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Formulation Characterization and Non-Invasive In-Vivo Evaluation of Dermocosmetic Emulgels Containing Various Fruit Extracts

Thesis Info

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Author

Kashif, Muhammad

Program

PhD

Institute

The Islamia University of Bahawalpur

City

Bahawalpur

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Pharmaceutics

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12276/1/Muhammad%20kashif%20pharmaceutics%202019%20iub%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726229828

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Natural plant derived phytoconstituents with appreciable antioxidant activity have gained sufficient interest in cosmetic and dermatology for various clinical and aesthetic purposes. Various skin related disorders such as acne, inflammation, hyper-pigmentary disorders, sunburn, photoaging, melisma, and dry skin conditions are now becoming a point of focus to be treated with natural ingredients based topical formulations because of lower risk of adverse effects. In this research project bioactive, polyphenolic, antioxidant enriched fruit extracts from Diospyros Kaki (Persimmon), Manilkara Zapota (Cheeku) and Grewia Asiatica (phalsa) were evaluated for their anti-oxidant activities using DPPH free radical scavenging assay. Total phenolic and total flavonoid contents were estimated quantitatively. HPLC analysis was carried out for qualitative and quantitative analysis of polyphenols present in these extract samples. Finally, after phytochemical screening these fruit extract (10%) were loaded into topical emulgel formulations. The formulated emulgels (including active test formulations and respective control) were subjected to various stress storage conditions i.e. 8°C, 25°C, 40°C and 40°C+75%RH for a time period of 12 weeks, for evaluation of their physicochemical stability in terms of any change in colour, odour, consistency, pH, electrical conductivity, liquefaction, phase separation, and rheological attributes. Non-invasive In-vivo evaluation was performed using biophysical methods to evaluate the effects of active test formulations on various skin parameters such as melanin levels, erythema levels, moisture contents, sebum contents and facial skin elasticity. Surface evaluation of living skin was performed to estimate any variation in skin microreleif parameters such as scaliness, smoothness & wrinkles after treatment with active test formulation and respective control for a period of 90-days. Similarly, mean percent changes in porphyrin area, porphyrin count, porphyrin average intensity, number and area of fine and large facial skin pores was observed to elucidate and compare the effects of treatment with active test formulation to that of control. Sun protection factor was determined for formulated test and control formulations and their physical remanance on the skin was studied. Whole experimental work was carried out using single blinded, split face study design in three groups (Group-1, Group-2 and Group-3) with 13 male volunteers in each group. Results were analysed for their statistical significance using two way ANOVA and paired sample t-test. Antioxidant activities of Persimmon, Cheeku and Phalsa fruit extract were 79% ± 2.54, 82% ± 1.84 and 86% ± 1.03 respectively. Active test formulation loaded with Persimmon, Cheeku and Phalsa fruit extract and control exhibited optimum pH, electrical conductivity, and rheological attributes with good physical stability for a period of 3-months at accelerated stability storage conditions. The active test formulation EG-1, EG-2 and EG-3 produced significant (P≤0.05) while control exhibited insignificant (P>0.05) effects on melanin, erythema, sebum, elasticity, porphyrin area (%), porphyrin count and porphyrin average intensity, area and number of fine and large pores with respect to time. In case of moisture contents, active test formulations and control produced significant effects (P≤0.05), however the effect produced by active test formulation were much higher in magnitude. The active test formulation showed significant effects on skin scaliness, skin smoothness, and skin wrinkles while control produced insignificant effects with respect to time. Fruit extracts and active test formulations were shown to possess sufficient sunscreen activity and physical remanance on the skin as revealed by in-vitro spectrophotometric method and in-vivo biophysical technique. Moreover, the formulated active test emulgels were capable to quench both UVA and UVB radiations. Based on the findings of recent study it can be concluded that emulgels can be a stable, safe & cost-effective carrier system for transdermal delivery of antioxidant enriched extracts from Persimmon, Cheeku and Phalsa fruits. Moreover, formulated emulgels produced significant effects on various studied facial skin parameters by using non-invasive in-vivo biophysical techniques. Hence, it can safely be stated that these fruits can be used alone or in combination with other cosmetic ingredients to unveil their maximum beneficial potential for mankind.
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یار میرا منہ زور

یار میرا منہ زور
دتا مینوں کھور
زلف سیاہ جئی ناگن
موراں ورگی ٹور
گئی جوانی لُٹی
لے گئے کئی چور
جھوریاں ماریا آ
دکھ نہیں کوئی ہور
سولی عاشق لئی
نین سیاہ بلور
کھورے ہڈی ِمکھ
نکی جئی ہِک جھور
ڈنگے ہِک محبت
گل نہیں کوئی ہور

تحقیق و تدوین میں مخطوطہ شناسی کی اہمیت

Manuscript is a handwritten book. The writer of such book is known as "khattat". In the field of research editing and Makhtoota hold primary position. No research is possible without it. Editing is the farther goal than research. Therefore, being an expert in the manuscript is an obligatory factor for editing Manuscripts are the richest treasure of the human civilization, and their great contribution. They are the chronological records of past memories, incidents and circumstances. They need great expertise to understand these. A person who is not capable expert of manuscripts, he can’t carry out the research or editing task effectively.

