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Investigation on the Antioxidant and Antimutagenic Potential of Selected Indigenous Medicinal Plants.

Thesis Info

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Author

Yasir, Muhammad

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2015

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9840/1/Muhammad_Yasir_Chemistry_UAF_2015.docx

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726514279

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The present study has been designed to investigate the antioxidant and antimutagenic potential of hydrolyzed and non-hydrolyzed extracts of twenty medicinal plants belonging to different plant families like Amaranthaceae, Cucurbitaceae, Labiatae, Fabaceae, Solanaceae, Poaceae, Malvaceae and Zygophyllaceae. Hydrolyzed extraction was carried out using acidified methanol and non-hydrolyzed extracts were obtained with aqueous methanol. Both types of extracts were evaluated for phenolic contents, antioxidant, mutagenic and antimutagenic potential and genoprotective activity. Hydrolyzed extracts of all plants represented higher phenolic contents, antioxidant and antimutagenic potential as compared to non-hydrolyzed extracts. Mentha longifolia (402.8±1.9 mg GAE/g dry extract), Citrullus colocynthus (396.4±1.9 mg GAE/g dry extract) and Mimordica dioca (354.4±1.9 mg GAE/g dry extract) showed highest phenolic contents. Cassia angustifolia (230.4±2.6 mg AAE/g dry extract), Mimordica dioca (132.6±0.8 mg AAE/g dry extract) and Mentha longifolia (131.7±1.5 mg AAE/g dry extract) presented largest reducing power in terms of ascorbic acid equivalents (AAE). Highest free radical scavenging was shown by Citrullus colocynthus (96.21±1.0%; IC50 2.11±0.12 µg/mL), Achyranthus aspera (93.1±0.4%; IC50 6.5±0.10 µg/mL), Mentha longifolia (92.4±1.0%; IC50 14.3±0.1 µg/mL). Citrullus colocynthus (96.21±1.0%; IC50 2.11±0.12 µg/mL), Mimordica dioca (91.91±1.0%; IC50 17.22±0.36 µg/mL) and Mentha longifolia (92.4±1.0%; IC50 14.3±0.1 µg/mL) exhibited highest antioxidant activity in terms of inhibition to lipid peroxidation. Lowest cytotoxicity was observed for Citrullus colocynthus (2.65±0.09%), Achyranthus aspera (2.95±0.14%) and Mentha longifolia (3.95±0.08%) by evaluating haemolytic activity. Highest antimutagenic potential was calculated for hydrolyzed extracts of Citrullus colocynthus (75.79±0.69%), Mimordica dioca (73.02±1.37%) and Achyranthus aspera (72.22±0.69%). Also, Citrullus colocynthus, Mimordica dioca and Achyranthus aspera showed considerable protection against oxidatively damaged DNA plasmid i.e. pBR322. Finally, it has been concluded that Citrullus colocynthus, Mentha longifolia, Mimordica dioca and Achyranthus aspera were the most bioactive plants of all selected medicinal plants as represented by the results of LCMS analysis that exhibited the presence of high concentrations of bioactive compounds in the extracts of these plants.…………………………………
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المبحث الأول: فصول السنة والمشاعر

المبحث الأول: فصول السنة والمشاعر

قصیدة )کآبة الفصول الأربعة (لنازک الملائكة [1]

نحن نحیا في عالم کلّہ دم

عٌ وعمرٌ یفیض[2] یأساً وحزناً

تتشفی[3] عناصر الزمن القا

سي بأھاتنا وتسخر منّا

في غموض الحیاۃ نسرب[4] کالأ ش

باح بین البکاء والآھاتِ

کلّ یومٍ طفلٌ جدیدٌ ومیتٌ

ودموعٌ تبکي علی المأساۃ

ثم ماذا؟ في أيّ عالمنا المح

زن نلقٰی العزاء[5] عمّا نقاسي؟

عند وجہ الطبیعۃ الجھم [6] أم عن

د فؤاد الزمان وھو القاسي

قد عبرنا نھر الحیاۃِ حیارَی

في ظلام الفصول والسنوات

وثبتنا علیٰ أسانا خریفاً

وربیعاً فما جمالُ الحیاۃ؟

طالما مرّ بي الخریفُ فأصغي

تُ لصوتِ القمریّۃ[7] المحزونِ

وأنا في سکون غرفتي الدج

یاء أرنو[8] إلی وجوم[9] الغصون

طالما في الخریفِ سرت الی الحق

ل وأمعنتُ في وجومي[10] وحزني

کیف لا والکآبۃُ المرّۃ الخر

ساءُ قد رفرفتُ علی کلّ غصن

والحمامُ الجمیلُ قد ھَجَر الاّع

شاش سأمان [11]من وجوم السھوب

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