The world health organisation (WHO) reported that Pakistan ranks fifth among highest tuberculosis (TB) burden countries. The present study was carried out on 366 cases, including 52% females and 48% males. The results showed that a higher percentage of patients with TB were between 16 to 30 years, having a body weight between 41 to 50 kg, in married, in uneducated people, having a high school education and in house wives. Out of 258 patients, 24% were diabetic, 17.8% were co-morbid with hepatitis C virus (HCV), 4.2% with human immunodeficiency virus (HIV) and 3.4% also had myocardial infarction. The TB patients revealed an increase in white blood cell counts (WBCs), erythrocyte sedimentation rate (ESR), alkaline phosphatase (ALP) and urea, while decrease in packed cell volume (PCV), eosinophils and immunoglobulin-G (IgG). The TB+ diabetes co-morbid group showed increases in WBCs, ESR, globulins, alanine transaminase (ALT), ALP, glucose, IgG and immunoglobulin-M (IgM), while a decrease in PCV, haemoglobin (Hb), eosinophil, albumin and albumin/globulin (A/G) ratio. The TB+ hepatitis co-morbid group showed increases in monocyte, ESR, ALT, ALP and IgG, while a decrease in PCV, Hb and eosinophil. The TB+HIV co-morbid group revealed an increase in ALP, IgG and IgM, while a decrease in monocyte and eosinophil. TB+ myocardial infarcted group showed increase in WBCs, neutrophil, ESR, serum proteins, globulin, ALP, serum cholesterol, high density lipoprotein (HDL), low density lipoproteins (LDL), creatinine kinase-MB (CKMB), creatinine phosphokinase (CPK), lactate dehydrogenase (LDH), creatinine and urea, while decrease in platelets (PLT) and lymphocyte. TB + diabetes + hepatitis C co-morbid cases showed increase in WBCs, ESR, bilirubin, ALT, AST, ALP, glucose, serum creatinine, serum urea, IgG, IgM, potassium and phosphorous, while a decrease in RBCs, PCV, Hb and eosinophil. TB + hepatitis C+AIDS co-morbid group showed increases in lymphocyte, monocyte, eosinophil, ALP, IgG and IgM, while a decrease in PCV, PLT and neutrophil. The prevalence of drug resistance by proportion method was 45.7%. Out of 118 drug resistant isolates, 61.02% were resistant to isoniazid (INH), 59.32% to ethambutol (EMB), 41.53% to streptomycin (SM), 5.08% to ofloxacin (OFX) and 49.15% were MDR detected by proportion method, while 31.36% isolates were resistant to INH, 22.03% to EMB, 17.08% to SM, 2.54% to OFX and 18.64% were MDR by PCR-RFLP. The consensus sequence alignment of three strains of KatG gene showed mutation at codon 282, 286, 279, 309 and 427. The change at codon 279 was observed in all the strains which added restriction site for MspI. The embB 306 showed mutation at codon 299, 300, while embB 497 at codon 70, 71, 76 and 78. The gyrA showed point mutation at codon 70, 71, 76, 78 and 95. It can be concluded from the present study that 24% TB patients were diabetic, 17.8% were co-morbid with hepatitis C, 4.2% with HIV and 3.4% also had myocardial infarction and 45.7% of cases were drug resistant.
یہ بھی ایک وقت تھا ہماری مائیں ہماری بہنیں عید سے پہلے ہماری رہائی کے لیے مظاہر ے کیا کرتی تھیں ہماری مائیں بھی کسی میکسم گورکی کی ماں سے کم نہیں تھیں ۔
آج حالات اس سے بدتر ہیں اب تو سیاسی قیدیوں کو غائب ہی کر دیا جا تا ہے مصطفی کانجو کو غائب کیے ہوئے پانچ مہینے ہو گئے ابھی تک کچھ پتہ نہیں کہ زندہ بھی ہے کہ نہیں۔ تصویر میں میری ماں شیخ عبدالقیوم کی بھتیجیاں اور دبنگ جیالی خالی سکینہ بادشاہ مرحومہ نمایاں ہیں جن کی بیٹی کی وڈیونیب ہیڈکواٹر کے باہر پولیس سے دست بدست لڑتے ہوئے وائرل ہوئی تھی ۔1986ء کمیٹی چوک راولپنڈی
The Quranic sciences of interpretations and principles of interpretations were originated in the epoch of holy prophet (saw) but compilation of both were started later. This is called principles of interpretations, which is the basic and important part of Islamic studies. Islamic scholars, commentators and explicators explained the holy Quran in the light of principles of interpretations. These are such basic principles those are guide lines for them so they will not deviate or drop the right way while interpreting and explaining the holy verses. These principles are laid down and followed by them so that they may find the will of Allah and actual meaning of holy Quran. A little difference in principles causes a huge difference in interpretation. Different methodology in principles resulted in many schools of thought. This article focusses on examining these schools of thought found in sub-continent and introduce their main books. This article deals with chronological evolution of said knowledge i.e. Principles of interpretations, and Quranic sciences specially originated by the famous principalities and interpreters of sub-continent.
