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Home > Genetic Diversity Studies for Whip Smut Tolerance in Sugarcane Germplasm

Genetic Diversity Studies for Whip Smut Tolerance in Sugarcane Germplasm

Thesis Info

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Author

Nazar, Zaheer Ahmad

Program

PhD

Institute

University of the Punjab

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Molecular Biology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/10484/1/Zaheer_Ahmad_Nazar_Molecular_Biology_2018_UoP_Punjab_10.04.2019.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726295803

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The performance of 103 promising sugarcane lines/varieties under high inoculum conditions for whip smut, was checked over two successive cropping years. The yield potential of these sugarcane lines was evaluated in terms of their girth, height, weight, sugar recovery and smut resistance. Eighty-four (84) sugarcane lines were found highly resistant in the first year of cultivation while only 5 sugarcane lines were found susceptible. The sugarcane lines S.2003-US-127, S.2008-FSD-19 and S.2008-AUS-190 showed peak performance with respect to sugar recovery, cane weight and height respectively. The girth of sugarcane lines S. 2011-SL-813 and S. 2011-SL 209 was found maximum. However, during the second cropping year (ratoon crop), eighty (80) sugarcane lines were found highly resistant while only four (4) sugarcane lines were found susceptible. The performance of the sugarcane lines S. 2008-US-704, SPSG-24, S. 2011-SL-642 and S. 2011-SL-209 was found highest for sugar recovery, girth, cane height, cane weight, respectively during the second cropping year. Principal component bi-plot analysis reveals over all best performance of S.2008-FSD-17, S.2003-US-127, S.2008-US-704, S.2008-FSD-19 for all yield traits while overall least performance was recorded by S.2003-US-618, SPSG-27 for all the yield traits during both years of cultivation. Higher broad sense heritability and genetic advance values revealed in this study especially for cane weight and sugar recovery indicated that these traits could be utilized for developing hybrid and synthetic varieties. Significant negative impacts of whip smut were found upon the yield traits. However, these traits were found somewhat more genetically controlled with little to moderate environmental impacts. The LSD based ranking and other statistical analysis revealed high yield potential of these sugarcane lines for being used in future breeding programs. Understanding the level of genetic variability held in the sugarcane germplasm is critical for proficient use of its gene pool. Genetic diversity based response of 103 promising ii sugarcane lines/varieties for tolerance against whip smut was the main objective of this study. Genetic similarity coefficient ranging 66-88% observed in this study, indicated narrow genetic base of these sugarcane lines/varieties which arises the need of using distantly related sugarcane lines in breeding programs. DNAMAN generated dendrogram of 103 sugarcane lines and their response against whip smut will help in designing crosses between distantly related sugarcane lines/varieties that would not only increase the genetic base of sugarcane crop but would also help in developing whip smut resistant sugarcane cultivars. Also, varying response of sugarcane lines for smut resistance in the few clusters propose that whip smut resistance is neither confined to particular sub-population nor governed by genes with broad impacts but is in fact a genuine quantitative trait. Moreover, the genotyping files developed against the 314 amplified alleles of 30 markers especially against mSSCIR-43 and mSSCIR-19 would help sugarcane breeders in molecular identification of whip smut resistant and susceptible sugarcane lines. This would ensure correct clones involved in the crosses. High PIC and RP values of the tested markers highlighted their potential of using in in genetic diversity studies. Moreover, the genotyping data of all the 103 sugarcane lines/varieties against the amplified 314 alleles and the phenotypic data of two successive cropping years, was integrated using Structure 2.3.4 and TASSEL software to find the alleles linked with smut resistance and various yield traits. These linked alleles depicted phenotypic variance (R2-values) ranging from 3.1-24.6% for smut resistance, 2.67-22.5% for sugar recovery, 1.75-12.8% for sugarcane girth, 2.81-23.46% for cane height and 2.9-14.34% of cane weight. The maximum degree of association was revealed by the allele 52-121 for cane height, 82-184 for cane girth, 51-131 for sugar recovery, 51-145 and 51-146 for cane weight and smut resistance respectively. These alleles could help in marker assisted selection of studied sugarcane lines/varieties for these traits. The little effects of various linked alleles with smut resistance and yield traits indicated that these traits and smut resistance are controlled by additive effects of multiple genes.
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2۔ دیت تغلیظ

