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Formulation, Development and Cosmeceutical Evaluation of Creams on Human Skin Containing Natural Phenolics

Thesis Info

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Author

Bushra Arshad

Program

PhD

Institute

The Islamia University of Bahawalpur

City

Bahawalpur

Province

Punjab

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2770/1/Complete%20thesis.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726235998

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Natural antioxidants acquired from natural plants seek considerable attention to cure various ailments including acne, melasma, itching, inflammation, dry skin, atopic dermatitis and aging because synthetic agents have numerous side effects. The current study was aimed to fabricate and stabilize the novel carrier system in the form of topical water-in-oil emulsions (creams); encapsulated with natural extracts of Ananas comosus (F1), Amomum subulatum(F2) andSyzygium cumini (F3) having comparable antioxidant activities, their in-vitrocharacterization and in-vivo evaluation on human volunteers.The extracts of Ananas comosus, Amomum subulatum and Syzygium cuminiwere effectively prepared and investigated for their biological activities. The antioxidant activities of all the extracts were analyzed using1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical method while ascorbic acid was used as a standard antioxidant. The total phenolic contents were determined in all fruit extracts using Folin-Ciocalteu’s (FC) method.Antibacterial activities of all extracts were estimated by measuring zones of inhibition against two species of gram positive bacteria and three species of gram negative bacteria using agar well diffusion method. Stable water-in-oilemulsions (creams); F1, F2, F3were formulated using different concentrations of paraffin oil, water and non-ionic emulsifying agent i.e. polysiloxane polyalkyl polyether copolymer (Abil ® EM 90) versus respective bases B1, B2, B3 (without fruit extracts).In-vitro characterization of the formed emulsions F1, F2, F3 and their respective bases B1, B2, B3 were based on to assess the changes on physical stability parameters (color, liquefaction, centrifugation,viscosity and rheological parameters)when stored at different temperatures i.e. 8°C, 25°C, 40°C, 40°C ± 75% RH and 50°C and time intervals for a period of 90 days as per stability study guidelines. In-vivo evaluation was based on to scrutinize the effects of formulationsF1, F2, F3 and respective bases B1, B2, B3 on various skin parameters including skin irritancy, melasma, sebum contents, moisture contents, transepidermal water loss (TEWL) and skin elasticity on human male volunteers for a study period of 12 weeks using photometric device in a draught free room with modulated conditions of temperature (22-25 0 C) and humidity (55-60%). The study was single blinded, controlled, split face with 3-groups (F1, F2, F3) containing 11 volunteers each. Statistical tools of ANOVA test and paired sample t-test were used to evaluate the changes produced. The results of antioxidant activities of formulations F1, F2, F3 were 92.0%, 89.0% and 81.0% respectively. Selected formulations F1, F2, F3 as well as respective bases B1, B2,xxiii B3remained stable at all storage conditionswith respect to color, liquefaction, centrifugation,viscosity and rheology as desired for 12 weeksin vitro study period.All the samples manifested shear thinning behavior with increasing shear rate indicating pseudoplastic flow of the formed emulsions. It was evident from the results of in-vivo studies that no primary skin irritancy was observed with patch test. Besides that, statistical interpretation indicatedthat treatment with formulations F1, F2, F3 was superior as compare to respective bases B1, B2, B3 because it significantly (p≤0.05) reduced the skin irritancy,melasma, sebum and TEWL contents throughout the study period while significantly (p≤0.05) improves the epidermal hydration level and skin elasticity at the end of study period compares to baseline value. Conclusively, formulations F1, F2 and F3 encapsulated with natural antioxidants exhibit better physicochemical stability at all storage conditions and was well tolerated by all volunteers and suitable to treat contact dermatitis, greasy skin, acne, seborrheic dermatitis and augmenting beauty and attraction by depigmentation of human skin and represent a propitious improvement in skin barrier function and used as a functional moisturizing and anti-aging ingredient in topical skincare products. So, in future there is need to clinically evaluate these formulations in patients with compromised skin functions i.e. contact dermatitis, melasma, acne vulgaris and atopic dermatitis in order to explore the actual potential of these fruits.
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فرخندہ رضوی کی تخلیقات

