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Home > Genetic Divergence Estimation Through Agro-Morphological, Bio-Chemical and Molecular Markers in Sunflower Helianthus Annuus L.

Genetic Divergence Estimation Through Agro-Morphological, Bio-Chemical and Molecular Markers in Sunflower Helianthus Annuus L.

Thesis Info

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External Link

Author

Ibrar, Danish

Program

PhD

Institute

Pir Mehr Ali Shah Arid Agriculture University

City

Rawalpindi

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Plant Breeding & Genetics

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/13401/1/Danish%20Ibrar%202019%20plant%20breeding%20arid%20rwp%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726287653

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Sunflower (Helianthus annuus L.) is an important oilseed crop. In Pakistan, it is the third biggest source of edible oil. Every year Pakistan spends huge amount of its foreign reserves on import of edible oil and now Pakistan is the third largest importer of edible oilseeds in world after China and India. Our local production has decreased over the years and now our local edible oil production is only the 13% of our demand. To bridge the gap between supply and demand of edible oil in Pakistan, government has to focus on Sunflower crop. For increasing the sunflower production new and improved local sunflower hybrids should be developed. Information of Genetic diversity study is a prerequisite for designing any breeding program. In the first experiment genetic diversity among 109 sunflower lines was estimated at morphological, bio-chemical and molecular level. For morphological characterization 109 sunflower lines were evaluated during Spring-2015 and Spring-2016 at National Agricultural Research Center, Islamabad following Augmented Design. The studied genotypes showed significant amount of variation on the basis of results obtained by cluster analysis and Principal component analysis. SDS-PAGE technique was used to study the variability among the sunflower genotypes at bio-chemical level. Genetic variability at molecular level was studied using SSR markers. Morphological and molecular based characterization showed considerable amount of genetic variation among the studied sunflower genotypes, while these genotypes showed less than 3% dis-similarity through SDS-PAGE based bio-chemical genetic study. The second experiment was for the identification of heterotic groups among the studied material. Heterotic groups were identified by combing the morphological and molecular data sets as, combining different data sets has been found useful in increasing the resolution of experiment. Two major clusters one of CMS and maintainer lines and other of restorer lines were observed. Six sub-groups in each major cluster were observed and one best performing genotype from each subgroup was selected. In third experiment, 12 parents (6 CMS and 6 Restorer) selected from heterotic groups was crossed in LxT mating design during autumn, 2016 to develop crosses and study the efficacy of identified heterotic groups and gene action. 36 crosses developed along with their parents and checks were sown in Randomized Complete Block Design (RCBD) at two locations (NARC and URF) during spring-2017. Three best performing hybrids (RHP-41 x CMS-HAP-56, RHP-71 x CMS-HAP- 111, RHP-71 x CMS-HAP-12) were found to be best for seed yield and oil contents over three check hybrids and four best combining parents i.e., CMS-HAP-12, CMS-HAP-56, RHP-41 and RHP-71 were also identified. Results of our study showed that heterotic grouping among sunflower could be developed and strengthened on the basis of morphological and SSR markers to pace up the hybrid development in Sunflower.
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ہماری دعا میں اثر ہی کہاں تھا

