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Genetic Analysis of Cotton Genepool Revealed by Rapd - Pcr

Thesis Info

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Author

Raheel, Farah

Program

PhD

Institute

Bahauddin Zakariya University

City

Multan

Province

KPK

Country

Pakistan

Thesis Completing Year

2008

Thesis Completion Status

Completed

Subject

Botany

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/3109/1/1206S.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726269427

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The study of genetics and evaluation of Gossypium is important for improvement in the existing gene pool. Genetic variations within and among the species and cultivars of Gossypium showing different polymorphisms were studied using the Random Amplification Polymorphic DNA (RAPD) method. In order to achieve this it had first to optimize the methods by testing different conditions of DNA extraction, amplification and electrophoresis. 10-mer oligonucleotides were randomly chosen from commercial kits and employed as primers to amplify segments of the genome and the products were detected on agarose gels. DNA amplification parameters (Buffer composition, MgCl2, DNA template, primer, dNTPs, Taq DNA polymerase, sample volume, thermocycler program) were optimized to give reproducible bands. A total of 60 cotton genotypes were used to analyze the genetic diversity. Morphological characters of 13 species were studied and a dichotomous key was prepared on the bases of these characters. Twenty species of Gossypium belonging to different genomes (A-F) were analyzed by using 63 primers. The similarity coefficients ranged from 0.03 to 0.64. Maximum similarity 64% was observed between G.herbaceum and G.arboreum and lowest similarity 3% was observed between G.stocksii and G.longicalyx. The dendrogram based on similarity coefficients was constructed by using the Unweighted Pair Group Method with Arithmetic Averages (UPGMA). Twenty species of Gossypium clustered into five distinct groups, and the two species G. longicalyx and G. stocksii stands separately in the dendrogram.   Twenty cultivars of G. hirsutum were analyzed by using 75 primers. The similarity coefficients ranged from 0.53 to 0.92. Maximum similarity 92% was observed between CIM-1100 and CIM-446 and the exotic cultivars showed 53% genetic similarity with the local cultivars of G. hirsutum. The dendrogram verified that the bulk of the genotypes were clustered in five groups. To evaluate the stability of cultivars, variation within cultivars (CIM-473, CIM-448, CIM-1100, NIAB-78) was studied. Maximum similarity within the cultivars, 92- 100% was observed in NIAB-78 and minimum similarity, 80-92% was observed in CIM-1100 by using 40, 10-mer primers. Similar amplification profiles were observed in four different cultivars. Cultivars of G. hirsutum were more closely related to each other than to the wild species of Gossypium.
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اردو زبان کا آغاز و ارتقا

موضوع 10:اردو زبان کا آغاز و ارتقا
کسی زبان کے آغاز اور ارتقاء کی داستان کچھ مخصوص تہذیبی اور معاشرتی حالات سے جڑی ہوتی ہے۔ زبان اپنی ترقی یافتہ شکل اختیار کرنے سے پہلے مختلف مراحل سے گزرتی ہے۔ اسے رنگ و روپ دینے اور نکھارنے میں مختلف عوامل کار فرما ہوتے ہیں۔ اردو زبان جو آج کی چند ترقی یافتہ اور کثرت سے بولی جانے والی زبانوں میں سے ایک ہے اسے بھی معرض وجود میں آنے سے قبل مختلف مراحل سے گزرنا پڑا۔ ان مختلف مراحل اور تہذیبی اور معاشرتی عوامل کو سمجھنے کے لئے ہمیں ماضی کی طرف پلٹنا ضروری ہے۔
جیسا کہ تاریخ کے مطالعے سے پتہ چلتا ہے کہ ہندوستان کے قدیم باشندے دراوڑ تھے۔آریا قوم باہر سے آئی اور مقامی باشندوں کو پیچھے دھکیل کر ملک پر قابض ہو گئی۔آریا قوم ملک پر ایک نئی تہذیبی طاقت بن کر ابھری۔ ان کی زبان کو مرکزی حیثیت حاصل ہوئی۔ مقامی باشندوں سے میل جول کی وجہ سے آریاؤں کی زبان متاثر ہونے لگی اور بہت سے الفاظ کا تلفظ کچھ سے کچھ ہو گیا۔ آریاؤں نے اپنی زبان کو محفوظ رکھنے کے خیال سے اسے قواعدی اصولوں سے جکڑ دیا اور اپنی زبان میں صرف ٹکسالی الفاظ باقی رکھے۔مقامی اثرات اس سے پاک و صاف ہو کر ان کی زبان نے اپنا ایک معیار برقرار رکھا اور اسی معیاری زبان کو سنسکرت کا نام دیا گیا۔
اس زبان کو کافی فروغ حاصل ہوا لیکن اس کا رشتہ عوام سے کٹ گیا گیا اور ایک مخصوص دائرے تک سمٹ کر رہ گئی۔ عوام کی زبان مختلف علاقوں میں تھوڑے سے فرق کے ساتھ ایک رسم الخط میں موجود رہیں اس زبان کو پراکرت کا نام دیا گیا۔ پراکرت زبان برابر ترقی کرتی رہی اور مختلف علاقوں میں مختلف روپ اختیار کرتی رہی۔ آگے...

