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Home > Improvement in Drought Tolerance and Biomass of Transgenic Wheat Ectopically Expressing E. Coli Glutamate Dehydrogenase Gdha Gene

Improvement in Drought Tolerance and Biomass of Transgenic Wheat Ectopically Expressing E. Coli Glutamate Dehydrogenase Gdha Gene

Thesis Info

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Author

Butt, Aisha

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2015

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/6920/1/Aisha_Butt_Biotechnology_UAF_2015%20%281%29.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726430733

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Drought stress is the major limiting environmental factor in wheat yield. It is thought to be the most crucial factor that hinders crop productivity and poses problems to meet the increasing food demands. So, need is to develop drought tolerant and high yielding varieties to feed the ever increasing population. Wheat is highly calcitrant and poor responsive to tissue culture like other cereal crops. Recent study was conducted with the aims to establish an efficient regeneration protocol for development of transgenic wheat with improved drought tolerance and increased biomass. gdhA is a multifunctional gene reported to improve nitrogen and carbon metabolism, drought and herbicide tolerance and biomass. Regeneration protocol for wheat commercial cultivar Faisalabad-2008 was optimized using different combinations of 2,4-D, carbon source and age of calli. Better regeneratuion response was observed in 3 weeks old calli using 2 mg/L 2,4-D and maltose as carbon source. gdhA of E. coli was transformed using Agrobacterium mediated transformation method. Drought tolerance of putative transgenics calli was evaluated in vitro using different levels of PEG6000. Molecular analysis for presence and expression of transgene was performed. Transgenics alongwith control plants were subjected to different levels of drought stress. Transgenic plants showed 6.2, 13 and 10.1% more RWC and 5.6, 13.4 and 15.4% less saturation water deficit than control plants at moderate, medium, and high level of drought stress respectively. gdhA transgenic plants also showed 10.8% more excised leaf water retention and quick recovery from drought than non-transgenic controls. About 52.2 and 29.73% and 16.47 % more chlorophyll a, b and X+C contents were estimated in transgenic plants than controls. Effect of different levels of drought stress on plant pigments was also measured. Non-transgenic control plants showed 5.71, 20.39 and 15.89% more decrease in chlorophyll A and B contents at medium, mild and high level of drought stress. In control plants 44.74 and 67.39 % more increase in X+C contents in comparison to transgenics at low and medium level of drought stress while 70.98 % decrease was estimated at high level of drought stress. Regarding biomass related parameters were studied. Transgenic plants showed 10, 8.39, 9.69 and 8.02 % increase in number of fertile tillers, spikelets/spike, grains/spike and 1000 grain weight respectively than non-transgenic control plants. The ectopic expression of gdhA gene in transgenic plants resulted in improved drought tolerance and biomass.
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ڈاکٹر شہزاد احمدکی نقد نگاری

ڈاکٹر شہزاد احمد کی نقد نگاری
ڈاکٹر شہزاد احمد بنیادی طور پر ایک محقق اورتذکرہ نگار ہیں مذکورہ ہر دوجہات نے اُن کے تنقیدی سرمائے کو پس پشت ڈال دیا ہے ۔ دوسرا وہ خود بھی تدوین کی مصروفیات کے باعث عملی تنقید کی طرف توجہ نہ کرسکے۔ اُن کی تحریروں میں اُن کے تنقیدی تبصرے بکھرے ہوئے حالات میں ملتے ہیں۔ اگر وہ ذرا سی توجہ کرتے تو یقینا ایک جداگانہ مقام پر فائز ہوتے ۔
لاکھوں سلام میںتنقیدی نقوش
اُن کی تنقید کے ابتدائی نمونے اُن کے اولین تذکرے ’’لاکھوں سلام ‘‘ میں موجود ہیں۔ اس کتاب میں امام احمد رضا بریلوی کے سلام پر دس شعرا کی تضمینات جمع کی گئی ہیں اور شعراکا تعارف دیا گیا ہے۔ اس کے آغاز میں ہر شاعر کی نعتیہ شاعری اور شعری مجموعے پر ڈاکٹر شہزاداحمد نے اپنی تنقیدی آرا پیش کی ہیں جو لائق التفات ہیں،ان میں انھوں نے جن نکات کو اُجاگر کیا ہے۔ وہ درج ذیل ہیں:
۱۔ انتخاب الفاظ اور بندش کی کیفیت ۲۔ مضامین کا انداز اور تنوع
۳۔ جذبہ کی شدت کابیان ۴۔ کلام کی اثر آفرینی
۵۔ آداب نعت کی پاسداری ۶۔ موضوع اور اسلوب بیان
۷۔ مطالعہ سیرت کی ضرورت
مذکورہ خصوصیات میں سے زیادہ کا تعلق خالص شاعر ی سے ہے لیکن ساتھ ہی ایسے اصولوں کا ذکر ہے جو نعت کے ساتھ خاص ہیں۔ یہاں سے نعتیہ شاعری کے بارے میں ڈاکٹر صاحب کے جو اصول سامنے آئے ہیں، اُن میں پہلا یہ ہے کہ وہ نعت جیسے پاکیزہ موضوع کے لیے محتاط الفاظ اور سنجیدہ اسلوب اختیار کرنے کے قائل ہیں۔ وہ گورنر اُتر پردیش محمد عثمان عارف نقش بندی کے کلام پر رائے دیتے ہوئے لکھتے ہیں:
’’میں نے اپنے گرامی قدر استاد حضرت اخترالحامدی کے بارے میں عرض کیا ہے کہ نعت گوئی علم...

