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Identification and Functional Characterization of P2- Type Calcium Atpases in Selected Species of Gramineae

Thesis Info

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Author

Roohi. Aslam

Program

PhD

Institute

National University of Sciences & Technology

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9486/1/Roohi_Aslam_Viorlogy_%26_Immunology_HSR_2018_NUST_01.08.2018.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726389324

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Calcium and manganese perform various important biological functions within the plant body, including signal transduction, proper cell wall formation, photosynthesis, respiration and nitrogen assimilation etc. Calcium and manganese exist in the form of ions within cells. P2- type calcium ATPases are responsible for maintaining homeostasis of these ions in both domains of life. P2- type ATPases are further divided into P2A (ECAs) and P2B- types (ACAs). In present research work, BLAST searches in different databases were conducted for the retrieval of calcium ATPase sequences of selected monocots and a phylogenetic analysis was performed. Furthermore, Triticum aestivum plants were grown under calcium and manganese stress using hydroponic culture. The tissue generated was used to find out the expression of selected P2A and P2B- type calcium ATPases under manganese and calcium stress. Moreover, an ECA3 construct was generated and it was transformed into yeast model (Pmr1) to find out the possible role of this gene in manganese nutrition. Overall, phylogenetic analysis revealed a high degree of evolutionary relatedness among P2- type calcium ATPases. High expression of selected ACAs was observed in T. aestivum plants grown under calcium stress, which gave clues to the role of ACAs in signal transduction in T. aestivum. High expression of selected T. aestivum ECAs under calcium and manganese stress was also observed which may indicate the potential roles of these ATPases in calcium and manganese nutrition in T. aestivum. Additionally, ECA3 gene (which is a P2A- type ATPase) was amplified using T. aestivum cDNA and sequenced subsequently. The data indicated that it retained introns in some of the transcripts. The qRT-PCR analysis revealed that events of intron retention in ECA3 transcripts were much enhanced when plants were exposed to calcium stress. ECA3 construct was further transformed into yeast mutant Pmr1, which is a null mutant for xi its own manganese pump and cannot grow on high manganese concentration in the medium. Interestingly, ECA3 rescued the phenotype and helped the mutant to grow successfully on manganese toxic media. This further indicated, the importance of ECA3 in manganese translocation in T. aestivum. Hence, ECA3 gene is reported here as first P2A- type pump identified in T. aestivum and is required for manganese management in this species. its own manganese pump and cannot grow on high manganese concentration in the medium. Interestingly, ECA3 rescued the phenotype and helped the mutant to grow successfully on manganese toxic media. This further indicated, the importance of ECA3 in manganese translocation in T. aestivum. Hence, ECA3 gene is reported here as first P2A- type pump identified in T. aestivum and is required for manganese management in this species.
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مولوی حسین حسان ندوی

مولوی حسین حسان ندوی
راقم کے لیے تیسرا حادثہ طالب علمی کے زمانہ کے رفیق مولوی حسین حسان صاحب ندوی اڈیٹر پیام تعلیم کی وفات کا ہے، مرحوم درجہ میں مجھ سے دو تین سال نیچے اور غالباً عمر میں بھی اسی قدر چھوٹے تھے، لیکن ہم دونوں عرصہ تک ایک ہی کمرے میں رہے تھے، اس لئے ان سے دوستانہ تعلقات تھے جو آخر تک قائم رہے، اسی زمانہ سے ان میں مضمون نگاری کا ذوق تھا، چنانچہ عشاق عرب کے عنوان سے ایک طویل مضمون لکھا تھا۔ جو زمانہ کانپور کے کئی نمبروں میں چھپا تھا، ندوہ سے فراغت کے بعد جامعہ ملیہ چلے گئے اور وہاں تعلیم کے ساتھ مختلف اوقات میں جامعہ کے مختلف شعبوں سے ان کا تعلق رہا، بچوں کا ادب لکھنے میں ان کو خاص ملکہ تھا اس کے وہ صاحب طرز ادیب تھے، برسوں بچوں کے رسالہ پیام تعلیم کے اڈیٹر رہے اور اس کو ان کا بڑا مقبول رسالہ بنادیا، پیام تعلیم کے مضامین کے علاوہ انھوں نے بچوں کے ذوق کی بہت سی کتابیں لکھیں اور بچکانہ ادب کا بڑا ذخیرہ فراہم کردیا، ان سے تعلقات کا سلسلہ برابر قائم رہا، کبھی کبھی ملاقات بھی ہوجاتی تھی، گزشتہ دسمبر میں دلی میں ملاقات ہوئی تھی، کیا معلوم تھا کہ یہ آخری ملاقات ہے، اﷲ تعالیٰ ان کی مغفرت فرمائے۔ (شاہ معین الدین ندوی، اگست ۱۹۷۴ء)

