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Home > Interactive Effects of Pgpr and Pgr on the Drought Tolerance of Crop Plants Grown in Sandy Soil and Elucidation of Plant Metabolites

Interactive Effects of Pgpr and Pgr on the Drought Tolerance of Crop Plants Grown in Sandy Soil and Elucidation of Plant Metabolites

Thesis Info

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Author

Khan, Naeem

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Plant Physiology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/11122/1/Naeem%20Khan_Plant%20Physiology_2018_QAU_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726490585

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Worldwide demand for agricultural crop continues to escalate in response to increasing population and damage of prime cropland for cultivation, following soil erosion and urbanization. Research interest is diverted to utilize soils of low or marginal productivity for crop production. Moisture stress has negative effects on crop productivity. The plant growth promoting rhizobacteria (PGPR) and plant growth regulators (PGR) are vital for normal growth and development under moisture stress. The present study was carried out to investigate the role of PGPR and PGR (Salicylic acid and Putrescine) on the physiology of chickpea and wheat grown in sandy soil. Metabolic profiling of leaves of chickpea exposed to moisture stress and treated with consortium of PGR and PGPR were also carried out in order to correlate the role of plant metabolites under various treatments. Liquid chromatographyhigh resolution mass spectrometry (LC-HRMS) analyses was carried out to classify metabolites associated with water stress tolerance in chickpea. To identify the important metabolites associated with drought condition three statistical models i.e. significant analysis of metabolites (SAM), partial least square discriminant analysis (PLS-DA) and random forest (RF) were used. The PGPR, isolated from the rhizosphere of chickpea and wheat were characterized on the basis of colony morphology and biochemical characters viz. Gram staining, P-solubilisation, antibacterial and antifungal activities and catalases and oxidases activities. The PGPR were also screened for the production of indole-3-acetic acid (IAA), hydrogen cyanide (HCN), ammonia (NH3) and exopolysaccharides (EPS). The PGPR P1, P2 and P3 were isolated from the rhizosphere of chickpea while, P4, P5 and P6 were isolated from the rhizosphere of wheat grown in sandy soil. The isolated PGPR strains named as P1, P2, P3, P4, P5 and P6 were identified by 16S-rRNA gene sequencing as Bacillus subtilis, Bacillus thuringiensis, Bacillus megaterium, Planomicrobium chinense, Bacillus cereus and Pseudomonas fluorescence, respectively. The seeds of two chickpea varieties (Punjab Noor2009 and 93127) and wheat varieties (Galaxy-13 and Pak-13) differing in sensitivity to drought were soaked for 2-3 h prior to sowing in 24 h old cultures of isolates. The salicylic acid (SA) and putrescine (Put) were sprayed (150 mg/L), on 20 days old seedlings of both chickpea and wheat. For metabolic profiling of chickpea, leaves from well-watered, waterdeficit and PGPR and PGR treated plants were collected at midday, 14 days (time point 1 at 15% soil moisture content) and 25 days (time point 2 at 6% soil moisture content) after the induction of water stress.
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مسز سنہا

مسز سنہا

            مسٹر سنہا، ایڈیٹر ہندوستان ریویو (الہ آباد) کی زوجہ محترمہ مسز سنہا، جنھوں نے حال ہی میں وفات پائی ہے، اپنی وفات سے چند روز پیشتر وصیت نامہ تحریر کرگئی تھیں، جس کی رو سے ایک ایک لاکھ روپیہ لاہور، الہ آبا د اور پٹنہ کی یونیورسٹیوں کو پہنچنا ہے، لاہور او ر الہ آباد کی یونیورسٹیاں اس سرمایہ سے ایک ایک پروفیسر شپ قائم کریں گی اور پٹنہ یونیورسٹی میں اس سے ایک کتب خانہ و دارالمطالعہ قائم ہوگا۔ (ستمبر ۱۹۱۹ء)

پاکستان میں غیر مسلموں کے جان و مال کا تحفظ، قرآن وسنت کی روشنی میں خصوصی مطالعہ

This fact is quite obvious from Quran o Sunnah that Islam is a religion of peace and provides guarantee of protection of life, property and self respect to the all members of society without any discrimination of colour, race and religion. It is the duty of an Islamic State to provide protection to the basic rights of all minorities. Holy Prophetﷺ said: "Beware! Whoever is cruel and harsh to a non-muslims minority, curtailing their rights, overburdening them or stealing from them, I will complain (to God) about that person on the day of judgement." Islam seeks to establish such a society where all citizens of the state enjoy equal rights and religion doesn't become the basis from any discrimination. Islamic law holds both muslims and non-muslims equal and no superiority or privilege is given to the muslims on any ground.

