Magnetotactic bacteria (MTB) have ability to accumulate biominerals intracellularly as iron nanoparticles in the form of ferric oxide (Magnetite) and ferric sulphide (Greigite), known as magnetosomes. Most of them belong to the α-proteobacteria, β-proteobacteria and γ-proteobacteria class. In Pakistan, the Magnetotactic bacterial strains are not yet isolated. In current study, the Magnetotactic bacterial strains are isolated from different environmental samples and a modified specific growth medium was used to culture the isolated MTB strains. The technique used for accumulation of bacteria in water samples was the magnetic enrichment technique. The isolation of magnetotactic bacteria is difficult and identified number of strains of magnetotactic bacteria is less than a hundred. So, there is a need to focus more on the identification and classification of MTB. The selected strains were cultured on scale up production and extraction of magnetosomes were carried out by boiling method. The extracted Magnetosomes were characterized by Fourier Transform Infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray Powder diffraction (XRD).