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Identification of Differetially Expressed Proteins in Oral Squamous Cell Carcinoma Oscc Through Proteomic Analysis

Thesis Info

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Author

Urooj, Uzma

Program

PhD

Institute

University of Karachi

City

Karachi

Province

Sindh

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/11744/1/Uzma%20Urooj%20Biochemistry%202017%20uok%20karachi%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726394037

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Oral squamous cell carcinoma (OSCC) is the most common malignancy in head and neck region with an occurrence rate of 500,000 per year. Higher incidence and mortality rates have been observed in south-east Asia including Pakistan. The common sub-site found in Pakistani population and other physiological variations are strongly linked with the increasing use of smokeless tobaccos (SLTs). Early diagnosis plays a key role in treatment and monitoring of any cancer. Histopathology is the only diagnostic method recommended for OSCC which is not very effective for early stage diagnosis. Thus, biomarker studies have been conducted all around the world using proteomics techniques in search of potential candidate diagnostic biomarkers. In current study, we have performed comparative proteomic analysis using buccal mucosa OSCC tissue samples and their adjacent normal mucosa. The samples were subjected to two-dimensional gel electrophoresis and differentially expressed spots were selected for Mass spectrometric analysis. We have identified seventeen proteins that were consistently differentially expressed among most of the samples analyzed. The differentially expressed proteins that have been uniquely identified in this study include Serum albumin (ALBU), Ig Kappa chain C region (IGKC), and Isoform 2 of fructose bisphosphate aldolase A (ALDOA). We suggest that further research would be required to investigate their role in carcinogenesis and whether any of these could serve as potential biomarker. The significantly altered expression of Calreticulin (CALR), Heat shock protein beta 1 (HSPB1), Heat shock protein 70 (HSC70), Cytokeratin-8 (KRT8), Cytokeratin-16 (KRT16), Cytokeratin-14 (KRT14), Cytokeratin-17 (KRT17), and 14-3-3σ/Stratifin (SFN) found in our study strongly supported the earlier reported results. We also performed immunohistochemistry (IHC) to confirm the higher expression of SFN as potential biomarker. SFN plays an important role in p53 apoptotic regulation and therefore findings of our study reveal and suggest it as a potential candidate biomarker in Pakistani population. XII Considering the association of oxidative stress with cancer progression, we also studied markers of oxidative stress in OSCC including Paraoxonase1 (PON1), Reactive oxygen species (ROS), and Malondialdehyde (MDA). We found significant relationship among oxidative stress indicators, particularly PON1 with OSCC suggesting PON1 as predictive marker.
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۔حکیم محمد صادق سیالکوٹی ؒکی اردو سیرت ’’جمالِ مصطفیؐ‘‘کا تعارف وجائزہ

حکیم محمد صادق سیالکوٹی ؒکی اردو سیرت ’’جمالِ مصطفی‘‘کا تعارف وجائزہ

ڈاکٹر محمد انصر جاوید گھمن

 اردو سیرت  کی کتاب "جمالِ مصطفیﷺ"مولاناحکیم محمدصاد ق سیالکوٹی رحمہ اللہ کی ایک مشہور تصنیف ہے ۔ یہ کتاب نعمانی کتب خانہ حق سٹریٹ اردوبازار، لاہورسے چھپی ہے ۔اس کتاب کا  سرورق بہت زیادہ خوبصورتی سے مزین کیاگیاہے، جس کی ابتدااللہ تعالیٰ کے ارشادِ پاک "اور ہم نے آپﷺ کو سب جہانوں کے لیے رحمت بناکربھیجاہے۔"کے ساتھ درج ذیل شعر سے کی گئی ہے:

سیلاب رنگ و نور طلوع سحر میں ہے                تابندہ کہکشاں تیر گر سفر میں ہے

 حکیم صاحب اپنی کتاب کا تعارف  ان الفاظ میں کرواتے ہیں:

