Search or add a thesis

Advanced Search (Beta)
Home > Genetic Polymorphism of Bovine Growth Hormone Somatotropin , Kappa-Casein and Beta- Lactoglobulin Genes

Genetic Polymorphism of Bovine Growth Hormone Somatotropin , Kappa-Casein and Beta- Lactoglobulin Genes

Thesis Info

Access Option

External Link

Author

Riaz, Muhammad Naeem

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2013

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2489/1/2567S.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726307659

Similar


Several breeds of buffalo and cattle in Pakistan show mark differences in the milk yield. These animals are in a range of high milk producer to low. A proportion of these differences are explained by the animal genetic make up. In the earlier studies, a number of DNA polymorphisms have been reported in candidate genes. Among these few of candidate genes studied in the present study, are bovine growth hormone (bGH), kappa- casein (k-CN) and beta-lactoglobulin (B-LG) genes using PCR-RFLP methodology. Two genetic variants (A and B) and three genotypes AA, AB and BB have been identified. The genotyping of bovine growth hormone (bGH), kappa casein (k-CN) and beta- lactoglobulin (β-LG) alleles is of practical importance since bGH L, k-CN A & B and β- LG B alleles were found to be correlated with commercially valuable parameters. Genotype information on 366 animals from four breeds including one buffalo and three cattle breeds of Pakistan regarding polymorphisms located in the candidate genes was compared to literature reports. The results from frequency analysis revealed minor deviations from previous reports. Allele frequencies were calculated by gene counting methodology which shows the high prevalence of bGH genotype LL among cattle, while few animals had the LV variant and there was no VV variant was found. A Chi-Square test analysis of studied population of Pakistani cattle for bGH gene indicated that the studied population was in Hardy Weinberg equilibrium. Among cattle two alleles A and B in two genotypic forms AA and AB were found for κ-CN gene fragment. The frequency of allele A was found to be significantly higher in cattle whereas AB genotype was more frequent in Sahiwal cattle. No BB genotype was detected in any of these cattle. The Chi-Square test revealed that observed frequencies and those expected under Hary- Weinberg were not significant at (P< 0.05), suggesting that Cholistani and Red Sindhi population was in equilibrium for k-CN locus, while animals of Sahiwal cattle were not in Hary-Weinberg equilibrium. Analysis of β-LG gene, genotypes AA and BB were detected and no AB genotype was found in the tested animals of Nili-Ravi buffaloes while the frequency of AA genotype was higher. In cattle observed genotypes were AB and BB and the frequency of β-LG allele B was significantly higher in the analyzed animals of three cattle breeds. However, the important thing is the homozygosity found in Nili-Ravi buffaloes for bGH and κ-CN genes and this could be due to the loss of genetic variability among the studied population. Nucleotide sequencing from Nili-Ravi buffaloes shows maximum homology with Bubalus bubalis and Bos taurus breeds. On comparison of amplified gene sequence of Nili-Ravi buffaloes with Bubalus bubalis and Bos taurus breeds show variation at several sites. Thus results show that the PCR-RFLP analysis is easy, cost effective method which permits easy characterization of bGH, κ-CN and β-LG genes even in the absence of their expression. This will facilitates the selective breeding programs aimed at increasing the frequency of desirable alleles.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

اے ڈی اظہر

اے۔ ڈی اظہر(۱۹۰۰۔۱۹۷۴ء) کا اصل نام احمد دین ہے۔ لیکن اے۔ ڈی اظہر کے نام سے ادب کی دنیا میں شہرت پائی۔ آپ سیالکوٹ کے ایک چھوٹے گاؤں ڈگرخورد میں پیدا ہوئے۔ آپ اردو کے ممتاز شاعر ‘ ادیب اور ماہرِ لسانیات تھے۔ آپ ملٹری اکاؤنٹس میں اعلی سرکاری افسر‘ سفارتکار‘ وزیر اقتصادیات‘ رکن قومی ترانہ انتخاب کمیٹی اور ہائی کمشنر آسٹریلیاجیسے عہدوں پر فائز رہے۔ اظہر کے والد ڈی۔ جی پاکستان ٹیلی ویژن رہے۔ اظہر شروع میں شاعری سے زیادہ صرف و نحو میں دلچسپی رکھتے تھے۔ عربی‘ فارسی اور کلاسیکی ادب پر اظہر کی وسیع نظر تھی۔ آپ اردو زبان سے دلی محبت‘ فکری مسائل و تحقیقی مہمات سے گہرا شغف اور پنجاب کی زندگی اور روایات سے والہانہ عشق رکھتے تھے۔ (۳۰۴)

اظہر کے تین شعری مجموعے ’’لذتِ آوارگی‘‘ ’’گریۂ پنہاں‘‘ اور ’’احوال واقعی‘‘ شائع ہو چکے ہیں لیکن انہیں ’’لذتِ آوارگی‘‘ کی وجہ سے شہرتِ دوام ملی ۔حفیظ جالندھری اظہر کے ادبی استاد اور دوست تھے۔ وہ ’’لذتِ آوارگی‘‘ پر منظوم تبصرہ کرتے ہوئے کہتے ہیں:

دیدہ ور اظہر بزورِ بازوئے نظارگی

 

7ہے بغلگیرِ عروس لذتِ آوارگی

 

عمر کے اس مرحلے میں جلوہ ھائے رنگ رنگ

 

فکرِ اظہر سے نظر آنے لگے یکبارگی

 

لذتِ آوارگی اس کو نہیں ملتی حفیظ

 

جس کے ہاتھوں پر لکھی ہو بندگی بے چارگی

(۳۰۵)

اظہر کی تخلیقات کا دامن خود ان کے ظرف کی کشادہ اور ان کی زندگی کی طرح متنوع ہے۔ ان کی تخلیقات جدید و قدیم کا حسین امتزاج ہیں۔ اظہر نے اپنے کلام کو اپنی علمیت کے...

