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Home > Isolation and Characterization of Cadmium Metallothionein Gene from Local Ciliates

Isolation and Characterization of Cadmium Metallothionein Gene from Local Ciliates

Thesis Info

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Author

Nighat, Rukhsana

Program

PhD

Institute

University of the Punjab

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2009

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1086

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726529813

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First cadmium metallothionein like gene PMCd1 of a ciliate, Paramecium sp., isolated from indus- trial wastewater has been cloned and sequenced. PMCd1 is an intronless gene, encoding 612 nucleotides, with TAA coding for glutamine. The coding region of PMCd1 com- prises 203 amino acids, including 37 cysteine residues with a conserved structural pattern in the form of recurring structural motifs, arranged in 17 x-cys-x-y-cys-x, 1 x-cys- cys-x and x-cys-x contexts. Both, the deduced amino acids and nucleotide sequence differ, not only from other animal metallothioneins (MTs), but also from the previously characterized Tetrahymena Cu and Cd-MTs. The translated protein of PMCd1 contains conserved cysteine residues, peculiar characteristic of stress inducible metallothionein genes of ciliates and other groups of organisms.
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