"اس کتاب میں آرامِ جاں، سکون ایمان، کاشفِ سرکن فکان، دلدارِ مسکان، غم خوارِ عاصیاں، ممدوحِ قُدْسَیاں، سرخیلِ نوریاں، رحمتِ عالمیاں، سید الکونین، سید الثقلین، حبیبِ خدا، اشرف الانبیاء، شافعِ روزِ جزاء، حضرت محمد مصطفیﷺکا حسن صحیح معنوں میں اپنی راعنائیوں کے ساتھ جلوہ بار ہے۔"

 مولانا حکیم محمد صادق سیالکوٹی رحمہ اللہ نے حسنِ صورت بھی بیان کیاہے اور حسنِ سیرت بھی بیان کیاہے۔ حکیم صاحب نے مندرجہ بالا القابات جو بیان کیے ہیں در حقیقت یہ رسول اللہﷺ کی سیرت کے مختلف پہلو ہیں جو حکیم صاحب نے بڑے حکیمانہ انداز میں بیان فرمائے ہیں۔ پھرحکیم صاحب نبیﷺ کی سیرت کو اشعارکی صورت میں بیان کرتےہیں اور آفتابِ نبوت میں آفتابِ کردار کو نمایاں کرتے ہیں۔جن میں سے چند حسب ذیل ہیں:

نگاہ برق نہیں چہرہ آفتاب نہیں

وہ آدمی ہے مگر دیکھنے کی تاب نہیں

کہکشاں ہے تیرے اہرار مقدس کا غبار

تیرے نقش پا ہیں فردوس بریں کے لالہ زار

 حکیم صاحب...

Momentum of Ajwa Dates towards Cardiovascular Diseases Momentum of Ajwa Dates towards Cardiovascular Diseases

Cardiovascular diseases (CVDs) are the top most cause of morality around the world.  It is predicted that the number casualties from CVDs will increase to more than 24 million till 2030 people. Medicinal plants provide the major raw materials for medicine preparations. They are gaining high consideration due to their effectiveness and increasing cost of modern medicines. Many successful drugs are plant based, including aspirin from the willow bark, morphine from opium poppy, quinine from the cinchona bark, and digoxin from the foxglove. According to World Health Organization (WTO), ~70% to 80% of people around the world rely on herbal sources for the treatment of their disease. Plant sources are endorsed due to the fact that they contain an optimal amount of  antioxidants and phytochemicals that help to avoid and treat many diseases. Phoenix dactylifera L. Particularly Ajwa variety, is the most rich in phytonutrientsthat can benefit to control many cardiovascular diseases. It contains6 vitamins (vitamin A, C, B1, B2, B3 &riboflavin), high amount of fibers, Potassium, Magnesium and 23 amino acids which play a healthy role towards hypertension, muscular contractions, and blood pressure control. It has been studied that Niacin (B3) helps to control cholesterol and low density lipoprotein levels (LDL), as high cholesterol is the one of the main cause of cardiovascular diseases so, Ajwa could be a vital regulatory source. According to the findings of Sabbah M. Et al, Ajwa extracts significantly improved the DNA integrity and also reduced the cardiomyocytes congestion, edema and the cellular stress wielded on cardiac muscles resulting the restoration of cardiomyocytes architecture in Doxorubicin (DOX) induced cardiotoxicity in rats. Research done by Alqarni et al, proves that Ajwa extracts has successfully decreased the LDL‐C, VLDL‐C, and triglycerides concentration. Additionally, treatment with ajwa pulp also improved the HDL‐C level and antioxidant enzymes activity. In another invivo study, Ajwa preparation has successfullydecreased the diclofenac-induced pulmonary and hepatic instabilities. Vitamin-K play important role in blood coagulation, and in case of anticoagulant therapy, activity of vitamin-K controlled by drugs (warfarin) that sometimes causes serious side effects. According to the reported data, Salicylic acid is the vitamin-K antagonist and has capability to block the action of vitamin K during the coagulation pathway. Dates contain ~3.75 to 4.50 mg/100 g of salicylic acid. Thus, providing anticoagulation effect too. So, the limelight of the reported data provides an enough reason that plants can be used as primary source of drug designing for the cardiovascular disease. They hold true momentum to address the increasing healthdiseases, which cannot be lost to distraction or apathy. Fight against the burden of CDVs, is affecting all countries and specially, under developing and the poor countries.

