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Genetics of Water Deficit Tolerance in Gossypium Hirsutum L.

Thesis Info

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Author

Chattha, Waqas Shafqat

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/8394/1/Waqas_Shafqat_Chattha_2017_HSR_PBG_UAF_09.11.2017.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726328349

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Increasing water shortage for irrigation is a major constrain to sustainable cotton production. Cotton plant observes reduction in seed cotton yield as well as fibre quality when subjected to water deficit conditions. Present investigation were conducted to study the genetic basis of agro-physiological and fiber quality traits in cotton under normal irrigation and water deficit stress. For this purpose, 45 genotypes of Gossypium hirsutum L. were studied under two moisture levels i.e. normal irrigation and water-deficit stress conditions for two years (2013 and 2014). The experiment was conducted using split plot arrangement under randomized complete block design. All the genotypes behaved differently under both control and water - deficit stress. Genotype × Environment Interaction (GEI) of cotton genotypes with two water levels (Environments) were studied for some selected agro-physiological traits i.e. seed cotton yield, bolls per plant, boll weight, water potential, osmotic potential, pressure potential, chlorophyll fluorescence, canopy temperature, relative water contents, protein contents and proline contents using AMMI biplot analysis. Results showed that the genotypes VH-291, FH- 329, FH-153, IR-6, FH-159, VH-289, FH-322, MNH-886, S-15 and FH-207 proved stable for most of the traits studied whereas; NS-131, AA-703 and KZ 191 interacted undesirably with water-deficit stress. Results of screening revealed that working variability among germplasm was present and controlled by significant genetic component, which may be exploit through hybridization. Therefore, F1 crosses were developed in the greenhouse keeping stress tolerant genotypes as lines and sensitive as a testers using line × tester technique. 30F1 crosses along with 13 parents were planted under two stress levels i.e normal and water-deficit in field condition to examine the genetic basis of agro-physiological and fiber quality traits. Under normal condition lines showed more contribution for bolls per plant, seed cotton yield and canopy temperature whilst, line × tester interaction showed maximum contribution for boll weight, water potential, osmotic potential, pressure potential, staple length, fibre fineness and fiber strength. Under water-deficit condition, the expression of bolls per plant, seed cotton yield, osmotic potential, pressure potential, relative water contents, protein contents, staple length, fiber fineness and fiber strength were largely determined by interaction of line × tester. Contribution by lines were more for boll weight, water potential and canopy temperature. The analysis of F1 and parents data revealed the presence of non-additive genes controlling variation under both normal and water-deficit condition. FH-159 and FH-207 were best general combiners under normal irrigation and water deficit condition. Under normal condition, cross FH-322 × NS-131 showed best specific combining ability while FH-207 × KZ-191 was best combination under water deficit condition for most of the traits. Higher heterosis over better parent were observed in FH-159 × KZ-191 and VH-289 × AA-703 under normal and water deficit condition respectively. For seed cotton yield the crosses FH-207 × NS-131, S-15 × AA- 703 and FH-329 × NS-131 showed higher and significant heterosis over better parent under water deficit condition that may be used in development of hybrid for water shortage areas in Pakistan.
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میں تشنہ لب ہوں آنکھوں میں مگر ساون تو رکھتا ہوں

اگرچہ تیرگی ہی تیرگی ہے چار سُو میرے
میں ایسی تیرگی میں بھی دلِ روشن تو رکھتا ہوں

مرے دامن میں رنج و غم کی ہے اشکوں کی دولت ہے
مجھے مت جانیے مفلس کہ ایسا دھن تو رکھتا ہوں

میں بستا شہر میںہوں پھر بھی صحرا سے مجھے نسبت
نہیں میں قیس تو کیا ہے میں پاگل پن تو رکھتا ہوں

میں اُن کو دل میں رکھتا ہوں جو تائبؔ دور رہتے ہیں
اگرچہ بے ہنر ہوں پھر بھی اتنا فن تو رکھتا ہوں

