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Impact of Biochar on Chromium Uptake by Maize Grown in Tannery Polluted Soil

Thesis Info

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Author

Bashir, Muhammad Asaad

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Soil Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12890/1/PhD_Thesis_2006-ag-1361_%28Muhammad_Asaad_Bashir%29%5b1%5d.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726410445

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Leather industry is one of the leading industries in Pakistan. However, environmental management for this industry is generally ignored. Chromium (Cr) is a heavy metal used for tanning of leather and discharged into soil through waste water. It is toxic to plants and animals even at low concentration and causes human health hazards through food chain. Organic amendments such as compost and biochar are alternatives to expensive and could lower the pollutant associated risk. Elemental sulfur (ES) as inorganic amendments could alter the Cr dynamics and lowered the toxicity by adsorption on biochar surfaces. A survey of tannery area of District Kasur (K) and Sialkot (S) was conducted to check the Cr toxicity level in different location and soil was collected. The present research project was planned with four independent experiments focusing on major issues: (i) Evaluation of feedstock and pyrolytic temperature for biochar production on the basis of maximum adsorption of Cr (ii) Effect of biochar on different maize cultivars grown in tannery polluted soil (iii) Impact of biochar with compost on Cr tolerance in maize cultivars (iv) Effect of biochar with elemental sulfur on Cr dynamics in soil to reduce uptake in maize. In evaluation of biochar, sugarcane bagasse (SB) prepared at 350oC applied at rate of 3% maximum adsorb the Cr and improved the soil pH, electrical conductivity (EC), cation exchange capacity (CEC) and organic carbon (OC). After development of best adsorbent biochar, it was further evaluated on different maize cultivars grown in tannery polluted soil. Biochar application in both K and S soil were significantly improved the plant growth, physiological and biochemical attributes and decreased the concentration of Cr (VI) and Cr (III) in maize cultivars. Cultivar P-1543 performed better followed by NK-8441, NK-8711 and FH-985 in both K and S soils. Furthermore, biochar was evaluated with compost (1:1) on Cr tolerance in maize cultivars (P-1543 and NK-8441) grown in tannery polluted soil. It was observed that combined application of biochar and compost showed better results than their sole application. In comparison of both soil, Cr toxicity were more effected the maize growth in Sialkot soil. In the last trial, more polluted Sialkot soil was ameliorated. Biochar was compared with ES on Cr dynamics in tannery polluted K and S soil. It was observed that biochar application with ES improved the maize growth by reduced the toxicity of Cr in K as well S Soil. Conclusively, biochar (SB-350oC) application at rate of 3% was effective in amelioration of tannery polluted soil and combine application of biochar with compost and ES were better improved the maize growth.
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۱-حروف استفہام

۱-حروف استفہام

وہ حروف جو سوال کرنے کے لیے استعمال ہوتے ہیں یہ دو ہیں:ھل اور ھمزہ

قرآن حکیم میں متعدد مقامات پر وہ مستعمل ہیں مثلاً

" أَأَمِنتُمْ مَّن فِى ٱلسَّمَآءِ أَن يَخْسِفَ بِكُمُ ٱلأَرْضَ فَإِذَا هِىَ تَمُورُ " [[1]]

"کیا تم آسمان والے (رب) سے بے خوف ہو گئے ہو کہ وہ تم پر پتھر برسانے والی ہوا بھیج دے"۔

" هَلْ فِى ذٰلِكَ قَسَمٌ لِّذِى حِجْرٍ "[[2]]

"کیا اِس میں کسی صاحب عقل کے لیے کوئی قسم ہے؟"۔



[[1]]     القرآن ،۶۷: ۱۶۔

[[2]]     القرآن ، ۸۹: ۵۔

Comparative Analysis of AC and DC Distribution System with Respect to Harmonic Distortion Considering Daily Load Profile

Electrical energy is the most efficient and the cleanest form of energy at the moment that is being transmitted and distributed amongst end-users. From its earlier days, the AC system was preferred as an economical solution for transmission and distribution. However, the development in the power electronics technology and the evolution of highly efficient power electronic converters have established the resurgence of DC power system. Furthermore, the trend is shifting towards DC loads as various energy efficient appliances, such as DC inverter air conditioners, operate on DC nowadays. This further advocates the shift towards the DC power system. This research works is an effort to perform the comparative analysis of AC Distribution System (ACDS) and DC Distribution System (DCDS), with regards to power quality and harmonic distortion in particular. The comparison is performed considering load profile and load variation on daily basis. Simulations are performed in MATLAB. It has been concluded at the end that ACDS is better than DCDS in terms of power quality as total harmonic distortion of the DCDS under the same loading and same load variation during the whole day was significantly higher than that of ACDS.

