The object of this study was to synthesize highly biocompatible and stable gold, silver and copper nanoparticles, without use of toxic reducing and stabilizing agents in aqueous media. In order to achieve this objective, natural polysaccharides (hemicelluloses) were isolated from Plantago ovata (PO), Mimosa pudica (MP) and Lallemantia royleana (LR) and used as reducing and stabilizing agents. The isolated polysaccharides were characterized by elemental, moisture, optical rotation, FTIR and MALDI-ToF analyses. They were found to be highly branched hemicelluloses. It was discovered that the hemicelluloses can effectively reduce noble metal ions due to presence of aldehyde groups in their structures. These hemicelluloses can absorb water several times their mass and swell like hydrogels. In swollen form they can encapsulate and disperse nanoparticles in their matrix, as evidenced by electron microscopy. The dispersion was effective in keeping the particles from each other over longer periods of time. Metal nanoparticles were readily obtained after mixing the metal salts with suspensions of these hemicelluloses in water. The reaction rate followed the order: PO ≥ LR > MP. The reduction process was witnessed by colour change (from yellow to blue/purple/ruby red depending upon the pH and reaction temperature) and monitored by recording the surface plasmon resonance spectra in the 350-800 nm range. The synthesized nanoparticles exhibited spherical shapes. Size of particles was found to depend on amount of hemicellulose used, pH and temperature of reaction mixture. Effect of these parameters was optimized by response surface methodology to get smallest possible size of the particles. The lowest surface plasmon resonance peaks representing smallest size obtained by reduction of gold with MP, PO and LR were 530, 520 and 517 nm, respectively. The SPR spectra of AgNPs started building up at pH > 6 and exhibit maxima at 412, 396 and 387 nm with PO, MP and LR, respectively. The smallest particle sizes obtained under optimum conditions were ~ 10 nm (with LR), ~10 nm (with LR) and 70 nm (with LR), respectively of gold, silver and copper. The synthesized gold nanoparticles were found to be non-toxic as shown by cytotoxic tests. The size of the gold nanoparticles ( > 30) obtained by use of MP falls in the range that is suitable for cellular uptake in various cancer cell lines, as the gold nanoparticles having size > 30 nm can rarely enter nucleus of the cell, so are suitable for safe drug delivery. On the other hand nanoparticles of silver and copper, exhibited dose-dependent antimicrobial activities against E. coli, A.tumefaciens, P. aeruginosa, B. subtilis and S. aureusand, they were found to be cytotoxic against brine shrimps and Hela cell lines and the tumours caused by A. tumefaciens. These results suggest that gold nanoparticles can be used for safe drug delivery without any therapeutic effect of their own, whereas nanoparticles of silver and copper can be used as anti-infective and anti-cancer agents. Gold nanoparticles being non-toxic were subjected to bio-distribution studies in rabbit and tumour bearing mice by use of radio labelled gold (198Au) nanoparticles. The uptakes differed with the use of hemicelluloses. Highest uptake of gold nanoparticles (radiolabelled) dispersed in PO mucilage, after 3h, was found in spleen followed by liver, heart, muscle, kidneys and lungs in rabbit. In case of gold nanoparticles synthesized by use of MP the highest uptake was in spleen followed by liver, kidneys, blood and bladder after 3h. They depleted with time in spleen and were significantly higher in liver after 24h. The particles also accumulated in epithelium carcinoma tumour. After oral administration of radiolabeled gold nanoparticles to rabbit, it was found that the particles were released to colon due to solubility of hemicelluloses in basic media suggesting that they can be used for targeted delivery at colons. In serum proteins the gold nanoparticles bound preferentially to globulins with the order; α1-globulin (6.5%) followed by α2-globulin (~2%), β-globulin (~1%), γ-globulin (0.707%) and albumin (0.66%).
