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Home > Fungal Extracts Against Fusarium Wilt and Isolation of Their Antifungal Compounds Using Bioassay Directed Apporach

Fungal Extracts Against Fusarium Wilt and Isolation of Their Antifungal Compounds Using Bioassay Directed Apporach

Thesis Info

Access Option

External Link

Author

Zulqarnain, ,

Program

PhD

Institute

The University of Agriculture

City

Peshawar

Province

KPK

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Agricultural Technology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12277/1/Zulqarnain%20agri%20chemistry%202019%20uni%20of%20agri%20peshwar%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726247074

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Fusarium oxysporum f.sp. lycopersici (Fol) cause Fusarium wilt, a devastating disease in tomato, affecting the crop yield and causes economic loss worldwide. Synthetic fungicides are widely used in controlling the wilt disease, however they have been questioned due to their environmental concern. Therefore, this study was designed to screen fungal species for their antifungal potential against this destructive disease. Initial dual culture assay of fungal species revealed Aspergillus niger, Penicillium EU0013 and Alternaria solani as inhibitory to the pathogen due to their higher inhibition (%) 51.54 ± 1.09, 63.85 ± 3.26 and 46.15 ± 2.18 % respectively. During culture condition optimization studies, Glucose peptone yeast extract broth (GPYB) was found suitable for higher antifungal activity and production of compounds from A. niger and Penicillium whereas A. solani showed higher antifungal activity in glucose nutrient broth (GNB). The three fungal species showed maximum zone of inhibition against the pathogen at 30 °C and at slightly acidic pH. Penicillium sp. showed maximum zone of inhibition (9.20 ± 0.14 mm) amongst the three fungal species against Fol, therefore this species was selected for in vivo pot experiments. It was observed that with the application of Penicillium extract, plants showed no symptoms of wilting even after 40th day of plantation. Bioassay guided investigation of the fungal species leaded to isolation of eight antifungal compounds. Among these compounds, two completely new from Penicillium sp. with m/z 255 and 223 [M+H]+ and one from A. solani with m/z 273 [M+H]+ were characterized. Three known compounds from A. niger with m/z 557, 571 and 665 [M+H]+ and two from Penicillium sp. with m/z 143 and 239 [M+H]+ and one from A. solani with m/z 273 [M+H]+ are reported for their antifungal activity against Fol the first time in this study. MIC value of the purified compounds showed that the compounds were active between 40 to 120 μg·mL-1. Identification and characterization of these antifungal compounds might provide lead structures for developing them as fungicide in future.
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منصف ہاشمی کی نثری نظمیں

منصف ہاشمی کی نثری نظمیں
نثر اور نظم میں کیا فرق ہے؟ نوراللغات میں ’’نثر‘‘ کی تشریح ان الفاظ میں کی گئی ہے۔ ’’وہ عبارت جو نظم نہ ہو‘‘۔ یعنی لفظ ’’نثر ‘‘ کی اپنی کوئی حقیقت نہیں ہے۔ اسے’’ نظم‘‘کے منفیانہ یا تنسیخی معانی سے ہی پہچانا جائے۔۔۔ نثر کے لغوی معنی ہیں :’’پراگندہ‘‘، ’’بکھرا ہوا‘‘ ۔اس کی صفات میں’’ خشک‘‘، ’’غیر شاعرانہ‘‘ وغیرہ الفاظ تقریباً ہر لغت میں پائے جاتے ہیں۔ نثر کو نظم سے قریب ترلانے کے لیے جو حربے استعمال کیے گئے ان میں جملوں کے آخری الفاظ کا مقفیٰ ہونا شرطِ اول تھی۔ گویا نثر پر نظم کو مسلط کرنا شرطِ اول تھی، نہ کہ نظم پر نثر کی فوقیت کو جتانا۔ درحقیقت نثر نگاری دوسرے درجے کی ادبی کاوش ہے جب کہ نظم گوئی سرِ فہرست تھی۔ بیسویں صدی کے آخری تیس چالیس برسوں میں ’’نثری نظم ‘‘ کو ادبی جریدوں میں جگہ ملنی شروع ہوئی۔ لیکن اردو نے کبھی اس بات کو تسلیم نہیں کیا کہ’’ غزل گو‘‘یا ’’نظم گو‘‘کی جگہ ’’غزل نویس‘‘یا ’’نظم نویس‘‘ بطور اصطلاح تسلیم کیا جائے۔ ’’سخن‘‘ کا مطلب ’’بات‘‘ نہیں بلکہ’’موزوں بات‘‘ تسلیم کیا گیا ۔ا س کے لوازمات میں آہنگ ، لہجہ (صوت) ،زحافات کو صفِ اول میں جگہ دی گئی۔
منصف ہاشمی کو فیس بک اور رسائل کی وساطت سے میں دو دہائیوں سے پڑھ رہا ہوں۔ ارکان اور زحافات سے معرا ہونے کے باوجود ان کا آہنگ ایسے بیانیہ پر مبنی ہے جس میں نظم کی خصوصیات موجود ہیں۔ ترصیع، تجنیس، سجع، آہنگ اور سب سے بڑھ کر امیجری، شعر یات کے تقاضوں کو پورا کرتی ہیں۔ مترنم نہ ہونے کے باوجود ان کا بیانیہ شاعرانہ غنایت کا حامل ہے۔ ان کی نظمیں مضمون نگاری کے حوالے سے خیال بندی اور معاملہ بندی کی شرائط پر بھی پورا اترتی ہیں۔
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Interpretation of the Prophetic Way of Preaching

