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Home > Genetic and Dna Marker Studies for Drought Tolerance in Upland Cotton

Genetic and Dna Marker Studies for Drought Tolerance in Upland Cotton

Thesis Info

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Author

Asif Saleem, Muhammad

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2014

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2302/1/2803S.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726275646

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Drought stress is a major limiting factor in crop production. Genetic improvement is possible in cotton and other crops against drought stress by using new tools of breeding. Thirty upland cotton (Gossypium hirsutum L.) cultivars/genotypes were screened for the traits related to drought tolerance (relative water content, excised leaf water loss, cell membrane stability and biomass reduction). Two drought tolerant (B-557 and MNH-554) and two drought susceptible (FH-1000 and N-313/12) cultivars were identified and crossed to develop F1, F2 and backcross generations. Genetic analysis was conducted for relative water content, excised leaf water loss, cell membrane stability, developmental/yield and fibre quality traits (plant height, number of monopdial branches, number of sympodial branches, bolls/plant, boll weight, ginning out-turn, fibre length, fibre strength and fibre fineness). Additive, dominance as well as genetic interaction was found in the inheritance of the traits under drought stress condition. Medium to high narrow sense heritability was observed for the traits. The analysis of parental and F2 population of the cross B-557 × FH-1000 studied under osmotic stress in hydroponic culture showed that the traits, relative water content, excised leaf water loss and cell membrane stability had correlation with biomass production. Positive correlation of relative water content with cell membrane stability reveals that the genes which help plant maintain relative water content are also indirectly involved for cell membrane stability. Correlation analysis of F2 population under field drought revealed positive correlation of relative water content and cell membrane stability with plant developmental/yield and fibre quality related traits. The correlation results from hydroponic culture and field studies showed that relative water content, excised leaf water loss and cell membrane stability may be used as indicator of drought tolerance in cotton. A survey of 524 SSR and EST-SSR primers revealed a lot of DNA polymorphism between drought resistant (B-557) and drought susceptible (FH-1000) cultivar. The polymorphism was used to construct genetic linkage map using F2 population. In linkage analysis, 22 primers were mapped on chromosomes. Two QTLs for relative water content were identified on chromosome 23 and 12. One QTL for excised leaf water loss was found on chromosome 23. These QTLs may be used in molecular breeding programs to develop drought tolerant cotton varieties.
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56. Al-Waqi’ah/The Event

56. Al-Waqi’ah/The Event

I/We begin by the Blessed Name of Allah

The Immensely Merciful to all, The Infinitely Compassionate to everyone.

56:01
a. When the Inevitable Event of Doom will descend, and herald the Resurrection,

56:02
a. then there will be no belying and denying of its descent;

56:03
a. it will be degrading and humbling some humans and jinn, and exalting some others.

56:04
a. When the whole of the terrestrial world will be shaken, shaken violently in a series of massive earthquakes and volcanic eruptions,

56:05
a. and the mountains will be made to crumble, utterly crumbling,

56:06
a. so as turning it to dust, scattered in the air like puffed wool.

56:07
a. And you all will be sorted out into the following three diverse categories:

56:08
a. As for the first category:
b. the People of the right hand side – the lucky and blessed ones;
c. how lucky and blessed will be the People of the right hand side!

56:09
a. And the second category:
b. the People of the left hand side – the unlucky and wretched ones;
c. how unlucky and wretched will be the People of the left hand side!

56:10
a. And the third category:
b. those in the foremost who would have taken the lead
c. – indeed they will be the foremost in their race to seek Allah’s Countenance!

56:11
a. For those will be the ones brought near,

56:12
a. in Gardens of Perpetual Bliss - abounding in peace, tranquility, and blessings.