ہماری دُعا میں اثر ہی کہاں تھا
بھلا بے وفا وہ بشر ہی کہاں تھا

میں گھر کیوں نہیں جاتا سب پوچھتے ہیں
کہوں اب میں کیا میرا گھر ہی کہاں تھا

تجھے مانگنے کے سوا دشمنِ جاں
گرا اپنا سجدے میں سر ہی کہاں تھا

جو آباد تھا کس قدر اُس کے دم سے
بغیر اُس کے ویسا نگر ہی کہاں تھا

زمانے کی حدت سے مجھ کو بچاتا
رہِ عشق میں وہ شجر ہی کہاں تھا

ہبہ مشاع اور غیر مشاع میں قبضہ

The institution of ‘Gift’ (ہبہ) is common in every religion. Every religion promo-tes this practice, as it is a tool to create sense of love and affection between the giver and the receiver. Similarly, Islam encourages its followers to perform it from time to time and spread sense of love and affection. The holy Prophet (Peace and Mercy be upon him) not only ordered the believers of Islam to exchange gifts but also, he himself was habitual of distributing things among the Muslims as gifts. Here, in this article, this exercise of the ‘Gift’ is discussed. Firstly, its lexical and terminological meanings are mentioned and supported with verses of the holy Quran and the traditions of the holy Prophet (PBUH). Furthermore, its need and importance are given consideration. Secondly, kinds of the ‘Gift’ are specified, which are ‘Gift of existing things and non-existing things’. Then the existing things are divided into ‘Devisable and non-devisable items’. In the end the concept of possession in the ‘Gift’ is stated. Along with all this opinions of the five schools of thoughts i.e. Hanafi, Maliki, Shafai, Hanbali and Shia, and their basis regarding possession of the ‘Gift’ are presented.  

Production, Purification and Characterization of Carboxymethyl Cellulase for Food Applications

Agricultural wastes/byproducts have great potential for the production of value added products with special reference to enzymes. Two indigenous carbon sources namely, wheat bran and sugar cane bagasse were used as substrates for carboxymethyl cellulase (CMCase) synthesis by Aaspergillu niger through submerged fermentation. The fungus produced highest CMCase activities at 3% level of wheat bran using 30°C and pH 5 over 96 hrs of incubation. The enzyme was purified for its further use in food processing. During the purification process, a 3.05 fold purification of enzyme was observed after gel filtration. Characterization of purified enzyme was carried out to find out the most suitable conditions for the CMCase performance; the best enzyme activity was observed at 50°C and pH 4.5. The enzyme was quite stable and retained 37% of its maximum activity at 90 ° C. The SDS-PAGE of the purified enzyme revealed its molecular weight as 47.1 kDa. For efficacy purpose, the enzyme added feeds were given to the broiler chicks in order to investigate their effects on body performance, feed efficiency and for safety concerns. The enzymatic treatment exhibited positive effects on body weight, carcass weight and chest muscle weight. Non-significant variations in the serum biochemistry and hematological parameters were observed. After safety evaluation, the enzyme was employed at various levels in bread preparation. Different treatments exhibited significant variations in rheological properties of the dough. The CMCase application in the bread making process resulted in increased volume (15.45%) up to a level of 250 IU/100g flour (T 2 ) however, excessive dose showed a decreasing trend for this parameter. The treatment T 2 produced bread with specific volume 3.99 cm 3 /g in contrast to that of T 0 i.e. 3.37 cm 3 /g. The external and internal characteristics of bread were also improved by the enzyme addition. Later, the enzyme application in citrus juice extraction also showed beneficial effects. Although the enzymatic treatment had non-significant effect on TSS, density and pH of pulp and juice however, a momentous reduction in pulp viscosity i.e. 74.79% was observed in T 6 (CMCase and pectinase 50 IU/100g each). Moreover, the treatment T 6 gave more clarified juice with 95.70% transmittance than that of control (78.00%); also resulted in 20.00% increase in the juice yield with 90.00% juice recovery. The sensory evaluation of the enzyme treated juice reflected improved taste, flavor and sweetness however; caused slight color deterioration of the resulting juice. The purified enzyme was also employed in carotenoid extraction from citrus peel and the maximum yield (8.60±0.94 mg/100g peel) was calculated in case of T 4 (250 IU of each enzyme/100g peel). The pigment showed more stability in dark as compared to light at 30°C. Furthermore, higher pigment loss was observed at 45°C than that at refrigeration temperature (4°C). It was also observed that freeze drying of the pigment enhanced its stability. It is concluded from the present investigation that wheat bran has a potential for CMCase production that can efficiently be used in bread production, juice processing and carotenoid extraction.