Search or add a thesis

Advanced Search (Beta)
Home > Gene Pyramiding for Resistance to Some Important Bacterial and Fungal Diseases in Tomato

Gene Pyramiding for Resistance to Some Important Bacterial and Fungal Diseases in Tomato

Thesis Info

Access Option

External Link

Author

Kehkashan Akbar

Program

PhD

Institute

Hazara University

City

Mansehra

Province

KPK

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Genetics

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/11372/1/Kehkashan%20akbar%20genetics%202019.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726256104

Similar


Cultivatedtomato(Solanumlycopersicum)isreportedwithlowlevelsof genetic diversity for biotic stresses. Tomato crop meets substantial damages due to several pathogens causing serious diseases. Fungicide application and cultural practices were used to manage the disease previously. The factors includinglimitedavailabilityofcommerciallyacceptableresistantcultivar and negative environmental impact of chemical application have prompted the use of identification and use of genetic resistance to complement other practices to manage the disease. The current study was performed to determine the extent of genetic variation among the selected tomato germplasm for biotic stress and incorporation of required genetic diversity into elite lines. Germplasm comprised of 46 genotypes including cultivated varieties and advanced lines developed at Hazara Agricultural Research Station, Abbottabad, Pakistan were used in this study. Initially, 46 genotypes were screened for resistant genes for fusarium wilt (I1), late blight (Ph3), bacterial wilt (Bwr-12) and tomato yellow leaf curl virus (Ty-2). Identification of resistant gene was done using SSR markers that identified 17 genotypes bearing I1 gene conferring resistance to Fusarium wilt. SCAR marker was used to screen late blight resistance gene (Ph-3). Total of 13 genotypes were identified for Ph-3 gene. 16 genotypes were identified for Bwr-12 gene conferring resistance to bacterial wilt while 12 genotypes were identified for Ty-2 gene resistant to tomato yellow leaf curl virus. Gene pyramiding for disease resistant genes was carried out by conventional crossing of cultivated variety (Roma) and advanced line (1008) . Each of the linebeartworequiredgenesandmarkerassistedselectionwasusedto identify the target segregants until the identification of stable lines with multiplegenes.RomaexhibitresistantgenesagainstI1andPh3while advance lines (1008) had resistance against Bwr-12 and Ty-2 genes. A cross was made between these two genotypes followed by series of selection and screening till F5 generation. Stable lines by pyramiding multiple disease resistant genes I1, Ph3, Bwr-12 and Ty-2 were developed. Two lines L2P2 and L2P4 with all resistant genes were developed. After gene pyramiding, agro-morphological studies were conducted using six characters comprising vine length, fruit width, fruit length, fruit weight, number of branches per plant and number of fruits per plant. Analysis of variance followed by LSD analysis depicted significant variation among the lines developed and their parents. Line with superior traits and characters was selected and identified for yield and quality enhancement programs. Data was also recorded for agro-morphological traits of all the germplasm. The data included 25 qualitative and 14 quantitative characters. Cluster analysis revealed grouping of genotypes on the basis of similarities. A total of 46 qualitative traits were divided into six clusters comprising 9, 13, 12, 7, 4 and 1 genotype in cluster I to VI respectively. Similarly 14 quantitative traits formed five clusters comprising 2, 13, 21, 3 and 7 genotypes in Group I to V respectively. Principal Component Analysis (PCA) showed positive contributionofagronomicandfruittraits.PCAextractedfivePrinciple Components having eigen values greater than 1 and accounted for 69.83% of totalvariation.PCIexplained 25.87%of totalvariation,PCIIcontributed 40.08%, PC III accounted 52.96% while 61.80% and 69.83% was contributed by PC IV and PC V respectively.Complex pattern of association was revealed by correlation studies. Path analysis showed that selection of genotypes with multiple traits and greater equatorial fruit diameter exerts positive effects in meeting the target of yield improvement. More than 50% of enough variation was investigated in the selected germplasm, which can be employed for different vital combinations as well in future breeding programs.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

