlation was found between the inhibition of color formation and the increasing amounts of standard antioxidants, with correlation coefficients ranging from 0.989 to 0.999. The newly developed assay was employed to evaluate the antioxidant capacity of Citrullus colocynthis L. and Artemisia absinthium extracts. The proposed assay involved a more stable radical cation and required only 1 hr for preparation of a working solution as compared to the ABTS radical cation decolorizaion assay which was reported to be less sensitive at low pH and required 12-16 hours for preparation of the radical cation solution. Other assays also employed to evaluate the antioxidant potential and radical scavenging capacities of the extracts were the ferric reducing antioxidant power, 2,2’-diphenyl-1-picrylhydrazil radical scavenging, total phenolic iiiABSTRACT contents assay, the total flavonoid contents and metal chelating activity assays, and the lipid peroxidation value in linoleic acid emulsion systems. Extracts of medicinal plants have been reported to be very effective in the control of chronic diseases due to their high contents of antioxidants. Litchi chinensis Sonn. is used for its hypoglycemic activity in the traditional medicinal system of the Indian subcontinent. The antioxidant activity of the aqueous and organic extracts of leaves, stem and fruit pulp of L. chinensis was investigated using the 2,2’-azinobis(3-ethylbenzothiazoline-6-sulpohonic acid) (ABTS) decolorization assay, the ferric reducing antioxidant power (FRAP) assay, the 2.2’- Diphenyl-1-picrylhydrazil (DPPH) assay, the total phenolic content (TPC) assay and the total antioxidant activity assay. In the second part of research work, the actual response of cellular systems towards an artificially created stress environment was studied. E.coli was taken as a model organism and pesticides were used as the stress inducing compounds. Logarithmically growing batch cultures of Escherichia coli were exposed to sub-lethal concentrations of pyrethroid and carbamate pesticides of four different technical grades. This induced the formation of 17 to 20 stress proteins, as observed by two-demensional polyacrylamide gel electrophoresis. Two dimensional polyacrylamide gel electrophoresis was performed to resolve the proteins. Visualization of the protein spots by rapid silver staining resolved these 17-20 stress proteins which were absent in the standard protein profile of E.coli. On average 29% of these stress proteins were unique to each of the stress inducing agents, while the remaining number overlapped with those of other pesticides. The iso-electric points (PIs) and molecular weights of the proteins were determined by comparison with protein markers of known PIs and molecular weights. Furthermore, upon comparing the pesticide-induced proteins within the same class and between the two different classes (pyrethroid and carbamate), it was apparent that the gene or set of genes responsible for stress expression are also the same, irrespective of the chemical nature of the substituents of the pesticides. The analysis of stress proteins will aid the development of more sensitive techniques for pollutant analysis. The unique proteins could be purified to enable quick and sensitive detection of xenobiotics in the environment with an antibody based strip method." xml:lang="en_US