اسلام اور جین مت میں طہارت کا تقابلی جائزہ

      Allah created all the required things fo the world since the world was given existence. Human was made its tenant and was equipped with art of living and eversince human life exists in all the corners of the world whatsoever religion they follow, but  they do follow a set of social principles in life. And literal meaning of those principles is called religion, because religion literally mean"way of living or manner of living". And contextually religion may be defined as the principles followed to live life.       In the light of this definition of religion some people have been confronted having no concept  of  religion, they are called Communists. But anyone who does not believe in Allah and the Prophet PBUH are termed Communists. In the following dissertation a comparison has been drawn between Islam and jeen Mat to show that no religion can compete Islam in terms of Clarity or cleanliness.

Variability Among Ralstonia Solanacearum Isolates and Screening of Chili Germplasm for Resistance

Chili (Capsicum annuum) belongs to family solanaceae and is one of the most common cultivated crops of the world. Chili has been cultivated on 63.6 thousand hectares in Pakistan with a production of 147.2 thousand tons in 2012-13.Bacterial wilt caused by Ralstonia solanacearum is a highly challenging and destructive disease of solanaceous crops. R. solanacearum is a soil and water borne bacterium which enters the plant roots, multiplies through the xylem, and collapses the host. The bacterial wilt infects more than 450 plant species belonging to 54 different botanical families. Major hosts include tomato, hot pepper, sweet pepper and potato. Eighty countries are affected with a loss of $ 1 billion each year. As the information regarding distribution of bacterial wilt in different agro-ecological zones of Pakistan, pathogenic variability among different isolates, genetic diversity of the bacterium and response of different chili cultivars towards the pathogen is lacking therefore the present studies were carried out. Major chili growing areas from different agro-ecological zones were surveyed for the determination of incidence, prevalence, biovars identification, pathogenic variability and genetic diversity of different isolates of Ralstonia solanacearum. Chili germplasm comprising 28 varieties was screened for their relative resistance or susceptibility against the highly virulent strain (RsBd 6) of the bacterium in the glasshouse. The maximum incidence of 22% of bacterial wilt was recorded in district Badin while the incidence was the minimum (4.4%) in district Loralai. The disease incidence was found to be the maximum (19.5%) in Indus delta and was the minimum in Western dry mountains (5%). Maximum incidence was observed in Sindh province followed by Punjab (11.4%) and was the minimum in Baluchistan (4.9%). The xxi overall incidence in the country was 9.95%. The prevalence was found to be the maximum in Sindh (100%) followed by Punjab (84%) with an overall prevalence of 75.8% in the country. A total of 114 isolates of R. solanacearum from eight agro-ecological zones were isolated, confirmed by immunoStrips and characterized by employing different biochemical tests. The biovars were identified on the basis of sugar consumption. Of the 114 isolates, 92 were distinguished as biovar 3 while 22 were recognized as biovar 4. Both the biovar 3 and biovar 4 were diagnosed from Sindh and Punjab while only biovar 3 was distinguished from Baluchistan and Khyber Pakhtoonkhwa. The isolates varied in pathogenicity when tested on highly susceptible cv. California wonder; 21.9% isolates were found highly virulent, 29.8% virulent, 25.4% weakly virulent and 22.8% were avirulent. The isolates were then confirmed through PCR by using specific primers and running on 1% agarose gel visualized under UV light. The screening of chili germplasm against the bacterium revealed that two cultivars viz. Skyline II and Hifly were highly resistant. Sanam was the only cultivar which was identified as resistant. Five cultivars were categorized as moderately resistant. The cultivars Maxi and Talhari were found highly susceptible to the pathogen while rest of the varieties was either susceptible or moderately susceptible. It is concluded that bacterial wilt caused by R.solanacearum is prevalent throughout the country in all the agroecological zones with varying intensities warranting stringent surveillance and control measures. As variations have been observed in the virulence of R. solanacearum isolates, management strategies should be followed accordingly. Resistant and moderately resistant cultivars have been identified, therefore, recommended for cultivation.