Characteristics of Heavy Metal Uptake and Accumulation in Selected Plant Species for Development of Phytoextraction Technology.

soil, especially agricultural fields contaminated with toxic metals, has become a global problem and demands economic, efficient and environment friendly remediation technologies. Phytoextraction is a potential plant-based technology for the decontamination of polluted soil and water. It is an economic, solar driven, and environment friendly technology. In the present study, physiological, biochemical and molecular characteristics of cadmium uptake and accumulation in three plant species (Parthenium hysterophorus, Ricinus communis and Cannabis sativa) were studied for the development of phytoextraction technology. In the first experiment, different treatments of Gibberellic Acid (GA3 10−2, 10−4 and 10−6 M as foliar spray) and Ethylenediaminetetraacetic acid (EDTA 40 mgKg-1 soil as single dose and 10 mgKg-1 soil given in four doses) were studied for their effects on Cd phytoextraction, and concentration of proline, phenolics and chlorophyll in Parthenium hysterophorus plants grown in Cd (100 ppm) contaminated soil. The plants showed Cd hyperaccumulator potential based on Cd bio-concentration factor (BCF > 1 in control plants). The GA3 and EDTA application increased the extent of Cd phytoextraction by the plants. Most significantly increase in Cd accumulation and bio concentration (BCF 9.75 ± 0.34) were found in plants treated with GA3 (10−2 M) in combination with split doses of EDTA. Gibberellic acid significantly increased the concentrations of phenolics and chlorophyll in the plants. Cadmium accumulation in plant tissues showed positive correlation with free proline (R2 = 0.527, R2= 0.630) and total phenolics (R2 = 0.554, R2 = 0.723) in roots and leaves, respectively. In the second experiment, physiological and biochemical analyses were performed. The objectives were to assess the effect of Molybdenum (Mo 0.5, 1.00 and 2.00 ppm) on Cd phytoextraction, and concentration of endogenous proline, phenolics and photosynthetic in Ricinus communis and Cannabis sativa plants grown in Cd (25, 50 and 100 ppm) contaminated soil. Molybdenum was applied as a foliar spray, soil addition and seed soaking. Foliar spray of Molybdenum highly increased Cd uptake and accumulation in both plants. Molybdenum seed soaking and foliar spray highly increased the biomass, concentration of free proline and total phenolics as compared to control plants. Positive correlations of proline and phenolics with Cd accumulation were found in roots and leaves; suggesting a significant role of proline and phenolics in Cd phytoaccumulation. Molecular investigation was carried out with objectives: (1) To determine the presence and then expression of DREB-1A, DREB-1B, DREB-1F and CBF like genes in Ricinus communis and Cannabis sativa plants, (2) To evaluate the effect of molybdenum and cadmium on expression of these genes, (3) To correlate the expression of genes with Cd accumulation, and free proline and total phenolics concentrations in plants. Molybdenum was applied as a foliar spray (0.5, 1, 2 ppm) while Cd (50 ppm) was added to soil. cDNA was synthesized through reverse transcriptase Polymerase chain reaction (RT-PCR). PCR from genomic DNA and cDNA with genes specific primers were performed. The PCR products were sequenced and compared the nucleotide sequences and deduced amino acid sequences for homology with other plants. Results confirmed the presence of DREB-1A, DREB-1B, DREB-1F and CBF like genes in R. communis. In C. sativa the genes were identified for the first time. Cadmium induced the expression of DREB-1B, DREB-1F and CBF like genes in both the plants while molybdenum foliar spray further increased the expression of these genes under Cd stress. The DREB-1A showed no expression in both the plants while its presence was confirmed by the PCR product of genomic DNA with gene specific primers. The CBF-like gene was expressed in both the plants sprayed with molybdenum (without Cd), while the other genes were not expressed with Mo only. The DREB-1F and CBF like gene of both the plants showed more than 80 % nucleotide sequence homology with these genes in other plants. Predicted amino acid sequence of DREB-1F from both plant showed more than 75% homology with protein sequences of other plants, while the CBF like gene demonstrated more than 80% homology with protein sequences of other plants species. The expression of DREB-1B, DREB-1F and CBF-like genes (semi quantitative) was positively correlated with Cd accumulation, free proline and total phenolics in Cannabis sativa plant, while these correlations were significantly positive in Ricinus communis. Application of GA3 and Mo increased concentration of Cd in plants tissues. A positive inter-correlation was found between Cd concentration in plant tissues, production of prolin and phenolics and the expression of DREB-1B, DREB-1F and CBF-like genes.