فرخندہ رضوی کی تخلیقات
سنو خموشی کی داستان
فرخندہ رضوی کا پہلا شعری مجموعہ ہے۔ جو جنوری 2002 میں شائع ہوا۔ یہ شعری مجموعہ آزاد اور نثری نظموں پر مشتمل ہے۔ جسے آئیڈیل پبلی کیشنز اردو بازار کراچی پاکستان نے شائع کیااور جس کی کمپوزنگ کا فریضہ فیضان صابری کمپوزنگ سنٹر نے سرانجام دیا۔اس وقت کے مطابق اس شعری مجموعے کی پاکستانی قیمت 225 روپے اور برون ملک 8 امریکی ڈالرز تھی۔
اس کا انتساب فرخندہ رضوی نے اپنی جان سے پیاری دوست ثمینہ کے نام کیا۔ یہ شعری مجموعہ ایک سو آزاد اور نثری نظموں پر مشتمل ہے۔ اس شعری کتابچہ میں انتساب کے اگلے صفحے پر اپنے خیالات کا اظہار فرخندہ رضوی نے اس کے بارے میں یوں کیا ہے۔ ’’مجھ میں ایسا کچھ نہیں کہ مجھ سے ملا جائے اتنا کافی ہے کہ تحریروں کو ملاقات کا ذریعہ بنائیں‘‘
بس جذبوں کی سچائی لفظوں میں سمیٹتی رہی ہوں۔یہ راہیں یہ سفر پرانا ہے۔اپنی کاوشوں کو تحریری صورت میں مختلف میگزین میں بکھراتی رہی ہوں۔محبت کو جنون کا نام دیا ہے میں نے۔شعری ذوق کسی کا ورثہ نہیں۔ جو چاہے دل کی دھڑکنوں سے نکلی ہر سانس کو لفظوں میں بیان کر سکتا ہے۔
مجھ جیسی کم تعلیم یافتہ ہستی کا آسان لفظوں میں یہ سمجھا دینا کہ لہریں جوش سے مچلتی ہیں تو ساحل اور قریب چلا آتا ہے۔ اپنی تمام تحریریں بہت محبت سے پیار سے پڑھنے والوں کے نام۔ (بہت پیار کرنے والے میرے جیون ساتھی کی اجازت سے)( فرخندہ رضوی)
"سنو خموشی کی داستان" کے حوالے سے عرض ناشر میں سلیم احمد یوں رقم طراز ہیں :
" فرخندہ رضوی جو آج کے دور کی شاعرہ ہیں، ان کا کلام" سنو خموشی کی داستان" آزاد شاعری میں ایک نیا منفرد کلام ہے جو قارئین کو ضرور ضرور پسند آئے گا"(23)

Nodular hidradenoma: A worrisome mass lesion

Nodular hidradenoma is a benign neoplastic lesion of the sweat gland which is rare in its occurrence. It commonly occurs in the upper trunk and extremities. Complete excision is the only surgical treatment and avoids recurrence. We herein report a case of nodular hidradenoma that presented as a right shoulder lump in a middle-aged woman. Clinical and radiological struggles were futile to establish a definitive diagnosis of this lesion. However, histopathology of the excised tumor unveiled the diagnosis of nodular hidradenoma.

