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Functional Analysis of Serotonergic G-Protein Coupled 5-Ht1a and 5-Ht2a Receptors for Determination of Serotonin Effects During Skin Healing

Thesis Info

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Author

Sadiq, Alia

Program

PhD

Institute

National University of Sciences & Technology

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Virology & Immunology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/10134/1/Alia%20Sadiq_Virology%20%26%20Immunology_2018_NUST_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726244042

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Skin wound impairment is an emerging global public healthcare challenge due to high mortality rate. It needs a comprehensive therapeutic approach for faster healing by regulating respective cellular responses and signalling cascades, which involved in healing mechanism. Post-burn skin injuries raises serotonin content at skin tissue level. This raised level is an indication about its potential role in skin healing process. Serotonin (5-HT) is important biochemical mediator and neurotransmitter that induce cellular effects through membrane bound serotonergic G-protein coupled receptor interactions. Therefore, this research dissertation was designed to investigate the role of serotonin via serotonin receptor 1A and 2A interactions in therapeutic perspective for skin wound healing by employing thermal and excisional wound injury models. Primarily, effect of serotonin has been analyzed on human neonatal keratinocytes and fibroblasts, through in vitro assays. In vitro experimental results demonstrated that serotonin treatment enhanced the cell survival (viability), proliferation and cell migration in keratinocytes and fibroblasts, whereas decreased the apoptosis. In vivo investigations based upon thermal and excisional injury model, analyzed wound healing process through immunohistochemical staining for various markers for epidermis and dermis. Results revealed that serotonin (endogenous) improved the wound healing process in control as compared to fluoxetine and ketanserin groups that exhibiting poor re-epithelisation, big wound area, delayed differentiation phase, consequently exhibited poor wound healing outcomes. Further investigations on 5-HTR1A knockout model showed impaired healing mainly due to disturbed inflammatory phase because of 5-HTR1A deficiency. Additionally, topical treatment of 5-HT1A receptor agonist in excisional injury significantly decrease the wound size, wound cellularity and ix improved the healing process in another excisional wound injury mice model. Furthermore, blocking serotonin receptor 5-HT2A interaction also exerted deleterious effects on wound healing, highlighting the importance of serotonin via 5-HT2A receptor during skin healing. Therefore, current research study revealed that serotonin acts as growth promoter by enhancing cell proliferation in skin fibroblasts and keratinocytes and imparted positive effects on skin healing by inducing cellular stimulation via 5-HT1A and 5-HT2A receptors. These results signify the potential of serotonin as an important therapeutic candidate to improve post-thermal and post-excisional skin wound healing.
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مولانا احمد سعید

مولانا احمد سعید
افسوس ہے کہ مولانا احمد سعید صاحب صدر جمعیۃ علمائے ہند نے ۴؍ دسمبر کو انتقال فرمایا، مولانا مرحوم ان علمائے دین میں تھے جن کی پوری زندگی ملک و ملت کی خدمت میں گزری۔ وہ جنگ آزادی کے نامور مجاہد تھے اور اس راہ میں بارہا قیدوبند کی مصیبتیں جھیلیں، مولانا کفایت اﷲ صاحب اور مولانا حسین احمد صاحب مرحوم کے بعد اس طبقہ کے علماء میں ایک مولانا احمد سعید ہی کی ذات رہ گئی تھی۔ وہ بھی ہم سے جدا ہوگئے۔
افسوس کز قبیلۂ مجنون کسے نماند
دینی، ملی اور سیاسی خدمات کے ساتھ مولانا بڑے خوش بیان خطیب تھے، دلی کی ٹکسالی زبان بولتے تھے، باتیں کرتے تو منہ سے پھول جھڑتے تھے، شرافت اور وضعداری میں بھی دلی کی پرانی تہذیب کا نمونہ تھے، طبعاً بڑے زندہ دل اور خوش مذاق تھے جس محفل میں بیٹھتے تھے اپنی باتوں سے پوری محفل کو مائل کرلیتے تھے۔ عرصہ ہوا ایک سیاسی مقدمہ کے سلسلہ میں ان کو کچھ دنوں اعظم گڑھ میں رہنا پڑا تھا۔ دارالمصنفین میں قیام تھا۔ اعظم گڑھ ہی کی عدالت سے ان کو سزا ہوئی اور یہیں کے جیل میں قید ہوئے، اس لیے مولانا کو قریب سے دیکھنے کا موقع ملا، اسی زمانہ سے ان کے حسن اخلاق اور پرلطف صحبت کا جو نقش قائم ہوا تھا وہ اب تک باقی ہے۔ مولانا اپنے دور کی آخری یادگار تھے۔ اس لیے ان کی وفات سے ایک دور کا خاتمہ ہوگیا، اﷲ تعالیٰ اس خادم دین و ملت کو اپنی بے پایاں رحمت و مغفرت سے سرفراز فرمائے۔
(شاہ معین الدین ندوی، دسمبر ۱۹۵۹ء)

 

Sir Syed Ahmad Khan and his Historical Writings (Notes)

Sir Syed Ahmad Khan was interested in history and history-writing. His areas of interest were Islamic and South Asian history. In this essay, we examine certain relevant facets of this interest and its manifestation. There are a vast number of studies on the life, achievements and goals of Sir Syed Ahmad Khan [1817-1898]. His essays have been collected from his journalism in 18 volumes by Muhammad Ismail Panipati1 and his other writings, lectures and letters have also received editorial attention. A useful tool in this regard is the compilation titled Kitabiyat-i-Sir Syed made by Ziauddin Lahori.2 As this paper focuses on Sir Syed’s historical writings, we may mention here a recent doctoral thesis of Syeda Jabin Zehra on this very topic.3 As a matter of fact, any and all of Sir Syed’s writings be they on education or religion now come under the ambit of history but our narrower focus will be on those writings that deal with history as history alone though they may also admit of other classifications.

Al Hidaya Wa Al Dalala Tashkeelan Wa Mafhooman Fi Doil Quran

The Almight Allah has revealed the Holy Quran for the guidance of humanity to spread the light of Hidaya and to eradicate the darkness of ignorance. It is also a wonderful source of rhetoric and eloquence. I have selected the following title for my PhD dissertation “Alhidya and Al Dhalala, Rhetorically and Morphologically in the Light of the Holy Quran”. I have divided my topic into five chapters and to several sub chapters. First Chapter: Meanings of Al-Hidaya and its synonyms; consists of synonyms, literal and terminological meanings of Al-Hidaya, its kinds and rules, and the characteristics of the guided people in the light of the Holy Quran. Second Chapter: This chapter includes the concept of al-Dhalala, its synonyms, its literal and terminological meanings, types and regulations, and the symptoms of the astrayed in the light of the teachings of the Holy Quran. Third Chapter: It includes thematic analysis of the verses mentioning the concepts of Al-Hadiya and Al-Dhalala. Fourth Chapter: It consists of terminological analysis of the verses regarding Al-Hidayah and Al-Dalala and their derivative mentioned in the Holy Quran. Fifth Chapter: In this chapter, I have discussed the rhetorical analysis of the verses regarding the topic.