Influence of Zinc on Maize and Wheat in Combination With Organic Amendments under Calcareous Soil

Organic amendment due to their variable mineralization, immobilization and adsorption capabilities need different management strategies and integration with chemical fertilizers for maximum benefits. in this regard a series of laboratory and field experiments were conducted to evaluate the role of Farmyard manure (FYM), poultry manure (PM) and biochar (BC) in Zn availability under diverse calcareous soils of Peshawar (16.6 % CaCO3) and Charsadda (6.5 % CaCO3). These studies included Zn adsorption as influenced by these amendments in Peshawar soil [Piedmont alluvium, silty clay loam, Ustochrept] followed by incubation experiment for evaluating their role in improving soil [Zn]. Finally, the effect of amendments and applied Zn was studied in field conditions in both soils for two seasons. The first season crop was maize to which these amendments and Zn levels were applied whereas the wheat was used for their residual effect. The adsorption of Zn was assumed to be the difference between initially applied Zn (IZnA) and equilibrium Zn concentration (EZnC) in soil solution after 20 h continuous shaking. The soils amended with FYM, PM or BC each at 10 g kg-1 exhibited higher Zn adsorption (x/m) at higher IAZn (40 mg to 360 mg Zn L-1) but at lower IZnA < 20 mg Zn L-1 the reverse was observed. The data in non-treated soils were fit to both Langmuir and Freundlich models but in treated soils these models were fit only when the data for initial IAZn were disregarded. The higher maximum adsorption potential b (in case of Langmuir) and K (in case of Freundlich) values as well as maximum buffering capacity (MBC) in amended soil reveals the role of organic amendments in maintaining Zn in soil solution and availability to plants. The adsorption maximum as indicating by b and K was higher for BC followed by FYM but MCB and 1/n values that reflect the buffering capacity were higher for FYM than BC or PM. The lower adsorption at IZnA < 20 mg Zn L-1 but higher at > 20 mg Zn L-1 for amended soil suggests its contrasting effect at different IZnA levels. This reveals that organic amendments will increase the Zn nutrition in low concentrated Zn soils but on the other side can help in reducing its availability in highly contaminated soils. The incubation study was conducted to evaluate the comparative role of FYM, PM and BC in maintaining soil Zn of high limed, Peshawar and low limed, Charsadda soils during 84 days of incubation. The soils were first amended with the given organic xvi amendments at 10 g kg-1 and then supplied with Zn levels at 0, 1.5 and 3.0 mg kg-1 soil. The treatments were arranged in completely randomized design with 4 (amendments type) x 3 (Zn levels) x 2 (soil types) factorial arrangements replicated three times. Significantly higher AB-DPA extractable [Zn] levels were observed by PM followed by FYM in the initial intervals but by BC after 56 d of incubation suggesting its increasing role in soil fertility in long run. The combine application of amendments with Zn levels, further improved the recovered AB-DTPA extractable Zn at given incubation advocating the potential advantage of integrated use of organic and inorganic amendments in the given soils. Among amendments, the PM+Zn exhibited higher [Zn] in both soils followed by FYM+Zn. However, the effect of BC+Zn which remained lower to FYM+Zn in both soils at earlier incubation intervals superseded it at later stage affirming the potential role of BC in the long run. This incubation study corroborates the field trials where the BC+Zn was better in wheat whereas the FYM+Zn in maize. In the field experiments, Zn at 0, 3 and 6 kg ha-1 were applied alone or in combination with FYM, PM or BC each at 20 t ha-1 in both sites. After maize, the same plots were sown with wheat crop for residual effect. Growth and yields of first season maize and succeeding wheat crop increased with increase in Zn levels and amendment application but the response was comparatively higher in high limed Peshawar than Charsadda soil that may be attributed to its initially lower fertility and poor soil conditions. Among amendment, PM showed better performance in increasing crop growth and yields in both locations and crops followed by FYM in maize but in succeeding wheat the biochar became more effective withstanding statistically at par to both PM and FYM. This increasing promising effect of BC with time reveals its lower but long lasting effects in crop production. The application of Zn with amendments also significantly increased the grain Zn and postharvest soil [Zn] in both locations and crops. PM was more effective but with either 3.0 or 6.0 kg Zn ha-1 it induced similar increases over control in contrast to FYM and BC where grain Zn increased with increase in applied Zn levels. This could be attributed to high Zn contents in PM that need less applied Zn. Results concluded that application of PM with 3.0 kg Zn kg-1 and FYM and BC with 6.0 kg Zn ha-1 would be the optimum levels for first season crops but in the succeeding crops as residual effects higher Zn application would be required irrespective of amendment type in the given soil and climatic conditions. Furthermore, the application of BC is recommended for long lasting results but its initial higher cost may hinder its application at larger scale Key words: Organic amendments, zinc, plant growth, Zn adsorption, biochar, isotherm model, , calcareous soil