مولانا مفتی کفایت اﷲ

مولانامفتی کفایت اﷲ
ٍ افسوس ہے کہ گذشتہ مہینہ ہماری دینی و ملی عمارت کا ایک بڑا ستون گرگیا، اور ۳۱؍ دسمبر کی شب کو حضرت مولانا مفتی کفایت اﷲ صاحب مرحوم نے انتقال فرمایا، مفتی صاحب مرحوم اپنے فضل و کمال، دین و تقویٰ اور فہم و فراست کے لحاظ سے طبقۂ علماء میں نہایت ممتاز اور منفرد شخصیت رکھتے تھے، دینی علوم خصوصاً فقہ و فتاویٰ میں ان کا پایہ بہت بلند تھا ان کی پوری زندگی علمِ دین کی خدمت میں گزری اور وہ نصف صدی سے زیادہ درس و افتا کی مسند پر فائز رہے، دہلی کی مشہور دینی درس گاہ مدرسۂ امینیہ کے صدر مدرس بلکہ اس کے جزو کل تھے، اور یہ مدرسہ انہی سے عبارت تھا، اس علم و تقویٰ کے ساتھ وہ ایک مجاہد کا دل اور مدبر کا دماغ رکھتے تھے، خلافت اور ترک موالات کی تحریک کے زمانہ سے لے کر ہندوستان کی آزادی تک تمام مذہبی و ملّی اور قومی و سیاسی تحریکوں میں ان کا نمایاں حصہ رہا، ایک زمانہ تک جمعیۃ العلماء کے صدر اور کانگریس کے رکن رکین رہے، اور ان دونوں کو ان کی بصیرت اور رہنمائی سے بڑا فائدہ پہنچا۔
مرحوم کا دماغ بڑا نکتہ رس اور سلجھا ہوا تھا، اور ان کی رائے نہایت متین اور صائب ہوتی تھی، پیچیدہ سے پیچیدہ گتھیوں کواپنی فراست سے سلجھادیتے تھے، اس لیے مذہبی اور ملکی و سیاسی دونوں جماعتوں میں ان کا بڑا وزن تھا، ہندوستان کی آزادی کی جدوجہد میں ان کا خاص حصہ ہے، ملکی سیاست میں وہ اخیر تک کانگریس کے ہم خیال رہے، لیکن جب سے اس پر فرقہ پرستوں کا غلبہ ہوگیا تھا، اور اس میں مسلمانوں کی کوئی حیثیت اور ان کی قربانیوں کی کوئی قدر باقی نہ رہ گئی تھی، عملاً اس سے کنارہ کش...

موقف محمود سامي البارودي ومحمد إقبال من السياسة: دراسة تحليلية وموازنة

It is obvious to everyone who has associated with Arabic and Urdu literature that both Mahmood Sami al Baroodi and Allma Muhammad Iqbal are the shining stars in the orbit of Arabic and Urdu literature, because each of them made the world busy by his literature, and tied to blow the spirit of innovation in the style of poetry and made free it from affectation and returned it to sincerity and integrity of expression, and each of them  developed his political and cultural thought by traveling to Europe. In this article we have tried to highlight the political and literary life of Iqbal and al Barood, we also tried to analyze their efforts in the field of literature and their stand on the politics

Plasma Instabilities in Linear and Quasi-Linear Regime

Kinetic Plasma instabilities are important for regulating the temperature anisotropies of electrons and ions in the solar wind. The plasma compression generates the perpendicular anisotropy, T┴ >Tll, which leads the mirror instability for high beta situation but for low beta regime the electromagnetic ion/electron cyclotron (EMIC/EMEC) instabilities. The said unstable modes including electrons are simplified and reduced quasi linear kinetic theory with assumption of bi-Maxwellian velocity distribution function. For mirror mode instability, the linear growth rate of electron mirror mode can be much higher than the proton mirror mode and electron part operates over a range of unstable wave numbers that is much broader than proton part. But in quasi linear analysis, high initial growth rate does not necessarily imply dynamical importance, the saturated magnetic field intensity associated with electrons is extremely low and that the influence on the particle temperature is minimal. The EMIC/EMEC instabilities are taken into account with parallel propagation in two dimensions. The present manuscript extends the analysis to two (or with cylindrical symmetry, three) dimensions. The analysis is further extended to include quasilinear description with the assumption of bi-Maxwellian velocity distribution function. Such an analysis lays the foundation for eventual study in which cyclotron instabilities as well as obliquely propagating unstable modes such as the mirror instability are simultaneously taken into account.