67. Al-Mulk/The Sovereignty
I/We begin by the Blessed Name of Allah
The Immensely Merciful to all, The Infinitely Compassionate to everyone.
67:01
a. Blessed is HE WHO holds the reins of Sovereignty over all existence in HIS Hand,
b. and HE Manifests Sovereignty over all existence.
67:02
a. The One WHO created the phenomenon of the death and the life.
b. So that HE may test you between life and death to see which one of you would be most virtuous in deeds that pleased HIM.
c. And HE is The Almighty, The Ever-Forgiving.
67:03
a. It is also HE WHO created the seven celestial realms, one above the other in full harmony with one another.
b. You will not see any imperfection in this creation of The Immensely Merciful.
c. Then turn up your sight again!
d. Can you see any discrepancy or flaw?
67:04
a. Then turn up your sight again and again!
b. Your sight will come back to you bewildered and fatigued, unable to find any discrepancy
or flaw.
67:05
a. And, indeed, WE adorned the lowest sky to the earthly life with lamps,
b. and WE made such shooting stars/meteors that would fend off every approaching satanic evil force,
c. and, additionally, WE have prepared for them the punishment of the Blazing Fire.
67:06
a. And for those who disbelieve in their Rabb- The Lord is the punishment of Hell.
b. And it is going to be an awful and woeful destination!
67:07
a. As they would be flung into it, they will hear its furor - as it boils up,
67:08
Mufti Muhammad Shafi' was a great spiritual leader, Pakistan's grand mufti and a renowned Islamic scholar as well. He served in different fields with his knowledge, research and writings. He translated and wrote the interpretation of the Holy Qur’ān, he had keen grasp on Fiqh, Hadith and other religious affairs. Thousands of fatwas had been issued by him and he built a well known Islamic university named ‘Jamia Darul uloom Karachi.’ He participated in politics as well for the national cause and struggled too much for the Islamic contribution in the constitution of the Islamic Republic of Pakistan. This research paper is about his efforts that he made for the life of the Holy Prophet Muhammad (peace be on him). He wrote several books on Seerat-e-Nabawi, he defended Khatm-e-Nubuwwat and fought against Qadyaniyyat. This report has the abstract frame on specific grounds regarding Sīrah, Sunnah and teachings of Holy Prophet. It will portray a very brief description on Mufti Shafi's services in Sīrah's aspect.
The Antifreeze protein gene (1.022Kb) from local of D.carrota cultivar T29 was amplified from four weeks old seedling leave tissue genomic DNA. The PCR amplified gene was cloned in TA-Cloning vector pCR2.1. The cloned DcAFP gene was then sequenced, the DNA BLAST homology with wild carrot AFP was 96.1%. The 999bp gene of DcAFP from T29 cultivar was translated into amino acid sequence, the translated amino acid sequence when aligned with wild carrot AFP gene of amino acid sequence. The gene has 13 amino acid variability. The Asparagine amino acid residues which play an important role as an ice interacting/ice binding sites were intact as in wild species of carrot amino acid sequence, but extra three Asparagine amino acid were noted in 332 amino acid gene sequence as in the case of DcAFP (T29). The 944bp DNA sequence that code the mature peptide of DcAFP was cloned in pET15b E.coli expression vector. The IPTG induced 36kDa protein detected from the BL21 expression host transformed with expression plasmid. The 36kDa recombinant protein inclusion refolded and purified for polyclonal antibody production. Purified IgG of DcAFP was used in western blot, protein dot blot and ELISA analysis of transgenic potato lines. The 999bp full length gene was ligated in pCAMBIA-1301 without selection marker. Plant expression construct pCAMBIA1301hph - DcAFP (T29) was transformed in Agrobacterium host strain LBA4404. Agrobacterium with pCAMBIA construct was transformed in potato nodal explant. The transgenic plant first selected by PCR, DNA dot blot and GUS reporter assay. Stable integration confirmed by southern blot analysis. The DcAFP mRNA detected by Northern blotting after cold induction of transgenic potato plants. DcAFP protein detected by protein dot blot and western blotting methodologies. The sandwich ELISA was successfully done for expressed protein estimation in transgenic potato lines. The stable potato transgenic lines were tested with ILA (Ion Leakage Assay), which results are significant when compared with the control plants. It was noted that due to DcAFP protein expression in transgenic potato lines demonstrating that transgenic plants are tolerating the frost temperature.