Tales Told by God: Stylistic Peculiarities of Qrua’nic Narratives.

Abstract The religious seminaries (Madaris) 1 of Pakistan have been a hot topic of discussion in the national and international media. These Madaris are reminiscent of the ancient education system of Islam. They attracted people’s attention during the last two decades and especially after the 11th September 2001 incident of New York. Another aspect of interest was their role in the Islamic insurgence (Jihad) waged in various parts of the world particularly in Afghanistan and Kashmir. These institutions impart orthodox education based on centuries old syllabus called Dars-i Nizami. The administrators are disinclined to any slight change in the contents of the syllabus thus bringing them to an open conflict with the modem education system and western culture. The Madaris have been divided on the basis of religious sects i. e. Shia, Sunni and Ahl-i-Hadith. Of them Sunnis are further splintered into many sub-sects such as Deobandis, Brelvis, etc. But their adherence to the primitive education, Jihad and abhorrence for the western civilization is almost the same. Despite repeated reference to these Madaris, there are many misconceptions or disinformation about them In the present paper, an attempt has been made to remove these misconceptions and present a true picture. Besides, historical perspective, vital statistics, curricula and problems and prospects have discussed in the paper in hand

Role of Halocarbons and Polychlorinated Biphenyls in the Development of Hepatitis

The present work is concerned with the determination of blood parameters, enzyme levels, blood chemistry and pesticide residues in the blood samples from different areas of Karachi in comparison with unexposed persons (i.e., control). Total 45 persons (exposed to pesticides) were taken in this study to assay the pesticides residues in their blood and also observed the morphological and chemical changes in the blood of affected persons. From 45 persons, only 20 persons were found positive and included in the study. Out of ten (10) standard pesticides five differentpesticides were identified in the blood of exposed persons such as cypermethrin, deltamethrin, polytrin-C, malathion and permethrin. The other standard pesticides such as DDE, DDT, match, diazinon and monocrotophos were not detected. In addition blood samples were also collected from ten (10) normal persons (not exposed to pesticides) for the determination of blood parameters, enzymes levels and blood chemistry. Regarding blood parameters approximately all the blood samples had significant variations i.e., (increase or decrease level) possibly due to the cumulative effect of pesticide residues e.g., Majid a young male aged 25 years had been exposed to pesticides for long time and therefore his TLC and platelet counts were found high in addition to low levels of RBC count, Hb, Hct and MCH which probably due to the excessive secretion of enzymes. Similarly, Ghulam Hussain another young male person aged only 08 years had low levels of RBC, Hb, Hct, platelet, MCV and MCH, which may be due to toxic effects of pesticides. In differential leukocyte count (DLC), granulocyte count was found in lower limits in 40 samples and showed normal limits in 05 samples. Lymphocyte count was xi found high in 31 samples. Low limit of lymphocyte was not found in any person. Similarly, monocyte count was found in high limits in 38 samples. Low monocyte count was not noted in any sample and the rest of the samples had normal limits of monocyte. Positive effects of pesticide residues were also observed on the enzyme levels. Regarding enzyme levels GPT, ALP, and ɣ - GT had higher levels in maximum number of samples. Cholinesterase was found high in 6 samples out of 45 samples and the highest was found in case of Farhan and Abdul Rahmanwhere as low limit of cholinesterase was noted in 15 samples out of total samples which may due to prolonged exposure of pesticides. GPT was found in high limits in all the samples. Also ɣ - GT and ALP found high in all the samples. Effect of pesticide residues were also observed on the blood chemistry. Regarding total bilirubin only one sample showed normal limit. The rest of the samples had raised total bilirubin and the highest was found in Javed a young male person aged 12 years. Similarly, in case of bilirubin direct all the blood samples showed increased level. The standard chromatograms of 10 different pesticides such as match, cypermethrin, deltamethrin, polytrin-C, diazinon, DDE, monocrotophos, DDT, malathion and permethrin were prepared on HPLC and their retention time (RT) was noted. Calculation was done for the quantity of pesticides residues from the peaks of chromatograms. Regarding pesticide residues, out of total 45 samples, only 20 persons were found positive for residues. The quantity of pesticide was determined by comparing xii the peak area of the standard pesticide with the sample peaks. After analysis, it was found that out of 20 samples deltamethrin and cypermethrin were found in noticeable amount in 6 samples. Also polytrin-C, malathion and permethrin were found in 2, 1 and 5 samples respectively. The pesticides such as match, diazinon, DDT and monocrotophoswere not detected in any sample.