Gene Mapping in Families With Inherited Epilepsy Syndromes

Epilepsy is a mysterious problem of nervous system. It has been reported in earliest times of human history. The epilepsy syndromes are hard to define; they are so heterogeneous phenotypically that diagnosis becomes a problem for clinicians. Two or more unprovoked seizures are criteria to diagnose epilepsy in any patient. Along with environmental factors, genetics plays a significant role in causation of epilepsy. Inherited epilepsy when reported with other anomalies is called an epilepsy syndrome. In inherited epilepsy syndromes, efforts have been made to identify the disease-causing gene mutations in humans. In this study, seven families were identified with affected individuals showing neurological symptoms including epilepsy. They include two families (Families A & B) with Lafora Disease (LD), One family (Family C) with Hereditary Spastic Paraplegia 26 (HSP26), One family (Family D) with Bainbridge-Ropers Syndrome (BRS), One family (Family E) with Chorea-Acanthocytosis (ChAC) and two families (Families F & G) with epilepsy and neurological signs. By using different next generation sequencing platforms, causative mutations were identified in four families. Lafora epilepsy disease (LD; MIM 254780) is myoclonic epilepsy having autosomal recessive inheritance. The onset is from late childhood to early adolescent. EPM2A, NHLRC1 and PRDM8 genes have been identified previously to be mutated in affected individuals with LD. The families, A and B are unrelated consanguineous families belong to Pakistan showing features of LD. Affected members in both families have, generalized tonic clonic seizures, cognitive decline, and intellectual disability with ataxia. Lafora Disease was diagnosed by both histo-pathological analysis of the skin biopsy and electroencephalogram. Illumina TruSight One Abstract x Sequencing Panel covering 4813 OMIM genes was done for family A. We identified a homozygous mutation c.T94G; p.W32G of EPM2A gene which was found co-segregated in this family through Sanger sequencing. Bi-directional sequencing was done for both EPM2A and NHLRC1 genes in family B but no mutation was identified. Hereditary Spastic Paraplegias (HSPs) is a group of heterogeneous disorders characterized with progressive spasticity and weakness of the lower limbs sometimes combined with additional neurological like ataxia, epilepsy, neurodegenerative and neurodevelopmental disorders. HSPs have more than seventy types. Family C belongs to Kingdom of Saudi Arabia (KSA) having consanguineous union. The affected individuals have episodic ataxia with myokemia and microcephaly. The index case presented with episodic ataxia with myokemia, proximal muscle weakness, preserved DTR and intact cognition. Genetic analysis identified a novel missense homozygous mutation in B4GALNT1, c.C1358G:p.P453R by using whole exome sequencing of the two affected individuals in the family (HSP26; MIM 609195). The mutation was co-segregated in the family by bi-directional sequencing. Bainbridge-Ropers syndrome (BRS; MIM 615485) is a rare genetically inherited disorder with severe developmental delay, feeding problems, short stature, epileptic seizures in some patients, characteristic facial appearance. Family D is non-consanguineous family belonging to Republic of South Korea. The index case is affected male who was presented to clinic with global developmental delay, myoclonic seizures and dysmorphic features. His myoclonic seizures made it suspicious case of Lafora body’s disease. Whole exome sequencing was done, and data analysis found a de-novo substitution deletion mutation in ASXL3 gene: c.1314_1316delinsA:p.S439Rfs*7/+ that was confirmed by bi-directional Sanger sequencing and was absent in his parents. Abstract xi Chorea-Acanthocytosis (MIM#200150) is a rare progressive disorder associated with neurodegeneration. The mutations in VPS13A gene are reported to cause the disease. The clinical features are loss of cognitive and locomotor functions with severe tics. In Family E, a three generation pedigree from Pakistan, clinical diagnosis of the affected members revealed phenotypes of episodes of seizures, tics, hyperactive behavior, tongue and lip bites with psychiatric problems. Through whole genome Copy Number Variation (CNV) data analysis, a ~1.2 Kb deletion was identified in VPS13A gene already implicated in Chorea-Acanthocytosis, and found co-segregated with disease phenotype in this family through Sanger sequencing. Two other families with multiple affected individuals (Families F and G), showed multiple neurological deficits including epilepsy. Whole exome sequencing and extensive data analysis was unable to find segregation of potential selected variants in respective families. These results show the limitations of WES for identification of disease gene causing mutations in complex epilepsy syndromes. In future, whole genome sequencing would be used to resolve these cases. At present, bioinformatics tools are playing critical roles in prediction and analysis of the disease associated variants. We selected 32 epilepsy associated risk loci (available online) from six Genome Wide Association Studies (GWAS) to predict proxy Single Nucleotide Polymorphisms (SNPs), from SNAP tool based on linkage disequilibrium. These results were then used as input data for RegulomeDB; a software for understanding of regulatory elements in human genome to predict their potential functional roles in epilepsy. Only 10 SNPs returned with significant scores, indicating the regulatory function. Structural insights of the human B4GALNT1 protein were predicted by In-silico studies. One signal peptide and one trans membrane domain predicted in human wild type B4GALNT1 protein with aliphatic Abstract xii index of 92.76 and theoretical (iso-electric point) pI of 8.93. The protein showed interaction with different proteins including ST8SIA5, SLC33A1 and GLB1. Impact of all reported missense mutations in B4GALNT1 (in cHSP26 phenotype) has shown the decrease in stability of protein. Next generation sequencing along with bioinformatics analysis is growing field of clinical genetics to precisely diagnose the heterogenous inherited disorders like epilepsy syndromes. The present study will help genetic counseling of the families. It also has impact on establishment of clinical genetics studies in families with complex epilepsy syndromes and to understand molecular mechanisms involved in these disorders.