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Identification of Biomass-Related Qtls in Sorghum Sorghum Bicolor L. Moench Using Association Mapping

Thesis Info

Access Option

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Author

Sarmad Frogh Arshad

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/8448/1/Sarmad_Frogh_Arshad_Biotechnology_HSR_2017_UAF_22.11.2017.pdf

Added

2021-02-17 19:49:13

Modified

2023-02-17 21:08:06

ARI ID

1676726392993

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یہ ناداں گر گئے سجدے میںجب وقت قیام آیا

یہ ناداں گر گئے سجدے میں جب وقت قیام آیا
نحمدہ ونصلی علی رسولہ الکریم امّا بعد فاعوذ بااللہ من الشیطن الرجیم
بسم اللہ الرحمن الرحیم
معزز اسا تذہ کرام اور میرے ہم مکتب ساتھیو!آج مجھے جس موضوع پر اظہار خیال کرنا ہے وہ ہے: ’’یہ ناداں گر گئے سجدے میں جب وقت قیام آیا‘‘
صدرِذی وقار!
ہر کام کا وقت مقرر ہے ، چمنستان میں گل نرگس کی شگفتگی کا ایک وقت ہے، نکہت باد بہاری کا ایک وقت ہے، کھیت اورکھلیان کے کشتِ زعفران بننے کا وقت ہے، طائر غور وفکر کی پرواز کا ایک وقت ہے، طفلانِ خود معاملہ کے عالم شباب میں پہنچنے کا ایک وقت ہے۔
صدرِمحترم!
انسان اشرف المخلوقات ہے ،شرف و بزرگی کا تاج اس کے سر سجا ہوا ہے، مجدی وسروری کی خلعتِ فاخرہ اس نے زیب تن کی ہوئی ہے ،عقل وشعور کی دولت سے مالا مال ہے ، مسجود الملائکہ کے خطاب سے نوازا گیا ہے۔ لیکن اسے بھی مسند رفیعہ کا صد رِنشیں بننے میں ایک وقت درکار ہے اس کوبھی منازل اپنے وقت پر ملتی ہیں۔
جنابِ صدر!
کام کو وقت پر کرنا ایک دانشمندی ہے، کمرۂ تدریس میں کتاب پر بھر پور توجہ عقلمندی ہے، مجاہد کی نظریاتی و جغرافیائی سرحدوں کی حفاظت دانشمندی ہے۔ خطیب کی اپنے خطبے کے لیے مکمل تیاری دانائی ہے، تاجر کی تجارت میں مکمل شمولیت عقلمندی ہے، زمستانی ہوائوں میں گرم لباس کا استعمال دانشمندی ہے، موسم گرما کی مسموم ہواؤں میں ہلکا پھلکا لباس استعمال کر نادانائی ہے۔
صدرِمحترم!
اگر کوئی جتنا بھی فطین ہے، ذہانت و فطانت کے میدان کا شاہسوار ہے، عقل وخرداس کے گھر کی لونڈی ہے، شعور وآگہی کی فاختاؤں نے اس کے گھر میں موجو دشجر سایہ دار پر اپنا آشیانہ بنا رکھا ہو، اس کی عقل...

جدید تفسیری ادب میں بنت الشاطی کی امتیازی خدمات

Āisha bint Abdu Al-Rahmān (1913-1998), better known by her pen nickname ‘Bint Ash-Shātī’, was one of the 20th-century Egyptian exegetes (mufassirīn) of the Qur'ān who implemented the methodological approach to the interpretation of the Qur'ān introduced by her teacher, mentor and husband Amīn alKhawlī, an eminent Egyptian scholar of the Qur'ān who contributed to the Qur'ānic sciences from several important aspects. Bint Ash-Shātī continued the legacy of her teacher and published a number of works related to the discipline of the Qur'ānic sciences such as al-Tafsīr al-Bayānī li al-Qur'ān alKarīm and al-I’jāz al- Bayānī li al- Qur'ān wa Masā’īl Ibn alAzraq. She made an attempt to examine all the previous Qur'ānic exegeses. Though Bint Ash-Shātī benefited from the classical tafsīr literature in her writings, she made several critiques on the classical tafsīr literature. Because of her scholarly approach, she became a famous Qur'ānic scholar in her life and even she was awarded the King Faisal Award,  the most prestigious award in the Muslim world, in 1993 for her intellectual contributions. This paper attempts to analyze the methodological approach adopted by Bint Ash-Shātī in her works on Qur'ānic studies.