حدود آرڈیننس 5 جولائی 1977ء کو ضیا ء الحق نے وطن عزیز میں مارشل لا ء نافذ کیا۔یہ حقیقت ہے کہ اس مارشل لاء کو عوامی حمایت حاصل تھی۔ ضیاء دور میں اسلامائزیشن کےلیےبہت ٹھوس اقدامات کیے گئے۔ اس عہد کا ایک بڑا کارنامہ اسلامیہ جمہوریہ پاکستان میں حدود قوانین کا نفاذ ہے جواسلامی نظریاتی کونسل نے تقریباً ڈیڑھ سال کی جہد مسلسل کے بعد پانچ مسودات کی صورت میں پیش کیا۔ ان مسودات پر عمل درآمد کا مقصدبراہ راست معاشرے کی اخلاقی اقدار کے تحفظ اور جائیداد کی حفاظت سے تھا،یعنی ان کی تنفیذ سے مقاصد شریعت کا تحفظ ممکن تھا۔ ان سفارشات کے ذریعے چوری ، ڈاکہ ، زنا، قذف اور شراب نوشی وغیرہ جرائم سے متعلق مروجہ قوانین کو بدل کر اسلامی قوانین کا نفاذ کر دیا گیا اور ان جرائم کے ارتکاب پر قرآن وسنت کی مقرر کردہ سزائین نافذ کر دی گئیں ۔ 12 ریبع الاول 1399 ھ کو رسول اللہ ﷺ کی ولادت با سعادت کے دن بمطابق مورخہ 10 فروری 1979 ء کو ضیاء الحق نے ایک صدارتی حکم نامہ کے ذریعے مند رجہ ذیل حدود قوانین کا اجراء کیا: 1. جائیداد (منقولہ )سے متعلق جرائم(نفاذ حدود) آرڈیننس 1979ء Offences against Property (Enforcement of Hudood) Ordinance, 1979, VI of 1979. اس قانون کو نافذ کرنے کا بنیادی مقصد جائیداد (منقولہ) سے متعلق ہونے والے کچھ خاص جرائم کو اسلامی شریعت کے مطابق ڈھالنا ہے اور اس سے مال کی حفاظت مقصود ہے ۔ یہ قوانین چوری یا ڈکیتی وغیرہ کے زمرے میں آتے ہیں۔ اس آرڈیننس کے تحت کل چھبیس دفعات ہیں، پہلی دفعہ یہ ظاہر کرتی ہے کہ یہ قانون 10 فروری 1979ء بمطابق 12 ربیع الاول 1399 ہجری سے پورے پاکستان میں فافذالعمل ہوگا ۔ 422 دوسری دفعہ مختلف تعریفات پر مشتمل ہے جو اس قانون...
Civic behavior reflects the values related to individual’s attitude towards family, peer group, and neighborhood and includes the way one behaves in the society and community as a whole. The purpose of the present study is to describe what civic behavior is, its characteristics, its status in the Hadith, and the way forward for improvement of the civic behavior as guided by Sīrah of the Holy Prophet (ﷺ). Study implies that truthfulness, mercifulness, polite talk, helping others, respect for others, caring and sharing and promise-keeping, loyalty, sympathy, honesty, kindness, brotherhood, cooperation, fairness and unity are the core qualities that make the individual a civilized and active citizen and our Holy Prophet (ﷺ) is the embodiment of all these virtues. Descriptive approach was followed to explain and analyze the concept. First the concept of civic behavior was interpreted, then its importance and characteristics were discussed. After that it was explored in the light of the teachings of the Quran and the Prophet Muhammad (ﷺ). The findings highlight that the civic behavior in its best form was preached and practiced by the Holy Prophet (ﷺ) and his companions. Each dimension was interpreted in the light of the Quran and the Sīrah. It was concluded that Sīrah of the Holy Prophet (ﷺ) is an immaculate source of guidance and inspiration for not only Muslims but also for the whole mankind in acquiring the core values and abilities that determine the Civic behavior of an individual which ultimately results into a well-organized, peaceful and civilized society The study suggests that civic behavior of the citizens has significant impact on the formation and development of a peaceful society. It is recommended that civic behavior as reflected in the life of and practiced by the Holy Prophet (ﷺ) should be promoted and practiced for achieving a civilized social setup.
