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Home > Genetic Studies of Drought Tolerance in Cotton Gossypium Hirsutum L.

Genetic Studies of Drought Tolerance in Cotton Gossypium Hirsutum L.

Thesis Info

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Author

Sarwar, Muhammad

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2013

Thesis Completion Status

Completed

Subject

Botany

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/609

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726311518

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Fifty lines of Gossypium hirsutum L. were screened at seedling stage in glasshouse for drought tolerance. From the germplasm two drought tolerant and two susceptible lines showing genetic divergence will be identified and crossed to obtain hybrid seed . The hybrid seed was planted to develop F 1 generations. Some of the plants from F 1 generation were selfed for F 2 and some back crossed to both the parents (P 1 and P 2 ) to develop seed for back crosses (B 1 and B 2) . All the six generations, P 1, P 2 , F 1 , F 2 , B 1 and B 2 were studied in field under normal and water stressed conditions using completete block design with three replications. During the crop season, water stress will be developed by supplying 50% less irrigations than the normal. Data was recorded on different plant traits related to drought tolerance, yield and fiber quality. The inheritance pattern of various traits was studied using generation means analysis technique. Estimates of narrow sense heritability and nature of correlation among various traits was examined. There were significant differences among six generations (P 1 , P 2 , F 1 , F 2 , B 1 , B 2 ) of two crosses for all the studied plant traits of crosses NIAB-78 × CIM-446 and CIM-482 × FH 1000 under both normal all drought conditions. Generation means analysis indicated additive, dominance and epistatic genetic effects played role in the inheritance of all the traits under both normal and drought condition. Two parameter model [md] provided best fit of observed to the expected generation means for number of bolls per plant under normal conditions in cross NIAB-78 × CIM-446 and for number of monopodial branches of the same cross under drought conditions. In case of cross CIM-482 × FH- 1000 two parameter model [md] was found fit for Fiber fineness under normal conditions. The dominace or dominace × dominance effects were observed for some traits in both the crosses under both normal and drought conditions. Some plant traits showed [i], [j] and [l] type of interactions together which indicated complex inheritance of these traits. In the generation variance analysis only additive effects were involved in the inheritance of most studied plant traits but generation means analysis showed that additive, dominance and epistatic effects were involved in the inheritance of these traits. The narrow sense heritability estimates of infinity generation (F∞) were consistently higher than F 2 generation. High narrow sense heritability estimates 0.67, 0.66 and 0.65 were observed for number of sympodial branches, number of bolls per plant and seed cotton yield, respectively for cross-1 (NIAB-78 × CIM-446) under normal conditions and narrow sense heritability estimates 0.79, 0.69 and 0.58 were observed for boll weight, seed cotton yield and relative leaf water content respectively under drought conditions for cross-1. Seed cotton yield had positive significant correlation with boll weight, fibre length, fibre strength, lint percentage and relative water content except fibre fineness, exised leaf water loss, leaf temperature and leaf area in cross-1 (NIAB-78 × CIM-446) under normal and drought conditions and in cross-2 (CIM-482 × FH-1000) under normal conditions. The information derived from these studies will provide guideline to cotton breeders in breeding of drought tolerant cotton cultivars.
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حضرت خدیجہ ؓ سے شادی اور ان کا کاروبار

حضرت خدیجہؓ سے شادی اور ان کا کاروبار
اعتراض نمبر۹۰
جب آپ ؐ جوان ہوگئے اور مضبوط ہوگئے( آپ کے پاس زیادہ مال نہ تھا) سیدہ خدیجہؓ نے آپﷺ کو تجارت کے لیے اجرت پر بلالیا۔۔اور حضور ؐ کے ساتھ ایک اور آدمی کو اجرت پر رکھ لیا‘‘ ( اردو ترجمہ دلائل نبوہ ۱۔۶۴۔۔ ابوبکر احمد بن الحسین بیہقی)
’ تاریخی کتب سے یہ تاثر ابھرتا ہے کہ نبی مکرمﷺ کو سیدہ خدیجہؓ نے گویا اپنا ملازم یا تجارتی کارندہ مقرر کیا تھا‘‘ ( سیرت اعلان نبوت سے پہلے۔۲۹۵۔۲۹۴)
جواب:صاحب ضیا النبی( ۔۲۔۲۱۷) لکھتے ہیں کہ آپ( خدیجہ) اپنے نمائندوں کو سامان تجارت دے کر روانہ کرتیں‘ جو آپ کی طرف سے کاروبار کرتے۔ اس کی دو صورتیں تھیں ۔
اول: یا تو ملازم ہوتے، ان کی اجرت یا تنخواہ مقرر ہوتی جو انھیں دی جاتی‘ نفع و نقصان سے انھیں کوئی سروکار نہ تھا۔
دوم: یا نفع میں ان کا کوئی حصہ ،نصف یا چہارم مقرر کر دیا جاتا۔ اگر نفع ہوتا تو وہ اپنا حصہ لے لیتے ۔بصورت دیگر یعنی نقصان کی صورت میں ساری ذمہ داری خدیجہؓ پر عائد ہوتی ‘ اس کو شریعت میں’’ عقد مضاربہ‘‘ کہتے ہیں۔ آنحضرت ایک خود مختار اور خوش حال تاجر تھے۔ آپ نے کسی تاجر کی ملازمت کبھی اختیار نہیں کی تھی‘ ڈاکٹر تحسین فراقی نے کتاب کے تعارف میں لکھا ’’ ایک عام تاثر یہ پایا جاتا ہے کہ حضرت خدیجہ نے حضورؐ کو اپنا ملازم یا تجارتی کارندہ مقرر کیا تھا۔حالانکہ حقیقت یہ ہے کہ حضرت خدیجہ ؓ سے نکاح سے بہت پہلے آپؐ خود مختار اور خوش حال تاجر کے طور پر معروف ہو چکے تھے اور اس ذیل میں قیس بن السائب کی روایت استناد کا درجہ رکھتی ہے۔
ایک اور روایت:ابو طالب نے اپنے بھتیجے سے کہا ’’ میں...