Integration of Humic Acid With Nitrogen for Improving Yield of Maize Genotypes

Soil, being an important constituent of crop production, serves as a major source of plant nutrients availability as well as metabolic processes which are indispensible for plant growth, development and survival. The integration of humic acid and inorganic fertilizers may help to fight the deficiency of essential nutrients and improve the soil fertility for better crop yield. Bearing in mind the above objectives, the current study was carried out at the Agricultural Research Station, Swabi for two consecutive years of 2014 and 2015. The experiment was carried out in randomized complete block (RCB) design using split-split plot arrangement and three replications. Four maize genotypes (3025, 55w65, Jalal and Iqbal), four N levels (0, 120, 180 and 240 kg N ha-1) and four treatments of humic acid (0, 0.6, 1.2 and 1.6 kg HA ha-1) were included in it. Regarding maize genotypes, late emergence, maximum days to 50% tasseling, 50% silking, anthesis to silking interval (ASI), days to physiological maturity, seed fill duration (SFD), leaves plant-1, leaf dry weight plant-1 at silking, leaf area plant-1 at silking, leaf area index (LAI) at silking, absolute growth rate (AGR) and crop growth rate (CGR) at silking, plant biomass, plant height, yield and yield components, harvest index (HI), agronomic-nitrogen use efficiency (NUE-AE), partial factor productivity-nitrogen use efficiency (NUE-PFP) and net income (NI) were recorded for hybrid 55w65, while higher AGR as well as CGR at maturity and productive plants m-2 were calculated for hybrid 3025. However, maximum specific leaf area (SLA) plant-1, leaf area ratio (LAR) plant-1, net assimilation rate (NAR) (30-75 DAS), soil attributes (organic matter, P and N) at maize harvest and grain protein were observed for Iqbal variety. As far as humic acid (HA) treatments are concerned, maximum days to physiological maturity, SFD, leaves plant-1, leaf dry weight plant-1 at silking, leaf area plant-1, LAI, AGR, CGR at silking, plant biomass, plant height, AGR, CGR at maturity, productive plants m-2, yield and yield components were recorded at 1.8 kg HA ha-1. Furthermore, humic acid at 1.8 kg ha-1 provided higher grain yield, biological yield, harvest index, NUE-AE, NUE-PFP, NAR (30-75 DAS), soil attributes at maize harvest, grain protein and net income, while it resulted in lower SLA and LAR plant-1 at silking. Similarly, 1.8 kg HA ha-1 had earlier 50% tasseling and 50% silking, whereas higher SLA and LAR plant-1 were recorded for control plots of humic xv acid (0 kg HA ha-1). While considering the impact of nitrogen levels, delayed physiological maturity, SFD, higher number of leaves plant-1, leaf dry weight plant-1, leaf area plant-1, LAI, AGR, CGR at silking, plant biomass, plant height, AGR, CGR at maturity, productive plants m-2, yield and yield components, harvest index, NAR (30-75 DAS), soil attributes at maize harvest, grain protein and net income were observed at 240 kg ha-1 N, while it resulted in lower SLA and LAR plant-1 at silking. Similarly, 240 kg ha-1 N application induced earliness in days to 50% tasseling, 50% silking and shortened the ASI. Likewise, higher SLA and LAR plant-1 at silking were calculated at 0 kg ha-1 N. Moreover, higher NUE-AE was achieved at 180 kg ha-1 N while NUE-PFP was higher under 120 kg N ha-1. The integration of humic acid with nitrogen increased the number of days taken by the maize genotypes to attain their physiological maturity, SFD, the number of leaves plant-1, leaf dry weight plant-1, leaf area plant-1, leaf area index, AGR, CGR at silking, plant biomass, plant height, AGR, CGR at maturity, productive plants m-2, yield and yield components, harvest index, NAR (30-75 DAS), soil attributes at maize harvest, grain protein and net income. Moreover, the number of days to emergence, 50% tasseling, 50% silking and ASI were also reduced when the inorganic fertilizer of N was supplemented by humic acid in maize genotypes, with subsequent improvement in their NUE-AE, NUE-PFP. However, the SLA and LAR of maize genotypes exhibited a downward trend with the application of humic acid + N. Thus it is finally concluded that 240 kg N ha-1 + 1.8 kg HA ha-1 along with hybrid maize 55w65 may be used for obtaining higher grain yield and improving soil organic matter status under the agro-climatic conditions of Swabi region on sustainable basis.