The main argument of this study is that the issue of Kashmir can be resolved through the third party intervention. It is also argued in the study that the resolution of issue needs to be resolved in order to avoid Nuclearization in South Asian region. The study concludes key arguments about Kashmir. This dissertation is divided into seven chapters. The first chapter articulates the fact that the legal position of these states was that they could join India, Pakistan or remain independent. However, Kashmir was forcibly occupied by India. Illegal annexation of Kashmir by India not only undermined the legal statutes of the state of Kashmir but also violated main principles of the Partition Plan. It is stated that according to the plan the areas joining India or Pakistan were to be considered on the basis of geographical nearness and cultural similarity. These factors were in the favor of Kashmir’s annexation with Pakistan which was not acceptable to India. Thus, India engaged Kashmir without considering the legal aspects of the partition. This situation developed the dispute between India and Pakistan. United Nations have tried passed several resolutions but the same were not implemented by India. It is concluded that conflict of Kashmir was an outcome of unfair Partition Plan. The second Chapter is about research methodology. It gives consolidated literature review which is focused on main concepts and key arguments. This chapter also includes objectives and research questions of the study followed by justification. The third chapter of thesis engages the readers in understanding moral and legal aspects of Kashmir. This chapter uses the legal documents to demonstrate that the argument concluded between Maharaja and the British Government was based on certain terms and conditions which have been described under various articles of the treaty. In addition, the chapter also deals with standstill agreement of Jammu and Kashmir with Pakistan. Pakistan responded to the agreement indicates that both parties accepted the argument. However, it can also be noted that there was no such settlement concluded between Jammu and Kashmir and India. The second part of the chapter deals with various point of views which can be interpreted as Indian standpoint, Pakistan perception and Kashmiri people’s views. These perspectives can be used to understand the issue of Kashmir and to solve it amicably. However, the conflict among the various stakeholders could lead to increase the nuclear tension and could convert this problem into nuclear clash. The third part of the chapter concludes that the process of nuclear proliferation was an outcome of unresolved issue of Kashmir, which is a major concern of international community. The fourth chapter focuses on the United Nations initiatives, taken by the international organization since, 1948, the complete resolutions, the report of commissions, efforts of different legal, political, international law experts to resolve the dispute between India & Pakistan, on the issue of Kashmir. The chapter also highlights the positive outcomes of international community specially, United States of America, United Kingdom and others as third party facilitators to resolve this issue properly. The chapter reflects the clear picture about the parties involved and their attitude to resolve this issue and defines the stumbling block, deadlock created by one party to the other. The chapter will further facilitate researchers, scholars and governments of India and Pakistan to initiate new level of dialogue to resolve this core issue of South Asia for the peace and stability of the region. The fifth chapter concludes the major events of history, past, present and future developments related to the dialogue process between the leaders of Pakistan and India on the Kashmir issue, if we remind the series of negotiated settlements since 1947 and onwards. The chapter has examined various factors for partition and concluded that joining of the people of Kashmir. After partition, the talks between newly established countries of Pakistan and India began on the question of Kashmir issue. The political leadership of India always showed myopic vision and refused to accept any logic to the just and fair solution of the State of Jammu and Kashmir, so she used delaying tactics and aggressive, illogical attitude towards Pakistani leadership and the people of Jammu and Kashmir State. Thus, it is argued in this chapter, that there is a need to change the attitude of Indian leadership. It is further argued that Indian leaders should not undermine the moral authority of United Nations. They should respect United Nations Security Council Resolutions and facilitations offered by International community and friendly nations. There is a need of displaying positive political vision, flexibility and accommodative thinking. The sixth chapter is based on the exploration of various proposals to form new recommendations for the solution of Kashmir problem. This chapter also justifies the need to initiate dialogue process between two countries and it also justifies the need for third party involvement. The United Nations, international super powers, like United States of America, Russia, United Kingdom and friendly countries need to play an effective role in the resolution of Kashmir issue. The chapter also has also investigated the theories of resolution in the international context expressed by Jagat Mehta, B. G. Verghese proposal, the Galbraith plan (Harvard Exercise) which proposed opening of road across the cease- fire line between Rawalpindi and Srinagar. These theories can also help to reduce the intensity of conflict. In addition, the conclusion draws attention of political leadership of both India and Pakistan to show flexibility and political acumen, intellectual wisdom and broad mindedness to address and resolve the issue of Kashmir. The seventh chapter is focused on the conclusion and recommendations about the issue of Kashmir.