The plant proteinase inhibitor-II (PI-II) proteins are diverse group of proteins which have been intensively investigated due to their potential role in providing plant protection against different environmental stresses. These genes have been implicated in the regulation of various physiological functions in plants such as modulation of plant growth and development, regulation of endogenous proteases, and mediating defense responses. In the present study, PI-II gene sequences from ten different plants were computationally analyzed using bioinformatics tools. The obtained results identified highly conserved domains in all analyzed sequences. Further, phylogenetic comparisons of PI-II genes representing ten different plants suggested that the high rate of retention of gene duplication and inhibitory domain multiplication may have resulted in the expansion and functional diversification of these proteins. The induction of PI-II gene is stimulated by various physical and chemical signals like mechanical wounding and by interplay of related phytohormones. Therefore, Agrobactreium-mediated transformation was carried out in tobacco with tomato PI-II gene under the regulation of rice root germin-like protein 2 (OsRGLP2) promoter. T1 transgenic seedlings were used for expression analysis in response to wounding, abscisic acid (ABA), methyl jasmonate (MeJA) and salt stress treatments. From the results of qPCR, it was found that ABA and MeJA dependent signaling pathways are involved in stimulation of PI-II gene. The PI-II induction by ABA and MeJA indirectly indicates its defensive role against environmental biotic and abiotic stresses such as insects, pathogen, drought and salt stresses. Transgenic seedlings subjected to salt stress condition showed significant PI-II gene activity under OsRGLP2 promoter in transgenic lines. Phenotypic analysis revealed that transgenic plants had comparatively higher level of chlorophyll content, proline content and average fresh weight than wild type plants under salt stress. Moreover, relatively high GUS expression was detected in the vascular regions of leaves, stem and roots after treatment with ABA, MeJA, wounding and salt stresses. The findings of present research are useful for enlightening the role of OsRGLP2 driven PI-II gene expression to improve stress tolerance in transgenic crops.
This study discusses the strategy of strengthening religious education in Qurrota A'yun Islamic Elementary School (SDIT) in Marawola Subdistrict, Sigi Regency, with the problem is how the model of religious education learning in Qurrota A'yun Islamic Elementary School (SDIT) in Marawola Subdistrict, Sigi Regency. What is the strategy of strengthening religious education in the Integrated Islamic Elementary School (SDIT) Qurrota A’yun Marawola District Sigi Regency. This study used qualitative research methods. The research results are a model of religious education learning in SDIT Qurrota A’yun is to implement an integrated curriculum, namely between religious and general subjects, where the curriculum is designed by integrating Islamic values in learning activities. While the strategy of strengthening religious education at SDIT Qurrota A’yun was carried out in two activities namely learning and habituation. Learning activities are carried out through curricular learning and extracurricular learning.
Orientalism is an ancient tradition of Western scholarship which portrays Islam, Muslim societies and
Arabs in negative manner. Orientalist tradition was extended to Islamic legal scholarship during colonial
era. Ground breaking publication of a Hungarian orientalist Ignac Goldziher (d.1921) `Introduction to
Islamic Theology and Law' (1910) became the basis of all future writings on this subject. Joseph Schacht
(d.1969) a German orientalist carried his work in Goldziher's footsteps and published `Origins of
Mohammadan Jurisprudence' (1950) and 'An Introduction to Islamic law' (1964) which laid the
foundations of Islamic legal orientalism in academic writings of the Western world.
With the advent of orientalist tradition in USA during the Cold War era, leading American universities
established Oriental Studies, Near Eastern Languages and Cultures and Middle Eastern Studies
departments which worked closely with U.S. State department and Foreign Affairs department to
facilitate the goverhment to establish political hegemony over the Middle East and the Muslim world.
Two major assumptions put forward in the academic debate by the West on 'Origins' of Islamic law were
inauthenticity of hadith literature and influence of pre-Islamic non-Arab laws on the development of
Islamic law. This dissertation carries out critical analysis of writings of Western scholars during cold war
era through post 9/11 era to date, on these two issues.
The objective of this research is to study the shift in paradigms, trends, approaches and methodology
adopted by Western scholars of 21' century in their writings on Origins of Islamic law and to see how this
scholarship is related to European and American political hegemony over the Muslim world. Writings of
Harald Motzki, Jonathan Brown, Wael Hallaq and Patricia Crone and a few more are selected to analyze
21' century Western scholarship. Responses, rebuttals and critiques on the assumptions put forward by
Western scholars of 20th century are also part of this dissertation. A shift in trend and methodology is
observed in 2l' century Western scholarship on Islamic law but the essential paradigms for the study
remain the same thus putting forth similar results.
It is also observed that Western scholarship on Islam is strongly connected with its political hegemony
over the Muslim world. Methodologically Western scholarship has shifted from 20th century philological
zeitgeist to 21' century social science research but it remains within the orientalist problematique. Thus
Islamic law is portrayed as an essentially defective legal system and hadith literature the second primary
source of Islamic law remains inauthentic in Western scholarship. However there are scholars who are
aware of the fact that they are not to become hostage to biased paradigms and inadvertent commitments
to political agendas in order to promote academic honesty.