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Genetic Evaluation of Maize Genotypes Using Physico-Chemical and Molecular Markers

Thesis Info

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Author

Khan, Zakiullah

Program

PhD

Institute

University of Poonch

City

Rawalakot

Province

AJK

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Plant Breeding & Genetics

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12421/1/Zakiullah%20Khan_Plant%20Breeding_2019_UoPoonch_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726297207

Similar


The presentc researchc projectc was conductedc in the Cerealc Cropc Researchc Institutec (CCRIc) Pircsabak, Nowshehrac (K.P) Pakistanc duringc summerc 2014. Six maizec (Zea mays L.) genotypesc werec used in the researchc experimentc viz; PSEV-3, Azamc, Sarhadcwhite, Jalal, Iqbalc and Paharic. The maizec genotypesc were crossedc in a completec 6x6 diallelc fashionc and theirc Fc1 generationc was studiedc. Meanc squaresc due to GCA and SCA were highly significant for all the traits in F1 and F2 generations with the exception of GCA values for Leaf Area (cm) and Moisturec % in F1 and F2. The combiningcability analysescalso revealedc that majorityc of the traitscwere controlledc by partialc dominancec with additivec genec action. In F1generationc, the highest SCAc effects were manifestedc by crossesc involving PSEV-3 i.e PSEV-3x Iqbal, PSEV-3xAzam, PSEV-3 x Sarhadc White PSEV-3 x Pahari Azam x Pahari and Azamc xIqbal. Cultivar PSEVc3 of the abovec genotypesc provedc to be the best generalc combinerc as mentionedc earlier havingc highestc GCA valuesc producedc best specificc crosses. In case of F2 generationc maximumc SCA were exhibitedc by Sarhad WhitecxIqbal and JalalcxIqbal were the best crossc combinationsc. The cultivar Sarhadc White having negativec GCAc affectsc for ninec charactersc, therefore it was concludedc that comparativelyc low x highc parentsc performedc well in SCAc determinationc. Heterosisc of varyingc magnitudec was foundc in F1 and F2c generationsc. Mid parentc heterosisc was morec pronouncedc than batterc parentc heterosisc. Yieldc and its componentsc showedc maximumc heterosisc while otherc traits showedc lowc to moderatec heterosisc. Onc the basisc of heritabilityc, GCA, SCcA and heterosisc the genotypesc PSEV-c3 x Sarhadc Whitec, Sarhadc Whitec x PSEV-c3, PSEV-c3 x Paharic, PaharixPSEV-3, Sarhad White x Iqbalc andc Iqbalc x Sarhadc White were foundc promisingc. These competitivec genotypesc had a greatc potentialc to becomec vigorc withc highc yield and bestc suitedc for generalc cultivationc. In biochemicalc studies, parentalc cross of maizec genotypesc in whichc prolinec contentsc mostlyc increased in Iqbalc which rangedc uptco 0.37 (μmol/g) andc decreasedc in Azam with 0.08 (μmol/gc). The highestc levelc of prolinec contentsc showsc in Azamc x Sarhad x White which ranged 0.28 (μmol/g) and the lowest ranged was exhibited 0.06 (μmol/g) in PSEV-3 Iqbal in reciprocal crosses. Directccross of maize genotypes presented highest level of prolinecconcentration which was 0.35 (μmol/g) in Iqbal x Sarhad White and the lowestcconcentration was 0.08 (μmol/g) in Jalal x Azam. In molecularcstudy the SSR markers were used to estimate the genetic diversity, polymorphiccinformation content and number of alleles. In the present study maize genotypes exhibited various levels of genetic polymorphism by SSR markers. In present research project eight genotypes shown high genetic diversity as compared to other genotypes. Which are Pahari × Sarhad White, Pahari × Azamc, Sarhad White × Jalal, Jalal × Sarhad White, Sarhad White × Azam, Sarhad × Iqbal, Jalal × PSEV-3 and Iqbal × Sarhad White. Geneticcdiversity in maizecgenotypes gives the opportunity of best results for future breeding programs.
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حوالہ جات

(1) حاتم ظہور الدین شیخ، دیوان زاده، مرتب پروفیسر عبد الحق (نئی دیلی: نیشنل مشن فارمین سکر پٹس)
اشاعت اول 2011، صفحہ 5
(2) توقیر احمد خان، پروفیسر ڈاکٹر قلمی مسودہ صفحہ 1
(3) حاتم ظہور الدین شیخ ،دیوان زاده، مرتب ،پروفیسر عبد الحق، صفحہ 5
(4) اقبال، کلیات اقبال اردو ،بانگ درا، والدہ مرحومہ کی یاد میں (لاہور ۔ اقبال اکادمی
پاکستان) اشاعت ششم 2004 ،صفحہ 266
(5) توقیر احمدخاں،پرو فیسر ،ڈاکٹر، قلمی مسورہ صفحہ 2
( 6) عبدالحق، پروفیسر، فکرِاقبال کی سرگذشت، ناشر، صائمہ حق، شفا حق ( جون پور:یو۔ پی۔رحمن منزل، بلوا گھا ٹ شام 1998ء) صفحہ 5
(7 ) عبد الحق، پروفیسر ،فکرِ اقبال کی سرگذشت، ناشر، صائمہ حق ، شفا حق، صفحہ4
(8) عبدالحق، پر وفیسر، عصری لغت (نئی دہلی ۔ اصیلا آفیسٹ پرنٹرز ) اشاعت دوم فروری 2006
صفحہ 5
(9) اقبال، کلیات اقبال فارسی، پیام مشرق، ساقی نامہ (لا ہور: شیخ غلام علی اینڈ سنز ) س ن ،صفحہ 285
(10) عبدالحق، پروفیسر، خط بنام ساجد ندیم، یکم جون 2014 ء
(11) عبدالحق، پروفیسر، خط بنام ساجد ندیم، یکم جون 2014 ء
(12) عبدالحق، پروفیسر، اقبال اور اقبالیات (کشمیر :سرینگر میزان پبلشر ) بار دوم 2009ء، صفحہ 3
(13) عبدالحق، پروفیسر، خط بنام ساجد ندیم، یکم جون 2014 ء
(14) اقبال کلیات اقبال اردو، بانگ درا، صفحہ 286
(15) اقبال، بکھرے خیالات، مرتبہ، ڈاکٹر جاوید اقبال مترجم پروفیسر عبدالحق ( جون پور یوپی ۔ پہاڑ پو
مچھلی شہر ) مارچ 1975 ء
(16) اقبال، کلیات اقبال اردو، بانگ درا ،نظم، التجائے مسافر صفحہ 122

 

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