DEPRESSION AND PSYCHOLOGICAL WELL-BEING AMONG PEOPLE HAVING COVID-19 UNDERGONE QUARANTINE

Aims Of Study: Coronavirus is a potentially deadly disease that mostly affects human lung tissue. Multiple cases of unexplained respiratory tract infections were reported to the World Health Organization China in December 2019. The focus of this research was to assess association between depression and PWB in patients who had been isolated due to covid-19. Methodology: A sample of 250 people with covid-19 post quarantine, with age ranges from 25 to 45 were selected by using purposive sampling methods. Depression, anxiety, stress scale and psychological well-being scales were administered. Regression analysis and t-test were employed for statistical analysis. Results: Obtained results evidenced that depression is significantly predict psychological health in the covid-19 population [B= -.015, -.019, F (7.915) sig= .000]. Gender difference is also observed in variable of depression also [(4.78) =.030, p.000]. Limitations and future implications: Future research may base on longitudinal analyses focusing on familial and social factors that may influence the psychological well-being of people living in quarantine. Originality: I certify that the intellectual substance of this article is the result of my own effort and that all assistance and sources used in the preparation of this article have been acknowledged. Conclusion: It was concluded that Depression affects people with COVID-19 and PWB after quarantine. future research may focus more on the influence of the interaction between quarantine and adults’ mental health to fully comprehend the link. 

Comparative Study of Organizational Commitment, Morale and Job Satisfaction of Subject Specialists and Lecturers Teaching at Intermediate Level

This study was aimed at comparing the organizational commitment, morale and job satisfaction of Subject Specialists and Lecturers teaching at intermediate level. The major objectives of the study were to compare the level of organizational commitment, morale and job satisfaction of Subject Specialists and Lecturers and also to determine the relationship among organizational commitment, morale and job satisfaction of Subject Specialist and Lecturers. The differences in the level of organizational commitment, morale and job satisfaction of Subject Specialists and Lecturers were calculated and relationship among their organizational commitment, morale and job satisfaction was found. The study was delimited to Subject Specialists of Government Higher Secondary Schools and Lecturers of Government Colleges working on regular basis, in the jurisdiction of the Khyber Pakhtunkhwa province, Pakistan. It was an inferential study and the survey method was used for data collection. The population for the study was all the Subject Specialists of 292 Government Higher Secondary Schools and the Lecturers of 177 Government Colleges of Khyber Pakhtunkhwa province. Using the single-stage duster sampling technique a sample of 610 Subject Specialists from 95 Government Higher Secondary Schools and 526 Lecturers from 45 Government Colleges was selected. Three instruments i.e. Organizational Commitment Scale (OCS) adapted from Organizational Commitment Questionnaire by Mowday, Steers, and Porter (1979), Morale Scale (MS) adapted from Purdue Teacher Opinionaire used by Houchard (2005) and Job Satisfaction Scale (JSS) adapted from Scale of Job Satisfaction by Bas and Ardic (2002) were used for the collection of data in this study. All the three instruments were validated by seven experts and administered to 145 respondents (74 Subject Specialists and 71 Lecturers) for pilot testing. Data were collected through vitipersonal visits. The number of respondents in this study was 951 (542 Subject Specialists and 409 Lecturers). Data were analyzed by applying two-sample t-test and the Pearson’s correlation test. The major findings of the study were that Lecturers showed a higher level of organizational commitment, morale and job satisfaction as compared to Subject Specialists. There was no difference in level of organizational commitment, morale and job satisfaction between the urban and rural Subject Specialists; the rural Lecturers have higher level of morale and job satisfaction than urban Lecturers while no difference was found in their level of organizational commitment, Both die male and female Subject Specialists as well as Lecturers showed no difference in their level of job satisfaction. Bui male Subject Specialists and Lecturers both showed higher level of organizational commitment than their female counterpart. On the other hand in case of morale, male and female Subject Specialists showed no difference while male Lecturers have higher level of morale than females. The Subject Specialists having more than 7 years experience showed higher level of organizational commitment and job satisfaction than the less experienced Subject Specialists while no difference was found in their level of morale. On the other hand Lecturers showed no difference in all the three variables i.e. organizational commitment, morale and job satisfaction with respect to experience and qualification. The Subject Specialists having only Master degree showed higher level of morale and job satisfaction as compared to those having higher qualification of M. Phil, and Ph, D., while no difference was found in their level of organizational commitment with respect to qualification. A strong positive relationship was found among organizational commitment, morale and job satisfaction for both the cadres of Subject Specialists and Lecturers. It was recommended that enough physical facilities, conducive working ixenvironment and higher qualification allowance might be given to Subject Specialists, there was need to change the physical facilities and working environment in the Higher Secondary Schools and higher qualification allowance may be awarded to Subject Specialists.