الإمام الطحاوي و منهجه في كتابه مشكل الآثار

Qur’an and prophetic traditions (Hadith) are the fundamental sources of Islam. Muslims believe that Qur’an is the word of God (Allah). Hadith (Prophet’s Sayings, actions and silent approvals and disapprovals for something) likewise is based on divine revelation. Qur’an affirms also this view: (God says) Your Companion (Muhammad) has neither gone astray nor has erred. Nor does He speak of (his own) desire. It is only a Revelation revealed. Al-Qur’an (53: 2-4). Allah Almighty Himself took the responsibility to guard His word (the Qur’an): (He says: ) verily, We, it is We Who have sent down the Dhikr (i.e. The Qur’an) and surely, We will got it (from corruption). (Al-Qur’an: 15: 9) on the contrary the responsibility to guard the prophetic traditions (Hadith) was put on the shoulders on the Muslim Ūmmah. The scholars of Islam (ʽulāmʼs) try their utmost to collect and save the Prophetic traditions and guard it from any alteration. To achieve this purpose, they introduced different hadith sciences to distinguished between the true and the fabricated hadith. The authentic Sunnah is contained within the vast body of Hadith literature. Different scholars have compiled the books which contain a large numbers of Ahadith, one of them is ʼimam Taḥāwi. In this article we will discuss the ʼimam Taḥāwi approach towards “Ahadith” in his book Mushkil ul Āathʼar.

Accumulation and Partitioning of Cadmium, Zinc and Copper in Cereal and Legume Crops under City Effluent Irrigation and Phosphorus Application

A survey study was conducted in urban area of Faisalabad to compare concentration of Cd, Zn and Cu in cereal and legume crops irrigated with raw effluent, tubewell and canal waters. The results showed that there were significant variation in EC, pH and concentration Cd & Cu of effluents at sampling location due to different sources of effluent and their subsequent dilution in down stream. Raw effluent has 13, 2.5 and 4.5 times higher amount of Cd, Zn and Cu than canal water, but were within permissible limits for their use as irrigation water. No significant variations in irrigation quality parameters of canal and tubewell waters at differrent locations were observed. There were elevated levels of AB-DTPA extractable Cd, Zn and Cu in effluent irrigated soils over tubewell or canal irrigated soils but all metals were with in safe limits. About 70% of the metals were deposited in upper 30 cm soil surface. Chickpea, maize and mungbean were found maximum accumulators of Cd, Zn and Cu, respectively in grains while the order of Cd in shoot was mungbean > maize > chickpea > wheat. Highest concentration of Zn and Cu was in mungbean shoots. Mungbean roots accumulated the highest amount of Cd, while wheat accumulated Zn and Cu. Effluent irrigated chickpea grain accumulated Cd above permissible limit of WHO (0.20 mg kg -1 ) with mean concentration of 0.177 mg kg -1 while maize and mungbean followed chickpea. Green house study was conducted to investigate the effect of Cd in irrigation water on behavior of Cd, Zn and Cu in soil and their uptake by legume (chickpea, mungbean) and cereal (wheat, maize) crops. Wheat yield was not affected by the application of Cd in canal water up to 5 ppm. Application of Zn and Cu together gave the highest grain yield. Concentration of Cd increased in plant parts by increasing Cd level in irrigation water. The trend of Cu was not consistent but grain Cu was generally increased in chickpea and mungbean crops at the highest level of Cd. Higher levels of Cd in irrigation water also increased grain Cu in wheat. Chickpea yield was not affected by Cd or soil treatments and grain Zn was the highest with the combined application of Zn+Cu. Grain yields of wheat, maize and chickpea were not influenced statistically by Cd in irrigation water. However, yield of mungbean was stimulated with 1 mg L -1 of Cd. Grain Cd in wheat was the lowest with 60 mg kg -1 Zn treatment but not affected in chickpea grain. In general Zn treated soil tends to decreaseCd concentration in plant showing an antagonistic effect. The Cd application in irrigation water caused redistribution of metals in grains, shoots and roots, so the total uptake was not much affected. Effect of Cu soil treatment on concentration on Zn and Cd was not consistent in all crops. Phosphorus was applied @ 0, 50 and 75 mg kg -1 soil to study its effect on Cd, Cu and Zn sorption in laboratory. Application of 75 mg kg -1 significantly increased the sorption of Cd and Zn except Cu due to the P induced sorption in effluent irrigated soils while effect of the other levels remained non-significant. The preference of sorption was found as Cd > Cu > Zn. The sorption followed the Langmuir equation. Effect of various levels of P on availability of Cd, Zn, Cu and P in effluent irrigated soils was studied where two cereals and two legumes were grown. Wheat grain yield was found maximum with half recommended dose (57 kg ha -1 ) of P 2 O 5 fertilizer treatment anog with recommended urea. While higher levels of P did not statistically affect the grain yield of chickpea. Cadmium concentration increased in wheat grain up to recommended N and P (NP 1 ) and decreased with NP 1.5 treatment. Application of P significantly decreased Cu in grain of wheat at all levels. Wheat varieties differed in yield, P concentration, shoot Cd and root Cu accumulation. Variety AS-2002 accumulated fewer metals compared with Bhakkar-2002. Chickpea grain Zn, Cd and Cu were the lowest at higher level of P (NP 1.5 ). Nitrogen application promoted Cd accumulation in chickpea while maximum uptake of Zn and P was in N application alone. Varieties differed significantly in grain yield, shoot and root Cu and P uptake. Mungbean grain Zn and shoot Cd was minimum with NP 1 treatment while grain Cu and Cd were minimum with NP 1.5 and N treatments, respectively. Grain yield was high with NP 1 and varieties differed significantly only in root Cd, grain Cu and root Zn accumulation. In maize, the highest level of P caused less accumulation of Zn, Cd and Cu in grain, and varieties differed in most parameters. Over all, nitrogen treatment (N) caused a decrease in metal accumulation in plant tissues, while P application up to agronomic level generally increased Cd, while higher levels decreased Cd and Zn in plant tissues. Increasing level of P in soil decreased AB-DTPA extractable Zn and Cu while increased Cd. Cadmium contamination of irrigation water increased Cd, while decreased Cu concentration in all plants. Application of P in effluent irrigated soils increased concentration of Cd in crops at agronomic levels, while decreased Zn and Cu.