The Effects of Computer Assisted Instructions on the Academic Achievement, Interest, and Retention of the Students in Two Secondary Schools in Dera Ismail Khan, Pakistan: An Experimental Study

Title: “The effects of Computer Assisted Instructions on the academic achievement, interest, and retention of the students in two secondary schools in Dera Ismail Khan, Pakistan: an experimental study” Researcher: Liaquat Hussain Supervisor: Dr. Umar Ali Khan University: Gomal University, Dera Ismail Khan, KPK, Pakistan Year: 2012 Subject Area: Education Degree: Ph.D. There are limited domestic studies on the effect of Computer Assisted Instructions (CAI) that influences on academic achievement. The only one of research that found is on retention. Those studies are also not on the subject of physics and do not cover the demographic effects. The present study was an experimental study with its objective to examine the effects of Computer Assisted Instructions on the academic achievement, interest, and retention of the students in two secondary schools in Dera Ismail Khan, Pakistan. Methodology: The experiment was performed on total 146 (Male= 66 & Female=80) students at the secondary level who were enrolled in academic year 2012. The whole sample was equated into two groups on the basis of pre-test. One group was randomly assigned as the experimental group and the other as Control group. The experimental group was taught by the Computer Assisted Instruction (CAI) method and the control group was taught by the traditional lecture method. After one month treatment both the groups were given the post-test and interest inventory, and delayed post-test was administered two months after post-test. vi Data were analyzed by using the Mean, Standard Deviation, t-statistics, ANOVA and Regression analysis as statistical techniques. Findings and Conclusion: CAI method of instruction was found more effective as compared to Traditional Lecture method in enhancing the academic achievement, interest and retention of students in physics in two secondary schools. When the gender differences were analyzed CAI was found more effective for both male and female as compared to Traditional lecture method yet there were gender differences within the group. In overall female were better than male on achievement in both CAI and lecture methods while male were better than female in interest in both CAI and lecture method. The retention score of male and female shows that female were better in retention in lecture method while there was no difference in retention of male and female in CAI method. Though CAI is more effective for both Rural and Urban as compared to Traditional lecture method yet there were no Location differences within the groups. Also CAI is more effective for both students having a low or a high father income level as compared to Traditional lecture method. There were also no difference of father educational level (Graduate, under-graduate, and illiterate) and the father''s income (greater than 10000, 10000) on academic achievement, interest and retention within the experimental group taught through CAI method. Yet there have been father income and educational level differences in control group taught through lecture method. Similarly CAI is most effective for students having different languages at home (Urdu, Saraiki, and Pashto) as compared to Traditional lecture method yet there were Language level differences. In the experimental group taught through CAI the students having different languages were different on academic achievement and interest while no difference was seen on retention. In control group taught through lecture method the students having different languages were different in academic achievement and retention while no difference was seen on interest. Recommendations: This research found that CAI method of teaching is better than the traditional one; hence the government should allocate a lot of money to develop the teachers’ professionalism. This research found that CAI method of teaching is better than the traditional one in enhancing the academic achievement, interest and retention of the students, therefore proper planning, inspection and support should be provided by the government for the successful utilization of computers for instructional purpose. Suitable software should be made available at vii the local levels that cover the contents in different subjects. The present study was at the secondary level which found that CAI is a better method than traditional one; therefore other studies can be conducted at different other levels like primary, higher secondary and university level to see the effects of CAI. Studies can also be conducted in other science subjects such as chemistry and biology and in different other areas like mathematics and languages.