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Genetics of Morpho-Physiological Traits in Wheat under Normal and Drought Conditions

Thesis Info

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Author

Irshad Ahmad

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2014

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2414/1/2845S.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726325247

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Six bread wheat cultivars/lines viz., Faisalabad-08, Lasani-08, Sehar-06, Chakwal-86, Chakwal-50 and 6302 were hybridized in full diallel fashion. Thirty F1 crosses along with six parents were planted in a triplicate randomized complete block design in the experimental area of Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad. Experiment was sown under both normal and drought conditions. Data on attributes viz., plant height, flag leaf area, number of tillers per plant, number of spikelets per spike, number of grains per spike, 1000-grain weight, spike length, spike density, grain weight per spike, biomass per plant, grain yield per plant, harvest index, extrusion length, stomatal frequency, stomata size, epidermal cell size and leaf venations were recorded and subjected to the analysis of variance technique to find out the genotypic differences for the characters, which were found significant under both environmental conditions. Data were further subjected to diallel analysis for genetic studies. The Vr-Wr graphs indicated over-dominance type of gene action for spike length and grain weight per spike and leaf venations under both sowing conditions and for flag leaf area, biomass per plant, harvest index and stomatal frequency under normal condition, whereas for number of tillers per plant under drought condition. Additive with partial dominance type of gene action was observed for number of spikelets per spike, number of grains per spike, plant height, 1000-gran weight, grain yield per plant, extrusion length, spike density, stomata size and epidermal cell size under both sowing conditions and for number of tillers per plant under normal condition and for biomass per plant, harvest index, stomatal frequency and flag leaf area under drought condition. Over-dominance type of gene action detected under normal condition for flag leaf area, biomass per plant, harvest index, and stomatal frequency was changed to additive with partial dominance type of gene action under drought condition, while additive with partial dominance gene action under normal condition for number of tillers per plant was altered to over-dominance type of gene action under drought condition
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سلطان کھاروی دی گیت نگاری

سلطان کھاروی دی گیت نگاری

’’گیت ہندی زبان دالفظ اے جیدے معنی نیں راگ، سنگیت، بھجن ، سرور وغیرہ۔‘‘(۱)

ایس لئی گیت تے سنگیت دا آپو وچ گوہڑا سمندھ اے جتھے گیت اے اوتھے سنگیت اے تے جتھے سنگیت ہووے گا اوتھے گیت وی ہووے گا۔ گیت دراصل من دیاں ڈوھنگایاں چ جنم لین والے جذبیاں تے احساساں دے آپ مہارے پر گھٹاوے داناں اے ۔ جیہدے وچ سادگی، سلاست تے روانی مکمل درجے تک موجود ہوندی اے۔ ایسے پاروں آکھیا جاندا اے کہ گیت کوتا دا اک اجہیا روپ اے جو دلاں دیاں اتھا ڈوھنگایاں وچ پیدا ہوندی اے، تے دلاں دیاں اتھاہ ڈوھنگایاں تیک اثر کردی اے ۔ گیت کیول جذبیاں دے اظہار دا ناں ہی نہیں بلکہ ایہدے سرنانویاں وچ بڑی وسعت تے ون سونتا پائی جاندی اے۔نظم وانگوں گیت نوں وی خاص موضوع تیک محدود نہیں رکھیا گیاسگوں نظم جذبہ تے احساس دیاں نکی توں نکی کیفیتاں گیت دا موضوع بن سکدیاں نیں۔ ایس لئی عشق تے محبت ، کوشش تے محنت ، قومیت ، حریت رزم تے بزم ، کرم تے ظلم ، ہجر تے وصال ، جذبہ تے خیال، حسن تے جمال، عشق بے مثال ،ہراوہ شے جیڑی انسانی احساس دا حصہ اے گیت دا موضوع بن سکدی اے۔گیت بارے ڈاکٹر وزیر آغا لکھدے نیں:

’’گیت کا امتیازی وصف یہ ہے کہ ماں ، زمین یا معاشرے کے باطن میں پیدا ہونے والی کروٹ کا علم بردار ہے۔ اسی لیے گیت میں زمین سے وابستگی بہت توانا ہے۔ مثلاًگیت کی آواز میں دھرتی کی بہت سی دوسرے آوازیں شامل ہوجاتی ہیں۔ جسے کوئل کی کوک،مینا کا ترنم، بھنورے کی گھن گھن وغیرہ۔(۲)

سلطان کھاوری پنجابی زبان دے اچ کوٹی دے کوی نیں...

حضرت محمد ﷺ کے معاشرتی رویے اور ان کے نفسیاتی اثرات

The social life of an individual is reflected in his behaviors and attitudes. These attitudes are the bedrock of our social life and determine our thinking and actions. These attitudes Colors our thinking in positive or negative way. The life and biography (See’rah) of the Holy Prophet (saw) serves as a model for us to fashion our behaviors. The enlightened teachings of the Holy Prophet (saw) established a balanced relations ship between individual and social development. This essay analysis patterns of social behaviors in the light of the See’rah Holy Prophet (saw).