Growth, external morphometry, proximate composition, and heavy metals concentration level in grass carp (Cetnopharyngodon idella) were studied. Different aspects of growth were studied in response to laboratory-prepared fish feeds having different crude levels of proteins provided to grass carp fingerlings. To study external morphometry and proximate composition, 72 samples of grass carp were randomly collected from the pond in which grass carp had shown best growth response to the feed which had 20% crude protein level . Fifty samples out of the total 72 samples were taken for evaluating concentration levels of twelve selected heavy metals. The study was conducted to assess the effect of three laboratory-prepared feeds having different levels of protein against control diet (containing rice polish only) on different aspects of growth of Ctenopharyngodon idella fingerlings. The trial period for this investigation was 90 days. Fingerlings were reared in four fish ponds, each having one acre surface area . Stocking density of the fingelings was 2000/acre. The fingerlings were hand fed @4% of body weight in the morning and evening during the trial period, six days a week on various diets T1, T2 ,T3 and T4(control) containing 15%, 20%, 25% crude protein level and rice polishing (control), respectively. Standardized methods were used in the whole trial. Average increase and percent increase in live weight and total length gain were monitored. Maximum daily growth rate, production, survival and specific growth rate were recorded in the fingerlings fed on a diet (T2) containing20% level of crude protein as compared to other diets having low or high crude protein levels. The lowest food conversion ratio (FCR) value of6.64 revealed T2 as the best feed composition. The diet having 20% crude protein showed maximum growth performance, specific growth rate, production, survival rate, etc.; therefore, T2 was the best feed composition for optimum growth of Ctenopharyngodon idella fish. Various morphometric characters, such as standard length (SL), head length (HL), head width (HW). body girth (BG), body depth (BD), dorsal fin length (DFL), forked length (FL), pectoral fin length (PtFL), pelvic fin length (PvFL), anal fin length (AFL), eye diameter (ED), tail length or caudal fin length (CFL), tail width (TW), wet body weight (W) with reference to total length (TL), condition factor (K) were studied to elucidate their correlations and types of growth. Correlation coefficient (r) value of 0.984 showed highly significant relationship between total length and wet body weight, while growth was negative allometric as 2.97 value of b was less than ideal slope value (b = 3). Regression coefficient value of condition factor (K) was 1.20 ± 0.10, and significant relationship was observed between K and total length and body weight. Highly significant correlation (P < 0.001) was also found between body weight and various morphometric characters except for condition factor (K). The interrelationships of total length to SL, HL, HW, BG, BD, HW, BG, BD, DFL, PtFL, PvFL, AFL, ED, TL, TW were highly significant (P < 0.001). Proximate composition implies percent composition of water, protein, lipids, and carbohydrates. Standardized methods were used to find the proximate composition of 72 collected samples from a pond. Mean percent values for water were 80.76 %; ash 3.40 %; fat 4.31 %, and protein 11.53% in wet weight. Percent water showed inverse relationship with protein (dry & wet body weight), ash and organic contents (wet body weight). Wet body weight showed negative correlation with percent water, Percent fat (dry & wet body weight) and positive correlation with percent protein and ash (wet & dry weight). Total length showed negative correlation with percent water and fat (dry and wet body weight), and positive correlation with percent protein (wet and dry weight), ash and organic contents (wet body weight). Grass carp is rich source of protein, i.e. 58.35% in dry body weight, additionally, the pioneer reference on to proximate composition. To assess the levels of Ca, Cd, Cr, Co, Cu, Fe, K, Mn, Ni, Na, Pb, Zn; and their relationships to body size and condition factor, 55 fish samples were randomly selected and their concentrations were determined by ICP-OES after wet digestion method. Their concentrations were found to be within the normal limits. Cd, Pb, Cu, K, Mn, Ni, Na, increased isometrically; Cr, Co, Zn, had negative allometry, while Fe, Ca showed positive allometry with body weight increase. Ni, Mn, Pb, Cd, showed isometric increase; Na, K, Zn, Fe, Ca had positive allometry; while Co, Cu, Cr had negative allometry with total length increase. Ca, Cu, Fe, K, Mn, Na, Zn had highly significant relationships with body weight and total length. Most of inter- elemental relationships were found to be positive. Multiple regressions showed that Fe, Cu, Zn, Mn, Pb, Na, K, Ca, and Ni had highly significant relationship with total length and body weight. Since concentration of all the studied metals were within the limits set by FAO and WHO, therefore, intake of the studied fish is safe.