مشاركة المسلم في الأعمال السياسية بالبلاد غير الإسلامية وحكمها

In this paper, the question of the participation of the resident Muslim in the foreign countries in politics and its rulings and the statement of some issues that are presented to the Muslim, such as: to elect, run or participate in political parties and other issues related to this topic. And also, tried to study the views of the scholars, and presented them and their attitudes, evidence in them, compared with them. The modern world has witnessed phenomena, which is not hidden to every sane person, which was, the Muslim participation in the political activities in the non-Muslim countries. The importance of this research paper is clear because it reveals the meaning of the political concept in the Holy Quran and prophetic tradition, which made the researcher in the attempt to legalize the matter in this regard and to summarize the statements of ancient and contemporary scholars based on their arguments and evidences. The study concluded That the participation in politics activities non-Muslims societies does not fall within the scope of the doctrine originally, since no one doubts in the doctrine of Muslims that it is not permissible to resort to the tyrant and not to judge except with Allah almighty revealed rules and regulations, and when this doctrine disappears or enter doubt of any act of apostasy and disbelief, which is not satisfied by any sound Muslim at this very moment that falls into haram category. At the end conclusion is drawn from variant views of the scholars, and the main findings and recommendations have been given.

Phyto- Availability of Cadmium to Rice and Wheat in Contaminated Soils Treated With Organic Amendments

Cadmium (Cd) pollution is an emerging concern in many industrialial areas, mainly where raw effluents released from industry and cities. In many developing countries like Pakistan these effluents are used to raise grain crops and vegetables despite someof theses effluents having higher levels of metals such as Cd. In this thesis we investigate Cd availability and accumulation in wheat and rice following a series of four experiments. The first experiment was a hydroponic study executed to explore the effect of Cd on growth and micronutrient accumulation in wheat and rice. Cadmium was applied in three levels i.e. 5, 10 and 15 µM and nutrient solution was Hoagland. Results indicated that growth of both wheat and rice was significantly affected at higher Cd (15 µM) level. Increasing Cd levels significantly reduced gas exchange characteristics including photosynthetic rate and transpiration rate of both crops. Root and shoot Cd contents were highest, whereas micronutrient (Cu, Fe, Mn and Zn) concentration was minimum in 15 µM Cd treated plants of both crops. On the basis of solution culture experiment it was concluded that, Cd has significant effect on growth, physiological and micronutrient contents of wheat and rice especially at 15 µM Cd application. The second experiment was a pot study to evaluate the effectivesness of different organic amendments on reducing Cd availability to rice and wheat in an artificially Cd contaminated soil. Amendments used were cotton sticks biochar (CSB), wheat straw biochar (WSB), rice husk biochar (RHB), farm yard manure (FYM), poultry manure (PM) and press mud (PrMd) @ 2% (w/w). The results revealed that addition of organic amendments significantly improved growth and reduced shoot, root and grain Cd and AB-DTPA extractable soil Cd in wheat-rice cropping. The maximum Cd reduction in soil and plant was recorded with the application of PrMd and RHB in both crops whereas the highest Cd concentration was observed in unamended Cd applied soil. On the basis of this study, it was concluded that, addition of organic amendments especially PrMd and RHB improved growth and yield of both crops and reduced Cd uptake. The third trial was accomplished to study the role of selected organic materials on Cd phyto-availability and growth of wheat and rice in raw effluent irrigated farmer’s field at village Suraj Miani, suburbs of Multan city. Treatments were control; RHB; FYM; PM and PrMd @ 10 Mg ha-1 randomized in 9 m2 sized plots. The results indicated that RHB and PrMd showed higher gas-exchange characteristics and lower plant and AB-DTPA extracable soil Cd. The soil (0-15 cm) Cd was lowered by 33 and 32% in post-wheat whereas 48% in post-rice by the application of RHB and PrMd respectively over control. The economics of treatments showed that except RHB, addition of amendments gave higher benfit cost ratio over control. Among amendments FYM was found more economical followed by PM and PrMd. The fourth experiment was performed in pots to study the effect of organic acids on Cd availability to wheat and rice. Organic acids viz. oxalic acid (OA), citric acid (CA), EDTA and tartaric acid (TA) were applied @ 2 mM before sowing of wheat. Rice seeedlings were transplanted to similar (post-wheat) pots to observe the long term effectiveness of applied organic acids. The results showed that Cd in root, shoot and grain/paddy of wheat and rice was increased by addition of organic acids and found highest for EDTA as compared to rest of treatments. The post-harvest AB-DTPA extractable soil Cd was also higher with EDTA treatment. Over all, Cd stress reduced growth and yield of both wheat and rice in natural and Cd spiked growth medium. This shows that Cd stress is inversely related with growth, yield and micronutrient uptake, whereas Cd uptake and concentration is directly related with Cd concentration in growth medium. Application of organic amendments especially PrMd and RHB improve growth, yield and photosynthetic attributes of both crops in artificially (spiked) Cd contaminated soil and raw sewage irrigated field as a result of less Cd uptake and translocation. Addition of organic acids especially EDTA formed soluble chelates with Cd thus increased Cd phytoavailability and uptake by wheat and rice.