This dissertation has been divided into three chapters. Each chapter has its own references. The chapter 1 describes synthesis, characterization and bioassay screening of sixtytwo (62) Schiff bases of different aldehydes (ᵴƀ1-ᵴƀ55) and ciprofloxacin (cp1-cp7) with primary amines by both, conventional as well as ultrasonication methods. Thirteen Schiff bases (ᵴƀ6, ᵴƀ11, ᵴƀ17, ᵴƀ22, ᵴƀ23, ᵴƀ24, ᵴƀ28, ᵴƀ29, ϲρ3, ϲρ4, ϲρ5, ϲρ6 and ϲρ7) are new derivatives. Comparative analysis of these two methods was also carried out. The structures of all synthesized compounds were determined with the help of IR, 1H-NMR, 13C-NMR and EI-MS spectroscopy techniques. All synthesized compounds subjected to different biological activities such as Antiinflammatory activity, Insecticidal activity, Urease Inhibitory Activity, Tyrosinase Inhibitory Activity, Anti-bacterial activity, Anti-fungal activity and Anti-oxidant activity. Only eight compounds showed tyrosinase inhibitory activity in which four compounds ᵴƀ33, ᵴƀ49, ᵴƀ50 and ᵴƀ51 were found most active and the rest of four compounds ᵴƀ32, ᵴƀ34, ᵴƀ38 and ᵴƀ55 were moderately active. ϲρ is active against tyrosinase enzyme but its derivative ϲρ6 was more active. Total eight compounds were found active in urease inhibitory assay. Five compounds ᵴƀ35, ᵴƀ38, ᵴƀ41, ᵴƀ52 and ᵴƀ54 exhibited good activity and rest of three ᵴƀ33, ᵴƀ37 and ᵴƀ49 were moderately active. ϲρ and its derivative ϲρ1 and ϲρ2 were also active in urease inhibitory assay. The compounds ᵴƀ1, ᵴƀ13, ᵴƀ41 and ᵴƀ49 were found significantly active against nearly all applied bacterial strains, ϲρ and its all derivatives (ϲρ1-ϲρ7) are active against many bacterial strains. It is found that Compounds ᵴƀ1 and ᵴƀ2, ᵴƀ13, ᵴƀ38, ᵴƀ40 to ᵴƀ43 showed strong activity against various fungal strains. The synthesized compounds were also tested for anti-oxidant activity. They displayed varying % inhibition, among them ᵴƀ3, ᵴƀ6, ᵴƀ11, ᵴƀ22, ᵴƀ24, ᵴƀ29, ᵴƀ30, ᵴƀ37, ᵴƀ38, ᵴƀ43, ᵴƀ48 and ᵴƀ52 exhibited remarkable anti-oxidant activity. ϲρ, ϲρ1 and ϲρ6 also displayed anti-oxidant activity. ϲρ6 exhibited insecticidal and immunomodulatory/anti-inflammatory activity while Unfortunately, all candidates were found inactive towards both activities. The chapter 2 describes Synthesis of nanoparticles of different metals by various methods. Three types of nanoparticles were synthesized, metal, metal oxide and metal ferrite. Metal nanoparticles were synthesized by Chemical Reduction Method, (Green synthesis) by consuming Cucumis sativus, Azadirachta indica and Syzygium aromaticum aqueous extract and egg white as a source of albumin. Metal nanoparticles of Fe (INP), Ni (NNP), Cu (CNP), Zn (ZNP), Pb (LNP), Mg (MNP) and Co (CTNP) were synthesized. For the synthesis of metal ferrite Co-precipitation technique was employed, by this method CuFe2O4 (CFNP), NiFe2O4 (NFNP), CoFe2O4 (CTFNP), PbFe2O4 (LFNP), and MgFe2O4 (MFNP) nanoparticles were synthesized. For metal oxide nanoparticles Sol-Gel Synthesis (using tartaric acid as Complexing agent), Sonochemical synthesis by using Camellia sinensis extracts and by using seaweeds (Hypnea musciformis, Gracilaria, Laurencia pinnatifida and Enteromorpha intestinalis). These seaweeds were first time used to synthesize different metal nanoparticles along with sound waves. Fe3O4 (IONP), NiO (NONP), CuO (CONP), ZnO (ZONP), MgO (MONP) and PbO (LONP) metal oxides nanoparticles were prepared. UV-vis, FTIR, SEM, AFM, EDX and XRD were the characterization techniques used to confirm the synthesis, attachment of capping agent, morphology and size determination. These nanoparticles were employed to check their anti-microbial activity. These nanoparticles were also used with anti-aging / anti-bacterial cream to investigate antimicrobial and anti-oxidant activity. Some nanoparticles were also used to remove lead ion from aqueous solution by Adsorption. Chapter 3 deals about overview of biological activities and all the procedures used to find the inhibitory power of all synthesized products.
