EFFECTS OF PHYSICAL THERAPY-BASED MANAGEMENT APPROACHES FOR TENSION TYPE HEADACHE

Background of the Study: Multiple Physical Therapy approaches have recently been developed and reported in the literature for providing better results in the treatment regimens of tension-type headaches. The advancement in the field of Physical therapy towards the treatment approaches of tension-type headaches has become the driving force for writing this article. Methodology: Studies comparing the effects of physical therapy management with conventional treatment approaches are included in the meta-analysis. PRISMA guidelines were used for performing the qualitative analysis and assessment of risk of biases. Results: According to the findings of nine randomized controlled trials, the analysis of the results had revealed that physical therapy intervention demonstrated a significant improvement in reducing headache severity. In a random effect model, the pool effects of physical therapy strategies in terms of Standardized Mean Difference had an impact of 1.41, which according to a Cohen rule of thumb displays a larger effect of physical therapy management in significant decrease in pain intensity among tension-type headache patients Conclusion: The study has concluded that physical therapy-based management strategies as provided in several RCTs analyzed in this review article revealed a pool effect of moderate size in managing the frequency of pain and a larger effect size in managing pain intensity and duration. Further, it was concluded that tension-type headaches can be effectively managed through physical therapy-based approaches.

Development and Optimization of Polymerase Chain Reaction for Screening of Burkholderia Mallei Infection Among Indiginous Working Equines

Economically, Pakistan is an agricultural country as the agriculture sector offers employment to 42.3% of the country?s citizens involved with any kind of labor for their livelihood, 19.5% of its gross domestic product (GDP) comes from the agriculture sector apart from the raw materials for many other value-added sectors contributing to huge quantities of added indirect income; livestock sector as for instance, adding 58.33% to the worth of agriculture besides contributing 11.39% directly to GDP. Population of livestock are cattle 44.4, Buffalo 37.7, Sheep 30.1, goat 72.2, Camel 1.1, Horses 0.4, Asses 5.2, Mules 0.2 millions. Asses population increased from 4.9 to 5.2 million while population of Camel, Horses and mules remained unchanged since last year. Horses along with mules and donkeys facing many managerial, nutritional, noninfectious and infectious diseases which decreases production and performance, out of which infectious diseases such as Glanders is life threatening and pose zoonotic potential to human beings specially care takers, veterinarian and public health. To monitor threat due to these asymptomatic carriers to animals and human beings and from places having decreases frequency of disease Glanders in equines, it is unavoidable to develop and optimize a highly specific and sensitive molecular test such as PCR, required to decrease the number of results which are false negative and false positive findings besides being fast and accurate. 200 nasal and blood samples of working equines (horses, mules and donkeys) were collected from the Bahawalpur, Faisalabad, Gujranwala, Lahore and Multan at the sites of Brook hospital. All the samples were collected and transported to the WTO Quality Control Laboratory of UVAS, Lahore keeping in account the all microbiological hygienic and hazardous measures according to OIE protocols. All the animals were injected mallein PPD intra-dermally into the lower eyelid with the result noted after first and second day. Blood of animals were used for DNA extraction by commercially available DNA extraction kit. The extracted DNA were subjected to Gel Electrophoresis for integrity of the extracted DNA and then used further for amplification of target region of the DNA. The extracted DNA was used for the amplification of Flip IS407A gene region using the specific primers. Products of the PCR were visualized with UV light after electrophoresis of the gel and B. mallei was detected in only positive control. The study found high prevalence of the disease and existence of active nasal shedders in equines. The findings implicate importance of targeted surveillance in glanders suspected equines.