 

قانون ٹارٹ كا فقہ اسلامى كى روشنى میں جائزہ

Tort law is an umbrella term for  laws  which cover issues of civil wrongs like defamation, trespassing and the other actions involving  violation of law. In case a person has undergone a physical, legal or any economic harm then he can file a suit under the tort law. Torts are civil wrongs recognized by law as grounds for a lawsuit. It is also generally known that tort in Islamic fiqh as “Jinayet”. This paper attempts to analys  by Islamic law in thel light of the  relevant verses for the Qur’an followed by the rules stated in traditions from the Prophet (Peace by on him). Jinayat the part of Shari’a that applies to homicide or physical  injury is called jinayat and is based on the pre-Islamic rules of Arab blood feud, as modified by Prophet (Peace be on him). The punishment is either retaliation or blood money (diyat). Retaliation occurs only upon  the request of the victim, if alive, or his nearest kin if the victim is dead, and is to be inflicted by victim or kin. In the case of homicide retaliation means death, in the case of injury it means imposing an identical injury. Where retaliation is one of the options, the victim or his closest kinsman may demand blood money instead, or negotiate an out of court settlement. Jinayat, like modern tort law, is based on private action; there is no official responsible for initiating the case.

Study of Effective Virtual Project Management Through Emotional Intelligence, Empowerment and Leadership Style in Partly and Truly Global Projects

Increasing globalization of organizations and proliferation of new technologies have made multi-cultural virtual teams not only a reality but important also. Global virtual team members rarely meet face-to-face and thus deal with challenges which not associated with traditional collocated teams (Lipnack & Stamps, 1997). These challenges faced by virtual project team members form the basis for the first phase of research. The research was conducted in three phases. Initially, in phase one of the research, important behavioral contributors to virtual project management were identified from the literature. The researcher gathered these contributing factors within a framework named the “Factor Reinforcing Model®”. This model was empirically tested through statistical method, against data on the relevant variables, obtained from a survey of fourteen software houses in Pakistan. Findings from the model conclude that several contributing factors of virtual project management (VPM), namely, communication, motivation, information security, trust building etc reinforce each other to lead to effective virtual project management. In the second phase of the thesis, the researcher conceptualized that Emotional Intelligence (EI) plays a very decisive role in the performance of managers even in a VPM environment. She tested the proposition that there is an overlapping relationship between the factors contributing to effective virtual project management and the competencies of emotional intelligence. Findings highlight that emotional intelligence together with soft skills and intelligence help in uplifting motivation, reducing cultural barriers, resolving conflict, building teamwork and collaboration and enhancing communication among professional project team members. Thus research findings establish that EI helps project managers to cope with the challenges of VPM through self-awareness, self-management, social awareness, and relationship management. In phase three of the research, the researcher analyzed the preferred leadership behaviour for effective project management. Leadership behavior is measured by concern for tasks and ivconcern for people in partly and truly global projects. Factors based on leadership behaviour were identified like participative decision making, open communication, conflict management, delegation of power, task monitoring, time management, coaching, and team work and a model for effective project management was proposed in leadership context. In addition to above, in the third phase, the relationship of social intelligence and leadership style in partly and truly global virtual projects was also analyzed. Results showed that social awareness and relationship management were positively related to concern for task and concern for people and was higher in truly global than partly global virtual projects. A model of effective virtual project management was proposed from research findings regarding SI. The relationship between empowerment climate and leadership style and customer service as a measure of effective project management was also analyzed in projects with varying degree of virtuality. Initially the researcher compared the empowerment environment among more and less virtual projects. Then she examined the moderating effects of degree of virtuality on the relationship between empowerment climate and leadership style. The findings of this research suggest that high empowerment climate and concern for people are highly demanded by truly virtual projects than partly virtual projects. A model of effective virtual project management was proposed with respect to empowerment climate, leadership style (concern for people) and customer service. Moreover, it was found that virtuality moderates the relationship between empowerment climate and concern for people. The findings of this research have significant implications for leadership skills and behaviours at partly global and truly global project management workplaces.