Characterization of Fungal Pathogen S Causing Wilt of Lentil and Their Management

Vascular wilt of lentil caused by various ecologically and phylogenetically diverse species of Fusarium is found in all the lentil growing areas of Pakistan and the disease could be visualized at both seedling and adult stages of plant growth. The disease is responsible for huge losses each year in Pakistan, yet, there is a scarcity and lack of literature and information regarding its occurrence, incidence, distribution, biology and management of wilt pathogens. Therefore, the study was planned keeping in view the national interests to avoid future losses caused by lentil wilt. The objectives of this study were to assess the wilt prevalence and incidence in major lentil growing districts of Punjab, morpho-molecular and pathogenic characterization of recovered wilt pathogens and the management through host plant resistance, biological control agents and fungicides. A two year field survey data (2011-12 and 2012-13) and laboratory isolations ascertained 213 isolates of Fusarium pathogen as associated wilt incidence identified in the fields. Disease was found widespread with 100% prevalence in all the major lentil growing districts of Punjab viz. Bhakkar, Layyah, Mianwali, Khushab, Sialkot, Narowal, Chakwal, Attock, Gujrat and Jhelum. The mean wilt incidence was found 28% with maximum incidence recorded at adult plant stage (32.4%) than at seedling (23.05%). Morphological characterization showed significant variation among the isolates and based on similar morphology, these were grouped into 67 type isolates for subsequent study. The in vitro pathogenicity testing through root dip method using line NARC-08-1 and cultivar Masoor-93 showed excellent production of wilt symptoms for pathogenic characterization. High pathogenic variability was revealed among the isolates. Based on disease reaction i.e. avirulent to highly virulent observed on most susceptible line NARC-08-1, isolates showed 0 to 100% disease incidence and severity index with significant (11.86 to 100%) reduction in yield. The isolates were grouped into four categories viz. highly virulent (13 isolates, 19.40%), moderately virulent (41, 61.19%), low virulent (8, 11.94%) and avirulent (5, 7.46%). The highly virulent isolates included FWC15, FWJ35, FWJ49, FWG1, FWS11, FWS13, FWN2, FWL2, FWL6, FWL9, FWL12, FWB10 and FWK2. Molecular characterization and DNA sequencing of isolates through PCR amplification of translation elongation factor TEF-1α gene region using primers ef1 and ef2 confirmed the identity of the Fusarium isolates at species level. The amplification produced a single DNA fragment of size 700bp in each of the isolates. Phylogenetic analysis of 67 morphologically and pathogenically diverse Fusarium isolates recovered from various lentil districts of the country revealed that the isolates belonged to different clades under five distinct species. The identified species included F. oxysporum, F. redolens, F. nygamai, F. commune and F. equiseti. This data supported the morphological variation observed among the isolates and divulged the association of these identified species in wilt disease incidence as reported in the major lentil producing region of the country. The findings revealed the highest prevalence of F. oxysporum (49.29%) in the region followed by F. redolens (29.57%), F. equiseti (10.79%) and F. commune (7.98%), while least prevalence was of F. nygamai (2.34%). The most virulent F. oxysporum isolate FWL12 (GenBank accession number KP297995) was selected for the management trials. Screening of the lentil germplasm revealed reduced wilt infection in five cultivars viz. Markaz-09, Masoor-86, Masoor-2006, Punjab Masoor-00518, Punjab Masoor-09 that showed 4.44 to 12.59% severity index, 20 to 46.67% incidence and 9.60 to 24.94% yield reduction. The biological management revealed the best efficiency of T. harzianum in reducing wilt infection on line NARC-08-1 and produced 8.9% disease severity index, 26.7% incidence with 16.27% yield reduction. Chemical seed treatment identified systemic fungicides as highly effective in disease management that resulted in improved 100% seed germination. Benomyl (6.7% incidence and 1.5% severity index) was found superior than Thiophanate methyl (13.3%, 3%). In conclusion, the results of this research study provide an overall current status of wilt disease in the country and high lightened the areas under current high risk of its spread. The findings also revealed the continuous reduction in the acreage of this crop in the major lentil region. The revealed association of five virulent and morpho-molecularly diverse species viz. F. oxysporum, F. redolens, F. nygamai, F. commune and F. equiseti with the wilt disease is reported for the first time in Pakistan. The screening suggests five cultivars viz. Markaz-09, Masoor-86, Masoor-2006, Punjab Masoor-00518 and Punjab Masoor-09 as an important source of resistance for lentil breeding against wilt. Moreover, T. harzianum proves an efficient biological control agent, while seed treatment suggests Benomyl and Thiophanate methyl as the most effective against the wilt pathogen.