Calling to the path of Allah has never been an easy task. It was in fact direct opposition of the prominent personalities of that time. The holy Prophet r conducted preaching (Daa’wah) in such a way that it is not monotonous for the listeners. The Prophet r did not make them sit for long and hear lengthy speeches. Preaching (Daa’wah) in today’s world is not as successful and effective mainly because it is not carried out the way as prescribed and practiced by the holy Prophet r. It is pertinent to mention here that way of preaching adopted by Prophet Muhammad r was the same as it had been the method of all Prophets before Him r. However, it can truly be said that Prophet Muhammad r developed these qualities to the extreme heights. In this article, prophetic approach of preaching was discussed in a way that may guide the preachers of modern times in efficient as well effective way. These virtues were discussed as different traits in the personality of a preacher.

Effect of Training in Microteaching on the Performance of Prospective Teachers and Their Self Efficacy in Teaching

This study was conducted in order to observe the impact of microteaching training on the performance and teaching self-efficacy of prospective teachers in their teaching practice. The objectives of the study were: (1) to observe the performance of trainee teachers after acquiring selected microteaching skills in their teaching practice; (2) to observe the performance of trainee teachers after acquiring set induction skill in their teaching practice; (3) to observe the performance of trainee teachers after acquiringpresentation skill in their teaching practice; (4) to observe the performance of trainee teachers after acquiring questioning skill in their teaching practice; (5) to observe the performance of trainee teachers after acquiring students’ re-enforcement skill in their teaching practice; (6) to observe the performance of trainee teachers without acquiring selected microteaching skills in their teaching practice; (7) to compare the performance of trainee teachers after acquiring selected microteaching skills in their teaching practice; (8) to find out the personal teaching-efficacy of the trainee teachers after acquiring selected microteaching skills in their teaching practice; (9) to find out the personal teaching-efficacy of the trainee teachers after acquiring set induction skill in their teaching practice; (10) to find out the personal teaching-efficacy of the trainee teachers after acquiring presentation skill in their teaching practice; (11) to find out the personal teaching-efficacy of the trainee teachers after acquiring questioning skill in their teaching practice; (12) to find out the personal teaching-efficacy of the trainee teachers after acquiring students’ re-enforcement skill in their teaching practice; (13) to determine the personal teaching-efficacy of the trainee teachers without acquiring selected microteaching skills in their teaching practice; (14) and to compare the personal teaching-efficacy of the trainee teachers after acquiring selected microteaching skills in their teaching practice. Fifty-three female trainee teachers of Regional Institute for Teacher Education female Abbottabad constituted the population of this study. Through matched random sampling the subjects of the study were divided into control and experimental group. Fifty subjects, 25 each in both groups formed the sample of the study. The subjects were equated in their respective groups on the basis of their pre-test observation sheet scores in their teaching practice. Pre-test post-test equivalent group design was selected for the study. Research tools included observation sheet and personal teaching efficacy scale. Already developed and validated observation sheet by (Hussain, 2001) was adopted for the present research experiment. The calculated Cronbach’s alpha co-efficient value of the questionnaire is .82. Personal teaching efficacy scale was developed and moulded in light of the standardized teacher’s sense of efficacy scale (TSES). Calculated Cronbach’s alpha co-efficient value of the questionnaire at pre-test is .83. Trainee teachers of experimental group were administered microteaching training in set induction, presentation, students’ reinforcement and questioning skills for forty days. Post-test data analysis revealed that trainee teachers of experimental group showed better performance and higher teaching self-efficacy than prospective teachers of control group in the use of four selected microteaching skills.