2۔ دیت تغلیظ
دیت کو زیادہ سخت بنانا ، اس میں زیادہ شدت اختیار کرنا ۔ قتل عمد کی دیت میں شدت اختیار کرنا ۔ امام مالک ؒ کےمطابق باپ کا اپنے بیٹے کے قتل کی صورت میں دیت مغلظ ہو گی یعنی اونٹوں کی صور ت میں دیت یہ ہو گی ۔ تیس حقہ ، تیس جذعہ ، چالیس خلفہ ۔ سونے اور چاندی کی صورت میں ان اونٹوں کی قیمت میں اور عام دیت میں جو اونٹوں کی قیمت کا فرق ہے دیت مغلظہ میں شامل ہو گا ، مثلاً اگر دیت خفیفہ کے اونٹوں کی قیمت چھ سو درہم ہے اور دیت مغلظہ کے اونٹوں کی قیمت آٹھ سو درہم ہے تو یہ زائد دو سو درہم سونے یا چاندی کی صورت میں ادا کی جانے والی دیت میں شامل ہوں گے ۔ 167
امام احمدؒ کے نزدیک دیت میں تغلیظ کے تین اسباب ہیں : حرم میں قتل ، اشہر حرام میں قتل اور محرم کا قتل ۔ ان کے نزدیک تغلیظ کا طریقہ یہ ہے کہ ان ہر تین اسباب میں سے ہر سبب کی موجودگی میں ایک تہائی دیت زائد ہو گی اور اگر تینوں اسباب جمع ہو جائیں تو دو دیتیں لازم ہوں گی۔ "168
3۔ وہ زخم جہاں مکمل مماثلت کے ساتھ قصاص ممکن نہ ہو صرف دیت دی جائے گی۔ انہیں ارش بھی کہتے ہیں۔ اس کی مندرجہ ذیل تین اقسام ہیں :الف) شجہ ، ب)جائفہ اور ج)غیر جائفہ۔

أبو الأحرار محمد محمود الزبيري وخدماته الأدبية

Renowned Yemeni poet and freedom fighter Muhammad Mahmood Al-Zubairi, also known as the father of freemen, born in Sanna in 1910 in a middle class family. He was one of  the Yemeni iconic revolutionaries who opposed the Imamate. He took part in the revolution in 1962, bringing about Yemen’s transition from a monarchy to a republic. He was one of the founders of the movement of liberals and the leader of opposition against the Imam’s rule. This led to his persecution and he suffered destitution and exile outside his country, settling finally in Pakistan where he had opportunity to translate the poetry of Pakistan’s national poet, Muhammad Iqbal into Arabic. Finally, in 1962, when the revolution against the Imam erupted in Yemen, he went back to his country and became the minister of education. He fallen victim to the royalist forces in 1965 and has been regarded since as one of the Yemen’s most acclaimed martyrs. Al- zubairi published several collection of poetry. In 1978, a volume of his collected poems was published entitled Diwan al-zubairi. His work  reflects a real originality of themes, ideas and method of treatment. This article discusses the literary work  of Abu Ahrar Muhammad Mahmood Al-Zubairi.

Molecular Variation Among Bemisia Population in Cotton Growing Areas of the Punjab and Sindh

The whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is one of most destructive insect pests of agriculture and horticulture worldwide. It is a cryptic species complex, and biotypes of the complex have become serious pests in Pakistan because of their feeding and their ability to transmit cotton leaf curl virus (CLCuV). During 2007- 2008 and 2008-2009, the survey covered 40 cotton growing areas of the Punjab and Sindh provinces. Bemisia tabaci populations were sampled from cotton fields showing CLCuV infection. 100% prevalence was observed on the basis of typical begomovirus symptoms in cotton fields, both the years in Punjab and Sindh, Pakistan. The genetic diversity, biotype status of the Bemisia tabaci and its association with difference in Cotton Leaf Curl Disease incidence across both provinces were assessed by using random amplified polymorphic DNA-polymerase chain reaction and the mitochondrial cytochrome oxidase 1 (mtCO1) gene sequences. Phylogenetic analyses of mitochondrial cytochrome oxidase 1 sequences conducted by maximum-likelihood and maximum parsimony methods. The RAPD banding patterns showed considerable genetic variations among all 82 B.tabaci populations analyzed from each year. RAPD profiles generated 151 scorable amplification products, of which 77% were polymorphic in 2007 -2008 populations however 152 scorable amplification products were produced, of which 79% were polymorphic during 2008-2009 analysis. The study showed that the primer OPA-04 gave the unique and unambiguous DNA profiles that clearly distinguished biotype B from indigenous non B individuals. The primers gave polymorphic products of 150bp and 450bp with B biotype whereas 490bp and 1250bp with non B biotype. RAPD data clustered the B. tabaci samples on the basis of B and non B biotypes, regardless of the geographical origin of the populations. In both years analysis, 78 out of 80 Pakistani samples excluding the control populations fell in the non B cluster. The sub grouping between B.tabaci is diverse as Punjab populations set together with both Punjab and Sindh populations in this cluster. The B biotype populations formed an independent cluster contained B biotype reference population with two populations from Sindh. This shows that the presence of B biotype now termed Middle East Asia Minor 1 (MEAM 1) was restricted to a few locations of Sindh. This RAPD analysis showed that 96% of total B.tabaci samples studied are non B biotype (now termed Asia II 1), observed in cotton growing areas of Punjab and Sindh, Pakistan. These non B biotype B. tabaci are more diverse and indigenous to B biotype. It is estimated that genetic variations among non B populations increased by 2% over a year. Phylogenetic analyses of mtCO1 gene sequences congregated Pakistani B.tabaci with two genetic groups; Asia II 1(H, M, NA, non B biotypes) and MEAM 1 (B, B2 biotypes) in the year 2007-2008. However, the 2008-2009 samples fell into three distinctive clusters namely, Asia II 1, Asia II 5 and MEAM1. This phylogenetic inference revealed that the vast majority of the B.tabaci were Asia II 1 (non B biotype) and appeared well established in cotton growing regions of Punjab and Sindh province. This Asia II 1 cluster separated into two subgroups; subgroup A and subgroup B. The subgroup A, showed Pakistani populations shared close relationship with the Chinese, Indian and previously described Pakistani B.tabaci whereas the subgroup B clustered, Pakistani Bemisia populations with Nepal and Bangladesh populations. The high nucleotide identities (upto 99%) were observed in each group. MEAM1 was not detected in Punjab cotton growing areas by RAPD and phylogenetic analysis however identified from few locations of Sindh. Two populations from Pakpatan, Punjab and one from Khairpur, Sindh grouped with Asia II 5 (G biotype) with a high bootstrap support at 99%. The Asia II 5 is a new biotype observed in Pakistan and its presence is already reported in India and Bangladesh. This study is the first evidence of Asia II 5 presence in Punjab and Sindh, hence, the correlation between Asia II 5 and Cotton leaf curl disease (CLCuD) is yet unclear. The prevalence of Asia II 1 was sustained in these two year analysis of B.tabaci population from cotton. It seems that the distribution of this genetic group influence the distribution of CLCuD and plays a vital role in the spread of CLCuV all over Punjab and Sindh provinces, Pakistan. The phylogenetic study significantly supported the outcomes of RAPD analysis. The high genetic variations were observed in B.tabaci populations of cotton growing areas of Punjab and Sindh. The Asia II 1 (includes H, M, NA, non B biotypes) is prevalent in both provinces and associated with high incidence of CLCuD. The present study, gives the first indication of subsistence of Asia II 1 on cotton in Sindh and presence of Asia II 5 in Pakistan. This knowledge will contribute to the development of appropriate strategies with which to manage the disease in Pakistan.