Characterization of Ligninolytic Microbial Consortia and Analysis of Recalcitrant Structural Properties of Pretreated Lignocellulosic Biomass

The aims of this dissertation was to evaluate chemical and biological treatment methods to remove and degrade lignin from agriculture waste biomass for increasing the yield of biogas and biohydrogen. In chemical treatment approach, three alkali reagents at various dosages: NaOH (1-5%), KOH (1-5%), and Ca(OH)2 (0.5%) and three different heating processes, water bath, autoclave and short time microwave were tested for 10 different agriculture substrate. The Scanning Electron Microscopy (SEM) images showed visible degradation on the alkalies treated surface of biomass as compared to the untreated biomass. Additionally, disapperance and emergence of new peaks were observed in treated substrates using Fourier Transform Infrared spectroscopy (FT-IR). Microwave heating with 2% NaOH treated substrates showed more total biogas yield as compared to other treatment conditions. The Ca(OH)2 (0.5%) soaking of corn cob for 7, 15, and 30 days incubation was tested. The highest cumulative biogas was 360.5 NmL/gVS, 3-times higher than the cumulative biogas produced from the untreated corn cob 115.1 NmL/gVS. For biological treatment of waste material, 27 ligninolytic bacteria were isolated from soil, wood compost, and waste sludge. Seven of the most active strains were selected. The optimum yields of lignin peroxidase and laccase were achieved at pH 3-5. The co-cultures demonstrated 2.5 times more rice straw lignin degradation than using single culture. Likewise, the greatest enhancements of cumulative methane yield (70-76%) occurred from co-cultures treated rice straw as compared to individual culture. To produce biohydrogen and biomethane separatly in batch fermentation, 20 ligninolytic Bacillus sp. strains were isolated from granular sludge of full scale anaerobic digester. Among them, four ligninolytic Bacillus sp. strains were selected based on their lignin and Azure B degradation. Brevibacillus agri AN-3 exhibited the highest decrease in COD (88.4%) of lignin and (78.1%) of Azure B. Brevibacillus agri AN-3 showed hydrogen (H2) yield of 1.34 and 2.9 mol-H2/mol from xylose and cellulose respectively. In two-phase wheat straw batch fermentation, Brevibacillus agri AN-3 produced 72.5 and 125.5 NmL/gVS cumulative H2 and methane (CH4) respectively. It was perceived that using ligninolytic Bacillus sp. strains, 48.6% more methane yield could be obtained xx from the wheat straw than using the untreated wheat straw in batch fermentation. A consolidating bioprocessing of recombinantecombinantecombinant ecombinant ecombinant NeurosporaNeurosporaNeurospora NeurosporaNeurospora crassa F5 strain was used for saccharification of wheat straw (WS) to increase the biogas production. The WS was pretreated with 2% NaOH followed by 2,4, and 6 days hydrolysis with N. cN. c N. crassa F5 strain at 28±1℃ and 200 rpm using 0.5 g/L glucose in Vogel media. Scanning Electron Microscopy (SEM) analysis showed a visible change on the surface structure of the pretreated WS as compared to the untreated WS. The 2,4 and 6 days N. crassa F5 saccharified WS was used for biomethane potential (BMP) analysis using automatic methane potential testing system (AMPTS). A maximum cumulative biogas of 700.8 mL/gVS was obtained from 2% NaOH pretreated WS followed by 2 days N. crassa F5 treatment. The recombinant ecombinant ecombinant ecombinant ecombinant N. cN. c N. crassa F5 treated WS produced daily biogas which was 6-fold higher per day and 339.3% more in cumulative volume than the untreated WS sample. Finally, a single culture was tested for the potential of biohydrogen from Organic Fraction of Municipal Solid Waste (OFMSW). One hundred and twenty bacterial strains were isolated from heat-treated granular sludge of a full scale anaerobic digester. The best hydrolytic strains were assessed for H2 production from glucose and soluble starch. Two Bacillus sp. strains, namely F2.5 and F2.8, exhibited high H2 yields and were used as pure culture to convert OFMSW into hydrogen. The strains produced up to 61 mL of H2 per grams of volatile solids and could be considered as good candidates towards the development of industrially relevant H2-producing inoculants. This was the first successful application of pure microbial cultures in bio-hydrogen production from OFMSW.