Efektivitas Pembelajaran Daring Mata Kuliah Fisika Di Perguruan Tinggi

Penelitian ini memiliki tujuan untuk mengetahui tingkat efektivitas pembelajaran daring mata kuliah fisika di perguruan tinggi. Penelitian ini merupakan penelitian studi literatur dengan mengambil referensi dari beberapa jurnal dan sumber pustaka dengan pemaparan secara naratif untuk melihat efektifitas pembelajaran daring di perguruan tinggi. Sebagai pendidik, dosen memanfaatkan berbagai media pembelajaran dan aplikasi digital yang diharapkan dapat terintegrasi pada pengalaman belajar mahasiswa dan tetap mengacu pada capaian pembelajaran dalam rencana pembelajaran semester (RPS). Fisika merupakan salah satu mata kuliah wajib. Pembelajaran daring pada mata kuliah fisika di perguruan tinggi memang cukup baik, namun memiliki kendala terhadap pemahaman terhadap materi fisika yang diajarkan oleh dosen di antaranya pembelajaran yang diberikan lebih dominan teoritis dan kegiatan praktikum tidak optimal dilaksanakan. Sehingga efektivitas pembelajaran daring mata kuliah fisika di perguruan tinggi dapat dikategorikan cukup efektif karena hasilnya tidak optimal.

Optical Emission Studies of Laser Induced Silicon Germanium, Tin and Lead Plasma

Laser induced breakdown spectroscopy (LIBS) is a technique which provides qualitative and quantitative information from the emission spectra of the laser produced plasma of the metallic materials in various environments. In the present work, we have studied the optical emission spectra of the laser produced silicon (Si), Germanium (Ge), Tin (Sn) and Lead (Pb) plasma and also deduced the plasma parameters and atomic parameters by this technique. We have used the fundamental 1064 nm and second harmonic 532 nm of a Q-switched pulsed Nd:YAG laser (Quantel, Brilliant-B, France) for ablation and generating the plasma. The emission spectra of the laser produced plasma were captured using a set of four spectrometers (Ocean Optics), each spectrometer was equipped with 5 micron slit width and covering the spectral region between 200 – 720 nm. In somaqe experiments, we also used another set of four spectrometers (Avantes, Holland), each spectrometer was equipped with 10 micron slit width and covering the spectral region between 250 – 875 nm. The experimentally observed line profiles of the several neutral and ionic species have been used to extract the line widths, plasma or excitation temperatures, electron number densities and atomic parameters such as branching fractions, transition probabilities and relative line strength. The Plasma temperatures were determined using four different techniques; two line ratio method, Boltzmann plot, Saha-Boltzmann plot and Marotta''s technique whereas electron densities were deduced from the Stark broadened line profiles as well as from the Boltzmann-Saha relation. From the observed line intensities of the spatially registered spectra of silicon, we have determined the plasma temperature in the range of 8500 K to 9500 K and electron density in the range of 1x1017 cm-3 to 4 x1017 cm-3 at different laser energies. The plasma temperature for germanium (Ge) has been determined in the range (9000 – 11400 K) , (9500 – 10500 K) , for Tin (Sn) and (9000 - 10500 K) for Lead (Pb) and the electron densities have been deduced in the range (0.5 – 5.0)×1017cm-3 for Ge, (0.5 – 5.0)×1017cm-3 for Tin and (3.5 – 6.5)×1016cm-3 for lead depending on the laser pulse energy to produce the plasma. The Full width at half maximum (FWHM) of a number of neutral and singly ionized silicon, germanium, tin and lead lines have been extracted by the Lorentzian fit to the experimentally observed line profiles. We have compared the experimentally measured relative line strengths for the 4p5s 3P0,1,2 → 4p2 3P0,1,2 multiplet with that of calculated in the LS-coupling scheme revealing that the intermediate coupling scheme is more appropriate for the level designations in germanium. In addition, the LIBS, technique has also been exploited to experimentally determine the branching fractions and transition probabilities for fourteen spectral lines originating from the 3p4s→3p2 transition array of silicon (Si I), fifteen lines of the 5p5d→5p2 transition array in tin and eleven lines of the 6p7s → 6p2 transition array in lead, whereas the absolute values of the transition probabilities have been calculated by combining the experimental branching fractions with the life times of the excited levels. Our new results are comparable to that reported in the literature, where available.