This study examines the association of living areas (slum and non-slum) with the selected public health-related variables in the presence of socioeconomic variables among married women and also having a child. A total of 150 women aged 18 to 49 are selected of which 50 women from slums and 100 women are from non-slum areas of Multan by applying the cluster and random sampling techniques. The cross-tabulation method is used to find the results. The dependent variable is Body Mass Index (BMI) and it is analyzed with the socio-economic variables such as mass media index, household characteristics and education. The findings exhibit that the BMI of the women living in the slum areas is low due to a low level of education, lack of mass media access, bad household structures, and poor or ignorant area. The women of the slum area have fewer mass media access, poor status of household characteristics and less education as compared to the women living in the non-slum areas. BMI is significantly affected by area, women's education and household characteristics except for physical work, job status, mass media access and husband education. The findings of this study suggest that to provide health facilities or to reduce the gap in public health, education, mass media access and households characteristics might be considered while making any decision related to the slum and non-slum areas.
This study was conducted to standardize plant spacing (Exp. # 1), planning geometry (Exp. # 2) and nutrients input (Exp. # 3 & 4) for quality seed production of radish. Field experiment were conducted during 2013-14 and 2014-15. The results of 1st experiment exhibited that though the growth characters like number of branches, branch length, silique length, silique weight, number of siliques/plant, 1000 seed weight and seed yield/plant were maximum in treatment combination of row and plant spacing 75×45 cm, but seed production/plot was highest in treatment combination of row and plant spacing 60×45 cm, due to higher plant population per unit area. Up take up of NPK was maximum for treatment combination of 75×45 cm due to less inter-plant competition. So, maximum seed production can be obtained by adopting the row and plant spacing of 60×45 cm. In 2nd experiment, it was observed that ridge sowing technique was the best for seed production of radish, is optimal. While, Mino Early variety, performance was better than forty days variety in ridge sowing method. Maximum, N, P and K contents in seed were also observed in ridge sowing. Least seed production was recorded in flat sowing. The results obtained from 3rd experiment indicated that the application of N, P and K @ 100: 100: 75 kg/ha was effective for getting higher seed yield of radish under climate of Faisalabad, Punjab, Pakistan. Plant lodging increased at higher application rates of nutrients, especially nitrogen. Maximum N, P and K contents in seed were recorded in response to NPK application @ 125:100:50 kg/ha, 125:75:75 kg/ha, 125:50:75 kg/ha, respectively. There was no significant impact of various fertilizer doses on number of seeds/silique, silique length and seed germination. The results of 4th experiment exhibited stecklings produced from healthy roots ensured higher and uniform seed production of radish crop. Addition of N, P and K @ 75: 65: 65 kg/ha were found effective for getting higher yield of radish root crop, these roots, when subsequently used for steckling preparations, gave higher seed yield than other fertilizer application rates. Plant lodging increased with increase in nutrient application rates, especially nitrogen. There was no significant impact of various fertilizer doses on seed germination. In crux, for highest seed production, steckling should be planted on ridges, keeping row to row and plant to plant spacing of 60×45 cm, and NPK should be applied @ 100: 100: 75 kg/ha under the agro climatic conditions of Faisalabad, Punjab, Pakistan. While, addition of NPK @ 75: 65: 65 kg/ha to root crop for higher root yield and such roots produced higher seed yield than other fertilizer treatments.